Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2698 and Shew_2699 are separated by 221 nucleotides Shew_2699 and Shew_2700 are separated by 153 nucleotides Shew_2700 and Shew_2701 are separated by 82 nucleotides
Shew_2698: Shew_2698 - hypothetical protein (RefSeq), at 3,193,908 to 3,194,420
_2698
Shew_2699: Shew_2699 - cyclic nucleotide-binding protein (RefSeq), at 3,194,642 to 3,195,220
_2699
Shew_2700: Shew_2700 - antibiotic biosynthesis monooxygenase (RefSeq), at 3,195,374 to 3,195,763
_2700
Shew_2701: Shew_2701 - hypothetical protein (RefSeq), at 3,195,846 to 3,196,913
_2701
Position (kb)
3195
3196 Strain fitness (log2 ratio)
-1
0
1 at 3194.647 kb on - strand at 3194.663 kb on - strand at 3195.290 kb on - strand at 3195.338 kb on - strand at 3195.338 kb on - strand at 3195.338 kb on - strand at 3195.359 kb on + strand at 3195.407 kb on - strand at 3195.492 kb on - strand, within Shew_2700 at 3195.702 kb on + strand, within Shew_2700 at 3196.175 kb on - strand, within Shew_2701 at 3196.272 kb on + strand, within Shew_2701 at 3196.547 kb on - strand, within Shew_2701 at 3196.604 kb on - strand, within Shew_2701
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 3,194,647 - -1.5 3,194,663 - +0.8 3,195,290 - -0.2 3,195,338 - +0.0 3,195,338 - +0.1 3,195,338 - -0.2 3,195,359 + -0.0 3,195,407 - -0.1 3,195,492 - Shew_2700 0.30 +0.7 3,195,702 + Shew_2700 0.84 +0.6 3,196,175 - Shew_2701 0.31 +0.0 3,196,272 + Shew_2701 0.40 +0.1 3,196,547 - Shew_2701 0.66 -0.4 3,196,604 - Shew_2701 0.71 +0.1
Or see this region's nucleotide sequence