Experiment: octanoate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5221 and Pf6N2E2_5222 are separated by 14 nucleotides Pf6N2E2_5222 and Pf6N2E2_5223 overlap by 7 nucleotides Pf6N2E2_5223 and Pf6N2E2_5224 overlap by 8 nucleotides Pf6N2E2_5224 and Pf6N2E2_5225 are separated by 114 nucleotides
Pf6N2E2_5221 - Peptide chain release factor 1, at 6,019,919 to 6,021,001
_5221
Pf6N2E2_5222 - Protein-N(5)-glutamine methyltransferase PrmC, methylates polypeptide chain release factors RF1 and RF2, at 6,021,016 to 6,021,846
_5222
Pf6N2E2_5223 - Molybdopterin biosynthesis protein MoeB, at 6,021,840 to 6,022,595
_5223
Pf6N2E2_5224 - Glutamate racemase (EC 5.1.1.3), at 6,022,588 to 6,023,379
_5224
Pf6N2E2_5225 - Lipid A 3-O-deacylase, at 6,023,494 to 6,024,012
_5225
Position (kb)
6021
6022
6023 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 6020.999 kb on + strand at 6021.013 kb on + strand at 6021.013 kb on + strand at 6021.013 kb on + strand at 6021.978 kb on + strand, within Pf6N2E2_5223 at 6021.978 kb on + strand, within Pf6N2E2_5223 at 6021.978 kb on + strand, within Pf6N2E2_5223 at 6021.978 kb on + strand, within Pf6N2E2_5223 at 6022.271 kb on + strand, within Pf6N2E2_5223 at 6022.353 kb on + strand, within Pf6N2E2_5223 at 6022.365 kb on + strand, within Pf6N2E2_5223 at 6022.365 kb on + strand, within Pf6N2E2_5223 at 6023.334 kb on + strand at 6023.334 kb on + strand at 6023.334 kb on + strand at 6023.339 kb on + strand at 6023.436 kb on - strand at 6023.437 kb on + strand at 6023.466 kb on + strand at 6023.466 kb on + strand at 6023.466 kb on + strand at 6023.466 kb on + strand at 6023.466 kb on + strand at 6023.467 kb on - strand at 6023.467 kb on - strand at 6023.548 kb on + strand, within Pf6N2E2_5225 at 6023.548 kb on + strand, within Pf6N2E2_5225 at 6023.548 kb on + strand, within Pf6N2E2_5225 at 6023.548 kb on + strand, within Pf6N2E2_5225 at 6023.548 kb on + strand, within Pf6N2E2_5225 at 6023.548 kb on + strand, within Pf6N2E2_5225 at 6023.548 kb on + strand, within Pf6N2E2_5225 at 6023.548 kb on + strand, within Pf6N2E2_5225 at 6023.548 kb on + strand, within Pf6N2E2_5225 at 6023.549 kb on - strand, within Pf6N2E2_5225 at 6023.549 kb on - strand, within Pf6N2E2_5225 at 6023.549 kb on - strand, within Pf6N2E2_5225
Per-strain Table
Position Strand Gene LocusTag Fraction octanoate (C) remove 6,020,999 + +0.5 6,021,013 + +0.7 6,021,013 + +1.6 6,021,013 + -0.7 6,021,978 + Pf6N2E2_5223 0.18 +0.0 6,021,978 + Pf6N2E2_5223 0.18 -0.4 6,021,978 + Pf6N2E2_5223 0.18 -2.1 6,021,978 + Pf6N2E2_5223 0.18 -0.7 6,022,271 + Pf6N2E2_5223 0.57 -3.5 6,022,353 + Pf6N2E2_5223 0.68 -3.4 6,022,365 + Pf6N2E2_5223 0.69 -2.3 6,022,365 + Pf6N2E2_5223 0.69 -4.3 6,023,334 + -3.4 6,023,334 + -2.2 6,023,334 + -3.3 6,023,339 + +0.6 6,023,436 - +0.6 6,023,437 + +0.5 6,023,466 + +1.9 6,023,466 + +0.3 6,023,466 + +0.3 6,023,466 + +0.9 6,023,466 + -0.9 6,023,467 - +1.5 6,023,467 - -0.2 6,023,548 + Pf6N2E2_5225 0.10 +0.3 6,023,548 + Pf6N2E2_5225 0.10 -1.1 6,023,548 + Pf6N2E2_5225 0.10 +1.2 6,023,548 + Pf6N2E2_5225 0.10 -1.4 6,023,548 + Pf6N2E2_5225 0.10 -0.0 6,023,548 + Pf6N2E2_5225 0.10 -1.1 6,023,548 + Pf6N2E2_5225 0.10 +0.8 6,023,548 + Pf6N2E2_5225 0.10 +1.0 6,023,548 + Pf6N2E2_5225 0.10 +1.3 6,023,549 - Pf6N2E2_5225 0.11 -1.0 6,023,549 - Pf6N2E2_5225 0.11 +0.7 6,023,549 - Pf6N2E2_5225 0.11 +0.1
Or see this region's nucleotide sequence