Experiment: octanoate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2822 and Pf6N2E2_2823 are separated by 261 nucleotides Pf6N2E2_2823 and Pf6N2E2_2824 are separated by 3 nucleotides
Pf6N2E2_2822 - Transcription repressor of multidrug efflux pump acrAB operon, TetR (AcrR) family, at 3,308,633 to 3,309,265
_2822
Pf6N2E2_2823 - Multidrug efflux RND membrane fusion protein MexC, at 3,309,527 to 3,310,684
_2823
Pf6N2E2_2824 - RND efflux system, inner membrane transporter CmeB, at 3,310,688 to 3,313,852
_2824
Position (kb)
3309
3310
3311 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3308.528 kb on - strand at 3308.528 kb on - strand at 3308.550 kb on + strand at 3308.575 kb on + strand at 3308.576 kb on - strand at 3308.607 kb on - strand at 3308.666 kb on - strand at 3308.666 kb on - strand at 3308.739 kb on + strand, within Pf6N2E2_2822 at 3308.739 kb on + strand, within Pf6N2E2_2822 at 3308.739 kb on + strand, within Pf6N2E2_2822 at 3308.740 kb on - strand, within Pf6N2E2_2822 at 3308.920 kb on + strand, within Pf6N2E2_2822 at 3308.920 kb on + strand, within Pf6N2E2_2822 at 3308.920 kb on + strand, within Pf6N2E2_2822 at 3308.920 kb on + strand, within Pf6N2E2_2822 at 3308.920 kb on + strand, within Pf6N2E2_2822 at 3308.920 kb on + strand, within Pf6N2E2_2822 at 3308.921 kb on - strand, within Pf6N2E2_2822 at 3308.921 kb on - strand, within Pf6N2E2_2822 at 3308.921 kb on - strand, within Pf6N2E2_2822 at 3308.921 kb on - strand, within Pf6N2E2_2822 at 3308.939 kb on - strand, within Pf6N2E2_2822 at 3309.002 kb on - strand, within Pf6N2E2_2822 at 3309.002 kb on - strand, within Pf6N2E2_2822 at 3309.120 kb on + strand, within Pf6N2E2_2822 at 3309.120 kb on + strand, within Pf6N2E2_2822 at 3309.120 kb on + strand, within Pf6N2E2_2822 at 3309.120 kb on + strand, within Pf6N2E2_2822 at 3309.121 kb on - strand, within Pf6N2E2_2822 at 3309.121 kb on - strand, within Pf6N2E2_2822 at 3309.271 kb on + strand at 3309.271 kb on + strand at 3309.272 kb on - strand at 3309.304 kb on + strand at 3309.305 kb on - strand at 3309.305 kb on - strand at 3309.315 kb on + strand at 3309.315 kb on + strand at 3309.315 kb on + strand at 3309.315 kb on + strand at 3309.315 kb on + strand at 3309.379 kb on + strand at 3309.379 kb on + strand at 3309.379 kb on + strand at 3309.383 kb on + strand at 3309.383 kb on + strand at 3309.384 kb on - strand at 3309.423 kb on + strand at 3309.427 kb on + strand at 3309.433 kb on + strand at 3309.441 kb on - strand at 3309.448 kb on + strand at 3309.448 kb on + strand at 3309.797 kb on + strand, within Pf6N2E2_2823 at 3309.818 kb on + strand, within Pf6N2E2_2823 at 3309.818 kb on + strand, within Pf6N2E2_2823 at 3309.819 kb on - strand, within Pf6N2E2_2823 at 3309.924 kb on - strand, within Pf6N2E2_2823 at 3309.992 kb on + strand, within Pf6N2E2_2823 at 3309.993 kb on - strand, within Pf6N2E2_2823 at 3310.004 kb on + strand, within Pf6N2E2_2823 at 3310.112 kb on + strand, within Pf6N2E2_2823 at 3310.113 kb on - strand, within Pf6N2E2_2823 at 3310.113 kb on - strand, within Pf6N2E2_2823 at 3310.238 kb on + strand, within Pf6N2E2_2823 at 3310.657 kb on + strand at 3310.658 kb on - strand at 3310.916 kb on + strand at 3310.917 kb on - strand at 