Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02480 and MMP_RS02485 are separated by 2 nucleotides MMP_RS02485 and MMP_RS02490 overlap by 14 nucleotides MMP_RS02490 and MMP_RS02495 are separated by 11 nucleotides MMP_RS02495 and MMP_RS02500 are separated by 1 nucleotides MMP_RS02500 and MMP_RS02505 are separated by 3 nucleotides MMP_RS02505 and MMP_RS02510 overlap by 17 nucleotides
MMP_RS02480: MMP_RS02480 - hypothetical protein, at 464,479 to 464,997
_RS02480
MMP_RS02485: MMP_RS02485 - DUF2080 family transposase-associated protein, at 465,000 to 465,224
_RS02485
MMP_RS02490: MMP_RS02490 - hypothetical protein, at 465,211 to 465,405
_RS02490
MMP_RS02495: MMP_RS02495 - hypothetical protein, at 465,417 to 465,800
_RS02495
MMP_RS02500: MMP_RS02500 - minichromosome maintenance protein MCM, at 465,802 to 467,826
_RS02500
MMP_RS02505: MMP_RS02505 - DUF2540 domain-containing protein, at 467,830 to 468,102
_RS02505
MMP_RS02510: MMP_RS02510 - site-specific integrase, at 468,086 to 469,072
_RS02510
Position (kb) 465
466
467
468 Strain fitness (log2 ratio) -1
0
1 at 464.825 kb on - strand, within MMP_RS02480 at 465.306 kb on - strand, within MMP_RS02490 at 465.306 kb on - strand, within MMP_RS02490 at 465.307 kb on + strand, within MMP_RS02490 at 465.432 kb on - strand at 465.679 kb on + strand, within MMP_RS02495 at 465.682 kb on - strand, within MMP_RS02495 at 465.851 kb on - strand at 466.888 kb on + strand, within MMP_RS02500 at 467.298 kb on + strand, within MMP_RS02500 at 467.326 kb on - strand, within MMP_RS02500 at 467.326 kb on - strand, within MMP_RS02500 at 467.327 kb on + strand, within MMP_RS02500 at 467.330 kb on - strand, within MMP_RS02500 at 467.331 kb on + strand, within MMP_RS02500 at 467.331 kb on + strand, within MMP_RS02500 at 467.527 kb on - strand, within MMP_RS02500 at 467.560 kb on - strand, within MMP_RS02500 at 468.093 kb on - strand at 468.230 kb on + strand, within MMP_RS02510 at 468.294 kb on + strand, within MMP_RS02510 at 468.395 kb on + strand, within MMP_RS02510 at 468.485 kb on - strand, within MMP_RS02510 at 468.486 kb on + strand, within MMP_RS02510 at 468.570 kb on - strand, within MMP_RS02510 at 468.570 kb on - strand, within MMP_RS02510 at 468.747 kb on + strand, within MMP_RS02510
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE remove 464,825 - MMP_RS02480 0.67 +0.1 465,306 - MMP_RS02490 0.49 -0.1 465,306 - MMP_RS02490 0.49 +0.4 465,307 + MMP_RS02490 0.49 +0.1 465,432 - +0.1 465,679 + MMP_RS02495 0.68 -0.2 465,682 - MMP_RS02495 0.69 +0.5 465,851 - -0.4 466,888 + MMP_RS02500 0.54 -0.5 467,298 + MMP_RS02500 0.74 +0.7 467,326 - MMP_RS02500 0.75 +0.5 467,326 - MMP_RS02500 0.75 -0.1 467,327 + MMP_RS02500 0.75 +0.5 467,330 - MMP_RS02500 0.75 +1.2 467,331 + MMP_RS02500 0.76 +0.0 467,331 + MMP_RS02500 0.76 +0.2 467,527 - MMP_RS02500 0.85 +0.2 467,560 - MMP_RS02500 0.87 +0.4 468,093 - +0.4 468,230 + MMP_RS02510 0.15 +0.5 468,294 + MMP_RS02510 0.21 +0.4 468,395 + MMP_RS02510 0.31 -0.1 468,485 - MMP_RS02510 0.40 +0.1 468,486 + MMP_RS02510 0.41 -0.2 468,570 - MMP_RS02510 0.49 +0.2 468,570 - MMP_RS02510 0.49 -0.0 468,747 + MMP_RS02510 0.67 +0.7
Or see this region's nucleotide sequence