Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1159 and Shew_1160 are separated by 184 nucleotides Shew_1160 and Shew_1161 are separated by 158 nucleotides Shew_1161 and Shew_1162 are separated by 312 nucleotides
Shew_1159: Shew_1159 - thioesterase superfamily protein (RefSeq), at 1,333,367 to 1,333,735
_1159
Shew_1160: Shew_1160 - hypothetical protein (RefSeq), at 1,333,920 to 1,334,126
_1160
Shew_1161: Shew_1161 - NADH dehydrogenase (RefSeq), at 1,334,285 to 1,335,586
_1161
Shew_1162: Shew_1162 - hypothetical protein (RefSeq), at 1,335,899 to 1,336,273
_1162
Position (kb)
1334
1335
1336 Strain fitness (log2 ratio)
-1
0
1 at 1333.582 kb on + strand, within Shew_1159 at 1333.585 kb on - strand, within Shew_1159 at 1333.622 kb on - strand, within Shew_1159 at 1333.727 kb on - strand at 1333.793 kb on - strand at 1333.796 kb on + strand at 1333.806 kb on + strand at 1333.814 kb on - strand at 1334.283 kb on + strand at 1334.653 kb on - strand, within Shew_1161 at 1334.826 kb on + strand, within Shew_1161 at 1335.445 kb on - strand, within Shew_1161 at 1335.952 kb on - strand, within Shew_1162 at 1335.970 kb on - strand, within Shew_1162 at 1336.391 kb on - strand at 1336.419 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 1,333,582 + Shew_1159 0.58 +0.3 1,333,585 - Shew_1159 0.59 +0.2 1,333,622 - Shew_1159 0.69 +0.3 1,333,727 - +0.2 1,333,793 - +1.1 1,333,796 + +0.7 1,333,806 + +0.1 1,333,814 - +0.0 1,334,283 + -0.1 1,334,653 - Shew_1161 0.28 +0.5 1,334,826 + Shew_1161 0.42 +0.1 1,335,445 - Shew_1161 0.89 +0.9 1,335,952 - Shew_1162 0.14 +0.3 1,335,970 - Shew_1162 0.19 -0.2 1,336,391 - +0.0 1,336,419 + -0.1
Or see this region's nucleotide sequence