3310.920 kb on + strand at 3310.920 kb on + strand at 3310.920 kb on + strand at 3310.921 kb on - strand at 3310.934 kb on - strand at 3311.156 kb on + strand, within Pf6N2E2_2824 at 3311.156 kb on + strand, within Pf6N2E2_2824 at 3311.156 kb on + strand, within Pf6N2E2_2824 at 3311.232 kb on - strand, within Pf6N2E2_2824 at 3311.386 kb on + strand, within Pf6N2E2_2824 at 3311.387 kb on - strand, within Pf6N2E2_2824 at 3311.387 kb on - strand, within Pf6N2E2_2824 at 3311.387 kb on - strand, within Pf6N2E2_2824 at 3311.510 kb on + strand, within Pf6N2E2_2824 at 3311.510 kb on + strand, within Pf6N2E2_2824 at 3311.510 kb on + strand, within Pf6N2E2_2824 at 3311.511 kb on - strand, within Pf6N2E2_2824 at 3311.511 kb on - strand, within Pf6N2E2_2824
Per-strain Table
Position Strand Gene LocusTag Fraction octanoate (C) remove 3,308,528 - +3.4 3,308,528 - +1.4 3,308,550 + +0.8 3,308,575 + +0.9 3,308,576 - +0.5 3,308,607 - +0.1 3,308,666 - +1.6 3,308,666 - +1.1 3,308,739 + Pf6N2E2_2822 0.17 +1.4 3,308,739 + Pf6N2E2_2822 0.17 +1.1 3,308,739 + Pf6N2E2_2822 0.17 +1.7 3,308,740 - Pf6N2E2_2822 0.17 +3.4 3,308,920 + Pf6N2E2_2822 0.45 +1.5 3,308,920 + Pf6N2E2_2822 0.45 +1.1 3,308,920 + Pf6N2E2_2822 0.45 +2.0 3,308,920 + Pf6N2E2_2822 0.45 +2.0 3,308,920 + Pf6N2E2_2822 0.45 +1.1 3,308,920 + Pf6N2E2_2822 0.45 +0.6 3,308,921 - Pf6N2E2_2822 0.45 +2.5 3,308,921 - Pf6N2E2_2822 0.45 +1.7 3,308,921 - Pf6N2E2_2822 0.45 +1.7 3,308,921 - Pf6N2E2_2822 0.45 +2.2 3,308,939 - Pf6N2E2_2822 0.48 +1.7 3,309,002 - Pf6N2E2_2822 0.58 +2.1 3,309,002 - Pf6N2E2_2822 0.58 +3.0 3,309,120 + Pf6N2E2_2822 0.77 +1.4 3,309,120 + Pf6N2E2_2822 0.77 +1.1 3,309,120 + Pf6N2E2_2822 0.77 +1.7 3,309,120 + Pf6N2E2_2822 0.77 +2.1 3,309,121 - Pf6N2E2_2822 0.77 +0.8 3,309,121 - Pf6N2E2_2822 0.77 +1.2 3,309,271 + +0.5 3,309,271 + +1.3 3,309,272 - +1.4 3,309,304 + +0.2 3,309,305 - -3.9 3,309,305 - -0.7 3,309,315 + +0.2 3,309,315 + +1.2 3,309,315 + +1.9 3,309,315 + +0.7 3,309,315 + +1.8 3,309,379 + +1.4 3,309,379 + +0.9 3,309,379 + +0.7 3,309,383 + +3.4 3,309,383 + +1.5 3,309,384 - -2.9 3,309,423 + +1.6 3,309,427 + -1.5 3,309,433 + +1.5 3,309,441 - -2.2 3,309,448 + +1.6 3,309,448 + +0.5 3,309,797 + Pf6N2E2_2823 0.23 -2.3 3,309,818 + Pf6N2E2_2823 0.25 -0.8 3,309,818 + Pf6N2E2_2823 0.25 -2.6 3,309,819 - Pf6N2E2_2823 0.25 -0.6 3,309,924 - Pf6N2E2_2823 0.34 -1.2 3,309,992 + Pf6N2E2_2823 0.40 -0.7 3,309,993 - Pf6N2E2_2823 0.40 -3.4 3,310,004 + Pf6N2E2_2823 0.41 -3.3 3,310,112 + Pf6N2E2_2823 0.51 -2.7 3,310,113 - Pf6N2E2_2823 0.51 +0.8 3,310,113 - Pf6N2E2_2823 0.51 -1.7 3,310,238 + Pf6N2E2_2823 0.61 -3.0 3,310,657 + -3.6 3,310,658 - -2.6 3,310,916 + -2.9 3,310,917 - -3.4 3,310,920 + -3.0 3,310,920 + -4.1 3,310,920 + -0.7 3,310,921 - -2.6 3,310,934 - -2.2 3,311,156 + Pf6N2E2_2824 0.15 -0.8 3,311,156 + Pf6N2E2_2824 0.15 -1.7 3,311,156 + Pf6N2E2_2824 0.15 -3.5 3,311,232 - Pf6N2E2_2824 0.17 -0.8 3,311,386 + Pf6N2E2_2824 0.22 -3.7 3,311,387 - Pf6N2E2_2824 0.22 -2.0 3,311,387 - Pf6N2E2_2824 0.22 -2.0 3,311,387 - Pf6N2E2_2824 0.22 -1.1 3,311,510 + Pf6N2E2_2824 0.26 -2.7 3,311,510 + Pf6N2E2_2824 0.26 -2.7 3,311,510 + Pf6N2E2_2824 0.26 -1.0 3,311,511 - Pf6N2E2_2824 0.26 -2.6 3,311,511 - Pf6N2E2_2824 0.26 -1.5
Or see this region's nucleotide sequence