Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0587 and Shew_0588 are separated by 83 nucleotides Shew_0588 and Shew_0589 are separated by 2 nucleotides
Shew_0587: Shew_0587 - phosphoribulokinase (RefSeq), at 664,811 to 665,698
_0587
Shew_0588: Shew_0588 - hypothetical protein (RefSeq), at 665,782 to 666,045
_0588
Shew_0589: Shew_0589 - alpha/beta hydrolase fold (RefSeq), at 666,048 to 667,031
_0589
Position (kb) 665
666
667 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 664.877 kb on + strand at 664.915 kb on + strand, within Shew_0587 at 665.028 kb on - strand, within Shew_0587 at 665.048 kb on + strand, within Shew_0587 at 665.053 kb on + strand, within Shew_0587 at 665.053 kb on + strand, within Shew_0587 at 665.053 kb on + strand, within Shew_0587 at 665.053 kb on + strand, within Shew_0587 at 665.061 kb on - strand, within Shew_0587 at 665.061 kb on - strand, within Shew_0587 at 665.068 kb on + strand, within Shew_0587 at 665.142 kb on - strand, within Shew_0587 at 665.142 kb on - strand, within Shew_0587 at 665.266 kb on + strand, within Shew_0587 at 665.308 kb on - strand, within Shew_0587 at 665.407 kb on + strand, within Shew_0587 at 665.423 kb on + strand, within Shew_0587 at 665.431 kb on - strand, within Shew_0587 at 665.780 kb on + strand at 665.780 kb on + strand at 665.920 kb on + strand, within Shew_0588 at 666.152 kb on + strand, within Shew_0589 at 666.160 kb on - strand, within Shew_0589 at 666.917 kb on + strand, within Shew_0589 at 666.946 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 664,877 + +0.5 664,915 + Shew_0587 0.12 +0.7 665,028 - Shew_0587 0.24 +0.2 665,048 + Shew_0587 0.27 -0.2 665,053 + Shew_0587 0.27 -0.2 665,053 + Shew_0587 0.27 +0.5 665,053 + Shew_0587 0.27 -0.2 665,053 + Shew_0587 0.27 +0.2 665,061 - Shew_0587 0.28 +1.5 665,061 - Shew_0587 0.28 +0.1 665,068 + Shew_0587 0.29 +0.1 665,142 - Shew_0587 0.37 +0.4 665,142 - Shew_0587 0.37 -0.2 665,266 + Shew_0587 0.51 +0.4 665,308 - Shew_0587 0.56 +1.1 665,407 + Shew_0587 0.67 -1.2 665,423 + Shew_0587 0.69 -0.3 665,431 - Shew_0587 0.70 +0.6 665,780 + -0.3 665,780 + +0.3 665,920 + Shew_0588 0.52 -1.5 666,152 + Shew_0589 0.11 -2.8 666,160 - Shew_0589 0.11 +1.0 666,917 + Shew_0589 0.88 -2.3 666,946 - -1.3
Or see this region's nucleotide sequence