Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1729 and Shew_1730 are separated by 215 nucleotides Shew_1730 and Shew_1731 are separated by 262 nucleotides Shew_1731 and Shew_1732 are separated by 30 nucleotides
Shew_1729: Shew_1729 - curli production assembly/transport component CsgG (RefSeq), at 1,981,396 to 1,982,244
_1729
Shew_1730: Shew_1730 - hypothetical protein (RefSeq), at 1,982,460 to 1,983,026
_1730
Shew_1731: Shew_1731 - histidine triad (HIT) protein (RefSeq), at 1,983,289 to 1,983,639
_1731
Shew_1732: Shew_1732 - hypothetical protein (RefSeq), at 1,983,670 to 1,984,167
_1732
Position (kb) 1982
1983
1984 Strain fitness (log2 ratio) -1
0
1 at 1981.632 kb on + strand, within Shew_1729 at 1981.958 kb on + strand, within Shew_1729 at 1982.171 kb on + strand at 1982.956 kb on - strand, within Shew_1730 at 1983.389 kb on - strand, within Shew_1731 at 1983.660 kb on + strand at 1983.668 kb on - strand at 1983.806 kb on + strand, within Shew_1732 at 1983.991 kb on + strand, within Shew_1732
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 1,981,632 + Shew_1729 0.28 +0.4 1,981,958 + Shew_1729 0.66 +0.3 1,982,171 + -0.2 1,982,956 - Shew_1730 0.87 -1.5 1,983,389 - Shew_1731 0.28 +1.0 1,983,660 + +0.3 1,983,668 - +0.4 1,983,806 + Shew_1732 0.27 -0.3 1,983,991 + Shew_1732 0.64 +1.0
Or see this region's nucleotide sequence