Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_R0057 and Shew_R0058 are separated by 46 nucleotides Shew_R0058 and Shew_R0059 are separated by 47 nucleotides Shew_R0059 and Shew_R0060 are separated by 47 nucleotides Shew_R0060 and Shew_1812 are separated by 270 nucleotides
Shew_R0057: Shew_R0057 - tRNA-Met (RefSeq), at 2,082,317 to 2,082,393
_R0057
Shew_R0058: Shew_R0058 - tRNA-Met (RefSeq), at 2,082,440 to 2,082,516
_R0058
Shew_R0059: Shew_R0059 - tRNA-Met (RefSeq), at 2,082,564 to 2,082,640
_R0059
Shew_R0060: Shew_R0060 - tRNA-Met (RefSeq), at 2,082,688 to 2,082,764
_R0060
Shew_1812: Shew_1812 - 23S rRNA m(2)G2445 methyltransferase (RefSeq), at 2,083,035 to 2,085,170
_1812
Position (kb)
2082
2083 Strain fitness (log2 ratio)
-1
0
1 at 2081.770 kb on - strand at 2081.813 kb on + strand at 2081.813 kb on + strand at 2081.821 kb on + strand at 2081.821 kb on + strand at 2081.821 kb on - strand at 2081.821 kb on + strand at 2081.939 kb on - strand at 2083.137 kb on + strand at 2083.154 kb on + strand at 2083.169 kb on + strand at 2083.171 kb on + strand at 2083.247 kb on + strand at 2083.619 kb on - strand, within Shew_1812 at 2083.744 kb on + strand, within Shew_1812
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 2,081,770 - +0.6 2,081,813 + -0.4 2,081,813 + +0.0 2,081,821 + +0.2 2,081,821 + -0.4 2,081,821 - +0.4 2,081,821 + +0.1 2,081,939 - -0.5 2,083,137 + +0.4 2,083,154 + +0.4 2,083,169 + +0.3 2,083,171 + +0.7 2,083,247 + +0.1 2,083,619 - Shew_1812 0.27 +1.3 2,083,744 + Shew_1812 0.33 +1.5
Or see this region's nucleotide sequence