Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2660 and Shew_2661 are separated by 109 nucleotides Shew_2661 and Shew_2662 are separated by 136 nucleotides Shew_2662 and Shew_2663 are separated by 213 nucleotides
Shew_2660: Shew_2660 - hypothetical protein (RefSeq), at 3,134,052 to 3,135,284
_2660
Shew_2661: Shew_2661 - hypothetical protein (RefSeq), at 3,135,394 to 3,136,206
_2661
Shew_2662: Shew_2662 - cupin 2 domain-containing protein (RefSeq), at 3,136,343 to 3,136,702
_2662
Shew_2663: Shew_2663 - elongation factor G (RefSeq), at 3,136,916 to 3,138,964
_2663
Position (kb)
3135
3136
3137 Strain fitness (log2 ratio)
-1
0
1 at 3134.505 kb on - strand, within Shew_2660 at 3134.638 kb on - strand, within Shew_2660 at 3135.030 kb on - strand, within Shew_2660 at 3135.332 kb on + strand at 3135.399 kb on - strand at 3135.407 kb on + strand at 3135.669 kb on - strand, within Shew_2661 at 3135.954 kb on - strand, within Shew_2661 at 3136.161 kb on + strand at 3136.169 kb on - strand at 3136.383 kb on - strand, within Shew_2662 at 3136.383 kb on - strand, within Shew_2662 at 3136.432 kb on - strand, within Shew_2662 at 3136.540 kb on + strand, within Shew_2662 at 3136.576 kb on - strand, within Shew_2662 at 3137.073 kb on + strand at 3137.093 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 3,134,505 - Shew_2660 0.37 +0.2 3,134,638 - Shew_2660 0.48 -0.6 3,135,030 - Shew_2660 0.79 +0.4 3,135,332 + -1.3 3,135,399 - +1.3 3,135,407 + -0.2 3,135,669 - Shew_2661 0.34 -0.3 3,135,954 - Shew_2661 0.69 -1.5 3,136,161 + -1.1 3,136,169 - +0.4 3,136,383 - Shew_2662 0.11 +1.3 3,136,383 - Shew_2662 0.11 +0.1 3,136,432 - Shew_2662 0.25 -0.3 3,136,540 + Shew_2662 0.55 +0.1 3,136,576 - Shew_2662 0.65 -0.8 3,137,073 + -0.1 3,137,093 - -0.2
Or see this region's nucleotide sequence