Experiment: R2A with A22 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS03670 and CA265_RS03675 are separated by 25 nucleotides CA265_RS03675 and CA265_RS03680 overlap by 11 nucleotides CA265_RS03680 and CA265_RS03685 are separated by 2 nucleotides CA265_RS03685 and CA265_RS03690 are separated by 24 nucleotides
CA265_RS03670: CA265_RS03670 - O-acetyl-ADP-ribose deacetylase, at 875,495 to 876,016
_RS03670
CA265_RS03675: CA265_RS03675 - hypothetical protein, at 876,042 to 876,695
_RS03675
CA265_RS03680: CA265_RS03680 - RNA 2'-phosphotransferase, at 876,685 to 877,227
_RS03680
CA265_RS03685: CA265_RS03685 - serine/threonine protein phosphatase, at 877,230 to 877,961
_RS03685
CA265_RS03690: CA265_RS03690 - nicotinate phosphoribosyltransferase, at 877,986 to 879,443
_RS03690
Position (kb) 876
877
878 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 875.718 kb on - strand, within CA265_RS03670 at 875.864 kb on + strand, within CA265_RS03670 at 875.882 kb on - strand, within CA265_RS03670 at 875.882 kb on - strand, within CA265_RS03670 at 875.883 kb on + strand, within CA265_RS03670 at 875.884 kb on - strand, within CA265_RS03670 at 875.893 kb on + strand, within CA265_RS03670 at 875.984 kb on + strand at 876.018 kb on - strand at 876.018 kb on - strand at 876.108 kb on - strand, within CA265_RS03675 at 876.304 kb on + strand, within CA265_RS03675 at 876.304 kb on + strand, within CA265_RS03675 at 876.305 kb on - strand, within CA265_RS03675 at 876.333 kb on + strand, within CA265_RS03675 at 876.500 kb on + strand, within CA265_RS03675 at 876.538 kb on + strand, within CA265_RS03675 at 876.607 kb on + strand, within CA265_RS03675 at 876.694 kb on - strand at 876.711 kb on + strand at 876.711 kb on + strand at 876.723 kb on - strand at 876.755 kb on + strand, within CA265_RS03680 at 876.854 kb on - strand, within CA265_RS03680 at 876.854 kb on - strand, within CA265_RS03680 at 876.933 kb on + strand, within CA265_RS03680 at 876.933 kb on + strand, within CA265_RS03680 at 876.933 kb on + strand, within CA265_RS03680 at 876.934 kb on - strand, within CA265_RS03680 at 876.971 kb on + strand, within CA265_RS03680 at 876.972 kb on - strand, within CA265_RS03680 at 876.982 kb on + strand, within CA265_RS03680 at 876.982 kb on + strand, within CA265_RS03680 at 877.015 kb on - strand, within CA265_RS03680 at 877.086 kb on + strand, within CA265_RS03680 at 877.099 kb on - strand, within CA265_RS03680 at 877.116 kb on + strand, within CA265_RS03680 at 877.118 kb on + strand, within CA265_RS03680 at 877.216 kb on - strand at 877.218 kb on - strand at 877.243 kb on + strand at 877.247 kb on - strand at 877.250 kb on - strand at 877.256 kb on + strand at 877.257 kb on - strand at 877.257 kb on - strand at 877.306 kb on + strand, within CA265_RS03685 at 877.341 kb on + strand, within CA265_RS03685 at 877.373 kb on - strand, within CA265_RS03685 at 877.467 kb on - strand, within CA265_RS03685 at 877.471 kb on + strand, within CA265_RS03685 at 877.472 kb on - strand, within CA265_RS03685 at 877.472 kb on - strand, within CA265_RS03685 at 877.472 kb on - strand, within CA265_RS03685 at 877.508 kb on + strand, within CA265_RS03685 at 877.518 kb on + strand, within CA265_RS03685 at 877.656 kb on + strand, within CA265_RS03685 at 