Experiment: R2A with A22 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS03060 and CA265_RS03065 are separated by 238 nucleotides CA265_RS03065 and CA265_RS03070 are separated by 64 nucleotides CA265_RS03070 and CA265_RS03075 are separated by 9 nucleotides CA265_RS03075 and CA265_RS03080 are separated by 172 nucleotides
CA265_RS03060: CA265_RS03060 - DNA-binding response regulator, at 759,627 to 760,304
_RS03060
CA265_RS03065: CA265_RS03065 - hypothetical protein, at 760,543 to 761,079
_RS03065
CA265_RS03070: CA265_RS03070 - sec-independent protein translocase TatC, at 761,144 to 761,539
_RS03070
CA265_RS03075: CA265_RS03075 - hypothetical protein, at 761,549 to 761,974
_RS03075
CA265_RS03080: CA265_RS03080 - malate dehydrogenase, at 762,147 to 763,085
_RS03080
Position (kb)
761
762 Strain fitness (log2 ratio)
-1
0
1
2 at 760.203 kb on + strand, within CA265_RS03060 at 760.219 kb on - strand, within CA265_RS03060 at 760.220 kb on + strand, within CA265_RS03060 at 760.266 kb on + strand at 760.267 kb on - strand at 760.287 kb on + strand at 760.296 kb on + strand at 760.296 kb on + strand at 760.317 kb on - strand at 760.419 kb on - strand at 760.453 kb on - strand at 760.473 kb on - strand at 760.473 kb on - strand at 760.477 kb on + strand at 760.478 kb on - strand at 760.525 kb on + strand at 760.641 kb on + strand, within CA265_RS03065 at 760.765 kb on + strand, within CA265_RS03065 at 760.777 kb on - strand, within CA265_RS03065 at 760.783 kb on + strand, within CA265_RS03065 at 760.839 kb on + strand, within CA265_RS03065 at 760.840 kb on - strand, within CA265_RS03065 at 760.878 kb on + strand, within CA265_RS03065 at 760.993 kb on - strand, within CA265_RS03065 at 761.001 kb on + strand, within CA265_RS03065 at 761.020 kb on + strand, within CA265_RS03065 at 761.021 kb on - strand, within CA265_RS03065 at 761.099 kb on + strand at 761.236 kb on + strand, within CA265_RS03070 at 761.236 kb on + strand, within CA265_RS03070 at 761.324 kb on + strand, within CA265_RS03070 at 761.357 kb on - strand, within CA265_RS03070 at 761.359 kb on - strand, within CA265_RS03070 at 761.427 kb on - strand, within CA265_RS03070 at 761.447 kb on + strand, within CA265_RS03070 at 761.458 kb on + strand, within CA265_RS03070 at 761.468 kb on - strand, within CA265_RS03070 at 761.492 kb on - strand, within CA265_RS03070 at 761.492 kb on - strand, within CA265_RS03070 at 761.509 kb on + strand at 761.510 kb on - strand at 761.547 kb on + strand at 761.547 kb on + strand at 761.548 kb on - strand at 761.608 kb on + strand, within CA265_RS03075 at 761.609 kb on + strand, within CA265_RS03075 at 761.610 kb on - strand, within CA265_RS03075 at 761.610 kb on - strand, within CA265_RS03075 at 761.610 kb on - strand, within CA265_RS03075 at 761.626 kb on + strand, within CA265_RS03075 at 761.626 kb on + strand, within CA265_RS03075 at 761.631 kb on - strand, within CA265_RS03075 at 761.632 kb on + strand, within CA265_RS03075 at 761.632 kb on + strand, within CA265_RS03075 at 761.632 kb on + strand, within CA265_RS03075 at 761.633 kb on - strand, within CA265_RS03075 at 761.785 kb on + strand, within CA265_RS03075 at 761.805 kb on - strand, within CA265_RS03075 at 761.839 kb on + strand, within CA265_RS03075 at 761.851 kb on + strand, within