Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1261 and Shew_1262 are separated by 140 nucleotides Shew_1262 and Shew_1263 are separated by 147 nucleotides Shew_1263 and Shew_1264 overlap by 4 nucleotides
Shew_1261: Shew_1261 - type III restriction enzyme, res subunit (RefSeq), at 1,457,762 to 1,460,347
_1261
Shew_1262: Shew_1262 - hypothetical protein (RefSeq), at 1,460,488 to 1,460,853
_1262
Shew_1263: Shew_1263 - hypothetical protein (RefSeq), at 1,461,001 to 1,461,984
_1263
Shew_1264: Shew_1264 - UvrD/REP helicase (RefSeq), at 1,461,981 to 1,464,017
_1264
Position (kb)
1461
1462 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1460.445 kb on - strand at 1461.019 kb on + strand at 1461.066 kb on - strand at 1461.076 kb on - strand at 1461.577 kb on + strand, within Shew_1263 at 1461.579 kb on + strand, within Shew_1263 at 1461.663 kb on - strand, within Shew_1263 at 1461.844 kb on - strand, within Shew_1263 at 1461.925 kb on + strand at 1462.061 kb on - strand at 1462.082 kb on + strand at 1462.191 kb on + strand, within Shew_1264
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 1,460,445 - +0.5 1,461,019 + +0.7 1,461,066 - -0.1 1,461,076 - +0.2 1,461,577 + Shew_1263 0.59 +1.1 1,461,579 + Shew_1263 0.59 +0.4 1,461,663 - Shew_1263 0.67 +0.5 1,461,844 - Shew_1263 0.86 +0.3 1,461,925 + -1.2 1,462,061 - -2.8 1,462,082 + -0.1 1,462,191 + Shew_1264 0.10 +0.2
Or see this region's nucleotide sequence