877.657 kb on - strand, within CA265_RS03685 at 877.669 kb on - strand, within CA265_RS03685 at 877.736 kb on - strand, within CA265_RS03685 at 877.801 kb on - strand, within CA265_RS03685 at 877.801 kb on - strand, within CA265_RS03685 at 877.803 kb on - strand, within CA265_RS03685 at 877.803 kb on - strand, within CA265_RS03685 at 877.803 kb on - strand, within CA265_RS03685 at 877.829 kb on + strand, within CA265_RS03685 at 877.830 kb on - strand, within CA265_RS03685 at 877.873 kb on + strand, within CA265_RS03685 at 877.922 kb on - strand at 877.974 kb on + strand at 877.980 kb on - strand at 877.999 kb on + strand at 877.999 kb on + strand at 878.020 kb on + strand at 878.021 kb on - strand at 878.048 kb on + strand at 878.101 kb on + strand at 878.150 kb on + strand, within CA265_RS03690 at 878.151 kb on - strand, within CA265_RS03690 at 878.162 kb on + strand, within CA265_RS03690
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.04 mg/ml remove 875,718 - CA265_RS03670 0.43 -0.3 875,864 + CA265_RS03670 0.71 -0.3 875,882 - CA265_RS03670 0.74 -0.5 875,882 - CA265_RS03670 0.74 -0.4 875,883 + CA265_RS03670 0.74 -0.1 875,884 - CA265_RS03670 0.75 -0.4 875,893 + CA265_RS03670 0.76 -0.6 875,984 + -0.1 876,018 - +0.6 876,018 - +0.3 876,108 - CA265_RS03675 0.10 -0.2 876,304 + CA265_RS03675 0.40 -0.2 876,304 + CA265_RS03675 0.40 +0.0 876,305 - CA265_RS03675 0.40 -0.8 876,333 + CA265_RS03675 0.44 +0.2 876,500 + CA265_RS03675 0.70 -0.5 876,538 + CA265_RS03675 0.76 +0.1 876,607 + CA265_RS03675 0.86 -0.3 876,694 - +1.0 876,711 + -1.6 876,711 + -0.2 876,723 - -1.4 876,755 + CA265_RS03680 0.13 -0.6 876,854 - CA265_RS03680 0.31 -0.2 876,854 - CA265_RS03680 0.31 +0.3 876,933 + CA265_RS03680 0.46 -0.7 876,933 + CA265_RS03680 0.46 +0.6 876,933 + CA265_RS03680 0.46 +0.1 876,934 - CA265_RS03680 0.46 -0.1 876,971 + CA265_RS03680 0.53 -0.6 876,972 - CA265_RS03680 0.53 -1.0 876,982 + CA265_RS03680 0.55 -1.1 876,982 + CA265_RS03680 0.55 +0.4 877,015 - CA265_RS03680 0.61 +0.1 877,086 + CA265_RS03680 0.74 -1.0 877,099 - CA265_RS03680 0.76 +0.4 877,116 + CA265_RS03680 0.79 -0.1 877,118 + CA265_RS03680 0.80 -0.6 877,216 - +0.5 877,218 - -0.5 877,243 + -0.1 877,247 - +0.1 877,250 - -2.7 877,256 + -0.9 877,257 - +0.2 877,257 - +0.3 877,306 + CA265_RS03685 0.10 -0.6 877,341 + CA265_RS03685 0.15 -0.9 877,373 - CA265_RS03685 0.20 -0.2 877,467 - CA265_RS03685 0.32 +1.4 877,471 + CA265_RS03685 0.33 -1.4 877,472 - CA265_RS03685 0.33 -0.6 877,472 - CA265_RS03685 0.33 -0.9 877,472 - CA265_RS03685 0.33 -0.5 877,508 + CA265_RS03685 0.38 -0.1 877,518 + CA265_RS03685 0.39 -0.1 877,656 + CA265_RS03685 0.58 -1.3 877,657 - CA265_RS03685 0.58 -0.7 877,669 - CA265_RS03685 0.60 -0.5 877,736 - CA265_RS03685 0.69 +1.3 877,801 - CA265_RS03685 0.78 -0.2 877,801 - CA265_RS03685 0.78 -2.2 877,803 - CA265_RS03685 0.78 -0.4 877,803 - CA265_RS03685 0.78 +1.3 877,803 - CA265_RS03685 0.78 +0.4 877,829 + CA265_RS03685 0.82 -0.2 877,830 - CA265_RS03685 0.82 -2.8 877,873 + CA265_RS03685 0.88 -0.0 877,922 - +0.4 877,974 + -0.3 877,980 - -0.7 877,999 + +1.1 877,999 + -0.4 878,020 + -0.3 878,021 - -1.1 878,048 + -0.2 878,101 + -0.1 878,150 + CA265_RS03690 0.11 -0.5 878,151 - CA265_RS03690 0.11 -0.1 878,162 + CA265_RS03690 0.12 -0.7
Or see this region's nucleotide sequence