Experiment: octanoate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3754 and Pf6N2E2_3755 are separated by 178 nucleotides Pf6N2E2_3755 and Pf6N2E2_3756 are separated by 31 nucleotides
Pf6N2E2_3754 - 3-dehydroquinate synthase (EC 4.2.3.4), at 4,374,837 to 4,375,937
_3754
Pf6N2E2_3755 - Shikimate kinase I (EC 2.7.1.71), at 4,376,116 to 4,376,607
_3755
Pf6N2E2_3756 - Type IV pilus biogenesis protein PilQ, at 4,376,639 to 4,378,729
_3756
Position (kb)
4376
4377 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4376.748 kb on + strand at 4376.776 kb on + strand at 4376.776 kb on + strand at 4376.776 kb on + strand at 4376.776 kb on + strand at 4376.776 kb on + strand at 4376.776 kb on + strand at 4376.777 kb on - strand at 4376.777 kb on - strand at 4376.777 kb on - strand at 4376.777 kb on - strand at 4376.780 kb on + strand at 4376.780 kb on + strand at 4376.780 kb on + strand at 4376.780 kb on + strand at 4376.780 kb on + strand at 4376.781 kb on - strand at 4376.781 kb on - strand at 4376.797 kb on + strand at 4377.161 kb on + strand, within Pf6N2E2_3756 at 4377.161 kb on + strand, within Pf6N2E2_3756 at 4377.161 kb on + strand, within Pf6N2E2_3756 at 4377.161 kb on + strand, within Pf6N2E2_3756 at 4377.162 kb on - strand, within Pf6N2E2_3756 at 4377.162 kb on - strand, within Pf6N2E2_3756 at 4377.162 kb on - strand, within Pf6N2E2_3756 at 4377.162 kb on - strand, within Pf6N2E2_3756 at 4377.199 kb on + strand, within Pf6N2E2_3756 at 4377.199 kb on + strand, within Pf6N2E2_3756 at 4377.355 kb on - strand, within Pf6N2E2_3756 at 4377.355 kb on - strand, within Pf6N2E2_3756 at 4377.355 kb on - strand, within Pf6N2E2_3756 at 4377.355 kb on - strand, within Pf6N2E2_3756 at 4377.355 kb on - strand, within Pf6N2E2_3756 at 4377.462 kb on + strand, within Pf6N2E2_3756 at 4377.462 kb on + strand, within Pf6N2E2_3756 at 4377.463 kb on - strand, within Pf6N2E2_3756 at 4377.576 kb on + strand, within Pf6N2E2_3756 at 4377.576 kb on + strand, within Pf6N2E2_3756 at 4377.576 kb on + strand, within Pf6N2E2_3756 at 4377.576 kb on + strand, within Pf6N2E2_3756 at 4377.576 kb on + strand, within Pf6N2E2_3756 at 4377.576 kb on + strand, within Pf6N2E2_3756 at 4377.577 kb on - strand, within Pf6N2E2_3756 at 4377.577 kb on - strand, within Pf6N2E2_3756 at 4377.577 kb on - strand, within Pf6N2E2_3756 at 4377.577 kb on - strand, within Pf6N2E2_3756 at 4377.577 kb on - strand, within Pf6N2E2_3756 at 4377.577 kb on - strand, within Pf6N2E2_3756 at 4377.577 kb on - strand, within Pf6N2E2_3756
Per-strain Table
Position Strand Gene LocusTag Fraction octanoate (C) remove 4,376,748 + -2.1 4,376,776 + -2.2 4,376,776 + -2.8 4,376,776 + -2.6 4,376,776 + -3.9 4,376,776 + +0.7 4,376,776 + -2.7 4,376,777 - -0.3 4,376,777 - +1.3 4,376,777 - +0.3 4,376,777 - -1.0 4,376,780 + -2.5 4,376,780 + -3.8 4,376,780 + -2.0 4,376,780 + -1.5 4,376,780 + -2.6 4,376,781 - +0.4 4,376,781 - +0.3 4,376,797 + -0.9 4,377,161 + Pf6N2E2_3756 0.25 +0.2 4,377,161 + Pf6N2E2_3756 0.25 +0.4 4,377,161 + Pf6N2E2_3756 0.25 -0.0 4,377,161 + Pf6N2E2_3756 0.25 +0.3 4,377,162 - Pf6N2E2_3756 0.25 +1.0 4,377,162 - Pf6N2E2_3756 0.25 +0.0 4,377,162 - Pf6N2E2_3756 0.25 +0.9 4,377,162 - Pf6N2E2_3756 0.25 +0.8 4,377,199 + Pf6N2E2_3756 0.27 +0.4 4,377,199 + Pf6N2E2_3756 0.27 +0.4 4,377,355 - Pf6N2E2_3756 0.34 -1.3 4,377,355 - Pf6N2E2_3756 0.34 +0.1 4,377,355 - Pf6N2E2_3756 0.34 +0.1 4,377,355 - Pf6N2E2_3756 0.34 +0.2 4,377,355 - Pf6N2E2_3756 0.34 +1.2 4,377,462 + Pf6N2E2_3756 0.39 -0.5 4,377,462 + Pf6N2E2_3756 0.39 +0.5 4,377,463 - Pf6N2E2_3756 0.39 +0.4 4,377,576 + Pf6N2E2_3756 0.45 -0.2 4,377,576 + Pf6N2E2_3756 0.45 +1.2 4,377,576 + Pf6N2E2_3756 0.45 +0.7 4,377,576 + Pf6N2E2_3756 0.45 -0.1 4,377,576 + Pf6N2E2_3756 0.45 -0.9 4,377,576 + Pf6N2E2_3756 0.45 -0.2 4,377,577 - Pf6N2E2_3756 0.45 +0.1 4,377,577 - Pf6N2E2_3756 0.45 +0.2 4,377,577 - Pf6N2E2_3756 0.45 +1.5 4,377,577 - Pf6N2E2_3756 0.45 -1.2 4,377,577 - Pf6N2E2_3756 0.45 +0.7 4,377,577 - Pf6N2E2_3756 0.45 +0.8 4,377,577 - Pf6N2E2_3756 0.45 +1.2
Or see this region's nucleotide sequence