Experiment: octanoate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2972 and Pf6N2E2_2973 are separated by 17 nucleotides Pf6N2E2_2973 and Pf6N2E2_2974 are separated by 214 nucleotides
Pf6N2E2_2972 - Prolyl-tRNA synthetase (EC 6.1.1.15), bacterial type, at 3,462,539 to 3,464,254
_2972
Pf6N2E2_2973 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 3,464,272 to 3,465,225
_2973
Pf6N2E2_2974 - DNA polymerase IV (EC 2.7.7.7), at 3,465,440 to 3,466,612
_2974
Position (kb)
3464
3465
3466 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3464.263 kb on - strand at 3464.291 kb on + strand at 3464.291 kb on + strand at 3464.291 kb on + strand at 3464.291 kb on + strand at 3464.292 kb on - strand at 3464.292 kb on - strand at 3464.292 kb on - strand at 3464.292 kb on - strand at 3464.515 kb on + strand, within Pf6N2E2_2973 at 3464.525 kb on - strand, within Pf6N2E2_2973 at 3464.555 kb on - strand, within Pf6N2E2_2973 at 3464.665 kb on + strand, within Pf6N2E2_2973 at 3464.665 kb on + strand, within Pf6N2E2_2973 at 3464.665 kb on + strand, within Pf6N2E2_2973 at 3464.665 kb on + strand, within Pf6N2E2_2973 at 3464.666 kb on - strand, within Pf6N2E2_2973 at 3464.666 kb on - strand, within Pf6N2E2_2973 at 3464.666 kb on - strand, within Pf6N2E2_2973 at 3464.666 kb on - strand, within Pf6N2E2_2973 at 3465.023 kb on - strand, within Pf6N2E2_2973 at 3465.023 kb on - strand, within Pf6N2E2_2973 at 3465.314 kb on + strand at 3465.315 kb on - strand at 3465.315 kb on - strand at 3465.315 kb on - strand at 3465.316 kb on + strand at 3465.316 kb on + strand at 3465.316 kb on + strand at 3465.316 kb on + strand at 3465.317 kb on - strand at 3465.317 kb on - strand at 3465.317 kb on - strand at 3465.561 kb on + strand, within Pf6N2E2_2974 at 3465.561 kb on + strand, within Pf6N2E2_2974 at 3465.562 kb on - strand, within Pf6N2E2_2974 at 3465.562 kb on - strand, within Pf6N2E2_2974 at 3465.750 kb on + strand, within Pf6N2E2_2974 at 3465.750 kb on + strand, within Pf6N2E2_2974 at 3465.751 kb on - strand, within Pf6N2E2_2974 at 3465.751 kb on - strand, within Pf6N2E2_2974 at 3465.751 kb on - strand, within Pf6N2E2_2974 at 3465.751 kb on - strand, within Pf6N2E2_2974 at 3465.751 kb on - strand, within Pf6N2E2_2974 at 3466.180 kb on - strand, within Pf6N2E2_2974 at 3466.218 kb on + strand, within Pf6N2E2_2974 at 3466.219 kb on - strand, within Pf6N2E2_2974 at 3466.219 kb on - strand, within Pf6N2E2_2974
Per-strain Table
Position Strand Gene LocusTag Fraction octanoate (C) remove 3,464,263 - -2.0 3,464,291 + -0.7 3,464,291 + +0.0 3,464,291 + -2.7 3,464,291 + -1.8 3,464,292 - -0.3 3,464,292 - -0.5 3,464,292 - -3.0 3,464,292 - -1.4 3,464,515 + Pf6N2E2_2973 0.25 -0.6 3,464,525 - Pf6N2E2_2973 0.27 -0.6 3,464,555 - Pf6N2E2_2973 0.30 -1.7 3,464,665 + Pf6N2E2_2973 0.41 -2.7 3,464,665 + Pf6N2E2_2973 0.41 -3.4 3,464,665 + Pf6N2E2_2973 0.41 -1.5 3,464,665 + Pf6N2E2_2973 0.41 +1.6 3,464,666 - Pf6N2E2_2973 0.41 -1.5 3,464,666 - Pf6N2E2_2973 0.41 -2.4 3,464,666 - Pf6N2E2_2973 0.41 -0.9 3,464,666 - Pf6N2E2_2973 0.41 -1.4 3,465,023 - Pf6N2E2_2973 0.79 -2.6 3,465,023 - Pf6N2E2_2973 0.79 -2.2 3,465,314 + +0.7 3,465,315 - +0.3 3,465,315 - +1.8 3,465,315 - -0.0 3,465,316 + -0.2 3,465,316 + -0.0 3,465,316 + -0.0 3,465,316 + -2.5 3,465,317 - -0.2 3,465,317 - +0.6 3,465,317 - +0.0 3,465,561 + Pf6N2E2_2974 0.10 -0.1 3,465,561 + Pf6N2E2_2974 0.10 -0.3 3,465,562 - Pf6N2E2_2974 0.10 +0.4 3,465,562 - Pf6N2E2_2974 0.10 +1.4 3,465,750 + Pf6N2E2_2974 0.26 -1.8 3,465,750 + Pf6N2E2_2974 0.26 +0.8 3,465,751 - Pf6N2E2_2974 0.27 -0.5 3,465,751 - Pf6N2E2_2974 0.27 +1.1 3,465,751 - Pf6N2E2_2974 0.27 +0.6 3,465,751 - Pf6N2E2_2974 0.27 +1.0 3,465,751 - Pf6N2E2_2974 0.27 +0.1 3,466,180 - Pf6N2E2_2974 0.63 +0.6 3,466,218 + Pf6N2E2_2974 0.66 -0.8 3,466,219 - Pf6N2E2_2974 0.66 +1.5 3,466,219 - Pf6N2E2_2974 0.66 +0.5
Or see this region's nucleotide sequence