Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tolC and Shew_0545 are separated by 263 nucleotides Shew_0545 and Shew_0546 overlap by 4 nucleotides Shew_0546 and Shew_0547 are separated by 9 nucleotides
Shew_0544: tolC - outer membrane channel protein (RefSeq), at 614,493 to 615,839
tolC
Shew_0545: Shew_0545 - ADP-ribose diphosphatase (RefSeq), at 616,103 to 616,711
_0545
Shew_0546: Shew_0546 - hypothetical protein (RefSeq), at 616,708 to 617,154
_0546
Shew_0547: Shew_0547 - calcineurin phosphoesterase C-terminal domain-containing protein (RefSeq), at 617,164 to 618,003
_0547
Position (kb) 616
617 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 615.922 kb on + strand at 615.992 kb on + strand at 616.296 kb on + strand, within Shew_0545 at 616.304 kb on - strand, within Shew_0545 at 616.347 kb on + strand, within Shew_0545 at 616.414 kb on - strand, within Shew_0545 at 616.439 kb on - strand, within Shew_0545 at 616.700 kb on - strand at 616.781 kb on - strand, within Shew_0546 at 616.795 kb on + strand, within Shew_0546 at 617.142 kb on + strand at 617.689 kb on + strand, within Shew_0547
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 615,922 + -4.1 615,992 + -0.7 616,296 + Shew_0545 0.32 -0.2 616,304 - Shew_0545 0.33 +1.0 616,347 + Shew_0545 0.40 +0.3 616,414 - Shew_0545 0.51 +1.0 616,439 - Shew_0545 0.55 +0.8 616,700 - +0.2 616,781 - Shew_0546 0.16 +0.6 616,795 + Shew_0546 0.19 +1.2 617,142 + +2.4 617,689 + Shew_0547 0.62 +1.1
Or see this region's nucleotide sequence