CA265_RS03075 at 761.866 kb on + strand, within CA265_RS03075 at 761.866 kb on + strand, within CA265_RS03075 at 761.979 kb on + strand at 761.979 kb on + strand at 762.023 kb on - strand at 762.048 kb on + strand at 762.057 kb on - strand at 762.067 kb on + strand at 762.067 kb on + strand at 762.068 kb on - strand at 762.068 kb on - strand at 762.068 kb on - strand at 762.093 kb on - strand at 762.137 kb on + strand at 762.137 kb on + strand at 762.182 kb on + strand at 762.275 kb on + strand, within CA265_RS03080 at 762.286 kb on - strand, within CA265_RS03080 at 762.291 kb on + strand, within CA265_RS03080 at 762.300 kb on - strand, within CA265_RS03080 at 762.426 kb on + strand, within CA265_RS03080 at 762.458 kb on + strand, within CA265_RS03080 at 762.483 kb on - strand, within CA265_RS03080 at 762.501 kb on - strand, within CA265_RS03080 at 762.520 kb on + strand, within CA265_RS03080
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.04 mg/ml remove 760,203 + CA265_RS03060 0.85 -0.6 760,219 - CA265_RS03060 0.87 -0.4 760,220 + CA265_RS03060 0.87 +0.9 760,266 + +0.2 760,267 - +0.2 760,287 + +0.8 760,296 + -0.2 760,296 + +0.1 760,317 - +0.7 760,419 - -0.5 760,453 - -0.1 760,473 - -1.0 760,473 - +0.8 760,477 + +0.5 760,478 - -0.0 760,525 + +0.2 760,641 + CA265_RS03065 0.18 -0.5 760,765 + CA265_RS03065 0.41 +1.0 760,777 - CA265_RS03065 0.44 +2.7 760,783 + CA265_RS03065 0.45 -0.1 760,839 + CA265_RS03065 0.55 +0.3 760,840 - CA265_RS03065 0.55 -1.5 760,878 + CA265_RS03065 0.62 +0.8 760,993 - CA265_RS03065 0.84 -1.7 761,001 + CA265_RS03065 0.85 -1.4 761,020 + CA265_RS03065 0.89 +0.9 761,021 - CA265_RS03065 0.89 -0.5 761,099 + +0.1 761,236 + CA265_RS03070 0.23 -0.2 761,236 + CA265_RS03070 0.23 -0.8 761,324 + CA265_RS03070 0.45 +0.6 761,357 - CA265_RS03070 0.54 +0.2 761,359 - CA265_RS03070 0.54 +1.3 761,427 - CA265_RS03070 0.71 +0.2 761,447 + CA265_RS03070 0.77 -0.2 761,458 + CA265_RS03070 0.79 -0.6 761,468 - CA265_RS03070 0.82 -0.5 761,492 - CA265_RS03070 0.88 +0.4 761,492 - CA265_RS03070 0.88 -0.1 761,509 + +0.3 761,510 - +0.3 761,547 + +0.5 761,547 + +0.4 761,548 - -0.6 761,608 + CA265_RS03075 0.14 +0.3 761,609 + CA265_RS03075 0.14 +0.6 761,610 - CA265_RS03075 0.14 -1.1 761,610 - CA265_RS03075 0.14 -0.5 761,610 - CA265_RS03075 0.14 -0.5 761,626 + CA265_RS03075 0.18 -0.0 761,626 + CA265_RS03075 0.18 +1.0 761,631 - CA265_RS03075 0.19 +0.0 761,632 + CA265_RS03075 0.19 -0.7 761,632 + CA265_RS03075 0.19 +0.1 761,632 + CA265_RS03075 0.19 +0.6 761,633 - CA265_RS03075 0.20 -0.7 761,785 + CA265_RS03075 0.55 +0.1 761,805 - CA265_RS03075 0.60 +0.3 761,839 + CA265_RS03075 0.68 +1.0 761,851 + CA265_RS03075 0.71 +0.7 761,866 + CA265_RS03075 0.74 -0.1 761,866 + CA265_RS03075 0.74 +0.1 761,979 + +0.0 761,979 + -0.2 762,023 - -1.5 762,048 + +0.5 762,057 - +0.6 762,067 + -1.3 762,067 + +0.5 762,068 - +0.3 762,068 - +1.2 762,068 - -0.7 762,093 - -0.3 762,137 + +0.4 762,137 + +0.5 762,182 + -0.1 762,275 + CA265_RS03080 0.14 -0.7 762,286 - CA265_RS03080 0.15 +0.1 762,291 + CA265_RS03080 0.15 -0.5 762,300 - CA265_RS03080 0.16 -1.7 762,426 + CA265_RS03080 0.30 -1.1 762,458 + CA265_RS03080 0.33 -1.2 762,483 - CA265_RS03080 0.36 +0.0 762,501 - CA265_RS03080 0.38 -0.1 762,520 + CA265_RS03080 0.40 -0.5
Or see this region's nucleotide sequence