Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0101 and Shew_0102 are separated by 101 nucleotides Shew_0102 and Shew_0103 are separated by 206 nucleotides Shew_0103 and Shew_0104 are separated by 52 nucleotides Shew_0104 and Shew_0105 are separated by 75 nucleotides
Shew_0101: Shew_0101 - endoribonuclease L-PSP (RefSeq), at 111,320 to 111,721
_0101
Shew_0102: Shew_0102 - hypothetical protein (RefSeq), at 111,823 to 111,978
_0102
Shew_0103: Shew_0103 - sodium/glutamate symporter (RefSeq), at 112,185 to 113,408
_0103
Shew_0104: Shew_0104 - hypothetical protein (RefSeq), at 113,461 to 113,712
_0104
Shew_0105: Shew_0105 - peptidase M14, carboxypeptidase A (RefSeq), at 113,788 to 116,325
_0105
Position (kb) 112
113
114 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 111.225 kb on + strand at 111.413 kb on - strand, within Shew_0101 at 111.429 kb on + strand, within Shew_0101 at 111.450 kb on - strand, within Shew_0101 at 111.493 kb on + strand, within Shew_0101 at 111.745 kb on + strand at 111.879 kb on + strand, within Shew_0102 at 112.067 kb on - strand at 112.101 kb on - strand at 113.001 kb on - strand, within Shew_0103 at 113.193 kb on - strand, within Shew_0103 at 113.321 kb on - strand at 113.688 kb on + strand at 113.698 kb on - strand at 113.821 kb on + strand at 113.936 kb on - strand at 114.073 kb on - strand, within Shew_0105 at 114.378 kb on + strand, within Shew_0105
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 111,225 + +0.2 111,413 - Shew_0101 0.23 +0.1 111,429 + Shew_0101 0.27 +1.0 111,450 - Shew_0101 0.32 -0.3 111,493 + Shew_0101 0.43 +0.4 111,745 + -0.5 111,879 + Shew_0102 0.36 +0.4 112,067 - -1.8 112,101 - -0.5 113,001 - Shew_0103 0.67 -1.2 113,193 - Shew_0103 0.82 -3.2 113,321 - -0.5 113,688 + -0.4 113,698 - +0.1 113,821 + +0.3 113,936 - -0.1 114,073 - Shew_0105 0.11 +0.3 114,378 + Shew_0105 0.23 -0.1
Or see this region's nucleotide sequence