Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1196 and Shew_1197 are separated by 150 nucleotides Shew_1197 and Shew_1198 are separated by 97 nucleotides Shew_1198 and Shew_1199 are separated by 3 nucleotides Shew_1199 and Shew_1200 overlap by 8 nucleotides
Shew_1196: Shew_1196 - recombination and repair protein (RefSeq), at 1,387,289 to 1,388,950
_1196
Shew_1197: Shew_1197 - auxin efflux carrier (RefSeq), at 1,389,101 to 1,390,069
_1197
Shew_1198: Shew_1198 - hypothetical protein (RefSeq), at 1,390,167 to 1,390,361
_1198
Shew_1199: Shew_1199 - hypothetical protein (RefSeq), at 1,390,365 to 1,390,967
_1199
Shew_1200: Shew_1200 - hybrid sensory histidine kinase BarA (RefSeq), at 1,390,960 to 1,393,761
_1200
Position (kb)
1389
1390
1391 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1
2 at 1388.102 kb on - strand, within Shew_1196 at 1388.150 kb on + strand, within Shew_1196 at 1388.205 kb on - strand, within Shew_1196 at 1388.282 kb on - strand, within Shew_1196 at 1388.287 kb on - strand, within Shew_1196 at 1388.287 kb on - strand, within Shew_1196 at 1388.337 kb on + strand, within Shew_1196 at 1388.718 kb on + strand, within Shew_1196 at 1389.169 kb on - strand at 1389.298 kb on + strand, within Shew_1197 at 1389.306 kb on - strand, within Shew_1197 at 1389.306 kb on - strand, within Shew_1197 at 1389.306 kb on - strand, within Shew_1197 at 1389.306 kb on - strand, within Shew_1197 at 1389.355 kb on + strand, within Shew_1197 at 1389.355 kb on + strand, within Shew_1197 at 1389.355 kb on + strand, within Shew_1197 at 1389.420 kb on + strand, within Shew_1197 at 1389.425 kb on + strand, within Shew_1197 at 1389.514 kb on - strand, within Shew_1197 at 1389.623 kb on - strand, within Shew_1197 at 1389.828 kb on + strand, within Shew_1197 at 1390.127 kb on + strand at 1390.648 kb on + strand, within Shew_1199 at 1390.670 kb on - strand, within Shew_1199 at 1390.979 kb on + strand at 1391.031 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 1,388,102 - Shew_1196 0.49 -2.7 1,388,150 + Shew_1196 0.52 -4.5 1,388,205 - Shew_1196 0.55 -4.1 1,388,282 - Shew_1196 0.60 -4.0 1,388,287 - Shew_1196 0.60 -4.1 1,388,287 - Shew_1196 0.60 -2.1 1,388,337 + Shew_1196 0.63 -1.5 1,388,718 + Shew_1196 0.86 -7.0 1,389,169 - +0.5 1,389,298 + Shew_1197 0.20 +1.1 1,389,306 - Shew_1197 0.21 +0.3 1,389,306 - Shew_1197 0.21 +0.6 1,389,306 - Shew_1197 0.21 +1.8 1,389,306 - Shew_1197 0.21 -0.2 1,389,355 + Shew_1197 0.26 +0.3 1,389,355 + Shew_1197 0.26 -0.4 1,389,355 + Shew_1197 0.26 +0.1 1,389,420 + Shew_1197 0.33 +0.9 1,389,425 + Shew_1197 0.33 +0.5 1,389,514 - Shew_1197 0.43 +0.9 1,389,623 - Shew_1197 0.54 +1.6 1,389,828 + Shew_1197 0.75 +0.3 1,390,127 + +2.0 1,390,648 + Shew_1199 0.47 -0.3 1,390,670 - Shew_1199 0.51 -1.1 1,390,979 + -2.0 1,391,031 + -1.5
Or see this region's nucleotide sequence