Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3343 and Shew_3344 are separated by 9 nucleotides Shew_3344 and Shew_3345 overlap by 1 nucleotides Shew_3345 and Shew_3346 are separated by 201 nucleotides Shew_3346 and Shew_3347 are separated by 2 nucleotides
Shew_3343: Shew_3343 - integral membrane sensor signal transduction histidine kinase (RefSeq), at 3,987,462 to 3,988,709
_3343
Shew_3344: Shew_3344 - dTDP-4-dehydrorhamnose reductase (RefSeq), at 3,988,719 to 3,989,642
_3344
Shew_3345: Shew_3345 - lipid kinase (RefSeq), at 3,989,642 to 3,990,565
_3345
Shew_3346: Shew_3346 - hypothetical protein (RefSeq), at 3,990,767 to 3,991,450
_3346
Shew_3347: Shew_3347 - hypothetical protein (RefSeq), at 3,991,453 to 3,993,333
_3347
Position (kb)
3989
3990
3991 Strain fitness (log2 ratio)
-1
0
1
2 at 3988.894 kb on + strand, within Shew_3344 at 3988.933 kb on + strand, within Shew_3344 at 3988.999 kb on + strand, within Shew_3344 at 3989.273 kb on - strand, within Shew_3344 at 3989.456 kb on - strand, within Shew_3344 at 3989.456 kb on - strand, within Shew_3344 at 3989.536 kb on - strand, within Shew_3344 at 3989.561 kb on - strand at 3990.096 kb on - strand, within Shew_3345 at 3990.274 kb on - strand, within Shew_3345 at 3990.295 kb on - strand, within Shew_3345 at 3990.686 kb on - strand at 3990.710 kb on - strand at 3990.782 kb on - strand at 3990.937 kb on - strand, within Shew_3346 at 3990.976 kb on - strand, within Shew_3346 at 3991.092 kb on + strand, within Shew_3346 at 3991.100 kb on - strand, within Shew_3346 at 3991.326 kb on - strand, within Shew_3346 at 3991.561 kb on - strand at 3991.563 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 3,988,894 + Shew_3344 0.19 +0.5 3,988,933 + Shew_3344 0.23 +0.2 3,988,999 + Shew_3344 0.30 -0.3 3,989,273 - Shew_3344 0.60 +0.2 3,989,456 - Shew_3344 0.80 -0.6 3,989,456 - Shew_3344 0.80 +0.3 3,989,536 - Shew_3344 0.88 -0.8 3,989,561 - +0.5 3,990,096 - Shew_3345 0.49 +1.0 3,990,274 - Shew_3345 0.68 +0.6 3,990,295 - Shew_3345 0.71 +0.1 3,990,686 - +0.4 3,990,710 - +0.5 3,990,782 - -0.2 3,990,937 - Shew_3346 0.25 +0.6 3,990,976 - Shew_3346 0.31 -0.2 3,991,092 + Shew_3346 0.48 -1.5 3,991,100 - Shew_3346 0.49 -0.0 3,991,326 - Shew_3346 0.82 +1.0 3,991,561 - +2.4 3,991,563 + +0.7
Or see this region's nucleotide sequence