Experiment: R2A with A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS25405 and CA265_RS25410 are separated by 6 nucleotides CA265_RS25410 and CA265_RS25415 overlap by 23 nucleotides CA265_RS25415 and CA265_RS25420 are separated by 161 nucleotides
CA265_RS25405: CA265_RS25405 - ferrochelatase, at 6,169,712 to 6,170,722
_RS25405
CA265_RS25410: CA265_RS25410 - gluconate kinase, at 6,170,729 to 6,171,235
_RS25410
CA265_RS25415: CA265_RS25415 - 3-deoxy-D-manno-octulosonic acid transferase, at 6,171,213 to 6,172,445
_RS25415
CA265_RS25420: CA265_RS25420 - UDP-glucose 6-dehydrogenase, at 6,172,607 to 6,173,920
_RS25420
Position (kb)
6171
6172
6173 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 6170.717 kb on - strand at 6170.726 kb on - strand at 6170.726 kb on - strand at 6170.726 kb on - strand at 6170.728 kb on + strand at 6170.770 kb on + strand at 6170.805 kb on + strand, within CA265_RS25410 at 6170.831 kb on - strand, within CA265_RS25410 at 6170.950 kb on + strand, within CA265_RS25410 at 6170.950 kb on + strand, within CA265_RS25410 at 6170.959 kb on + strand, within CA265_RS25410 at 6170.959 kb on + strand, within CA265_RS25410 at 6170.960 kb on - strand, within CA265_RS25410 at 6170.963 kb on - strand, within CA265_RS25410 at 6170.963 kb on - strand, within CA265_RS25410 at 6171.004 kb on - strand, within CA265_RS25410 at 6171.004 kb on - strand, within CA265_RS25410 at 6171.013 kb on + strand, within CA265_RS25410 at 6171.092 kb on + strand, within CA265_RS25410 at 6171.093 kb on - strand, within CA265_RS25410 at 6171.095 kb on - strand, within CA265_RS25410 at 6171.095 kb on - strand, within CA265_RS25410 at 6171.103 kb on + strand, within CA265_RS25410 at 6171.280 kb on + strand at 6171.317 kb on + strand at 6171.317 kb on + strand at 6171.492 kb on + strand, within CA265_RS25415 at 6171.493 kb on - strand, within CA265_RS25415 at 6171.501 kb on + strand, within CA265_RS25415 at 6171.519 kb on + strand, within CA265_RS25415 at 6171.617 kb on + strand, within CA265_RS25415 at 6171.680 kb on + strand, within CA265_RS25415 at 6171.681 kb on - strand, within CA265_RS25415 at 6171.744 kb on - strand, within CA265_RS25415 at 6171.757 kb on - strand, within CA265_RS25415 at 6171.776 kb on + strand, within CA265_RS25415 at 6171.864 kb on - strand, within CA265_RS25415 at 6171.939 kb on - strand, within CA265_RS25415 at 6171.959 kb on - strand, within CA265_RS25415 at 6172.043 kb on - strand, within CA265_RS25415 at 6172.060 kb on + strand, within CA265_RS25415 at 6172.060 kb on + strand, within CA265_RS25415 at 6172.092 kb on - strand, within CA265_RS25415 at 6172.092 kb on - strand, within CA265_RS25415 at 6172.092 kb on - strand, within CA265_RS25415 at 6172.100 kb on + strand, within CA265_RS25415 at 6172.294 kb on - strand, within CA265_RS25415 at 6172.336 kb on + strand at 6172.336 kb on + strand at 6172.337 kb on - strand at 6172.337 kb on - strand at 6172.340 kb on + strand at 6172.383 kb on + strand at 6172.452 kb on - strand at 6172.601 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.02 mg/ml remove 6,170,717 - -1.2 6,170,726 - +0.8 6,170,726 - +0.1 6,170,726 - +0.4 6,170,728 + +0.0 6,170,770 + -0.3 6,170,805 + CA265_RS25410 0.15 +0.1 6,170,831 - CA265_RS25410 0.20 -0.1 6,170,950 + CA265_RS25410 0.44 -0.7 6,170,950 + CA265_RS25410 0.44 -0.2 6,170,959 + CA265_RS25410 0.45 +0.3 6,170,959 + CA265_RS25410 0.45 +0.9 6,170,960 - CA265_RS25410 0.46 +0.6 6,170,963 - CA265_RS25410 0.46 -0.1 6,170,963 - CA265_RS25410 0.46 -1.6 6,171,004 - CA265_RS25410 0.54 +0.4 6,171,004 - CA265_RS25410 0.54 -0.9 6,171,013 + CA265_RS25410 0.56 +0.1 6,171,092 + CA265_RS25410 0.72 +0.1 6,171,093 - CA265_RS25410 0.72 +0.8 6,171,095 - CA265_RS25410 0.72 -0.1 6,171,095 - CA265_RS25410 0.72 -0.2 6,171,103 + CA265_RS25410 0.74 +1.1 6,171,280 + -1.7 6,171,317 + -1.3 6,171,317 + -1.8 6,171,492 + CA265_RS25415 0.23 -2.6 6,171,493 - CA265_RS25415 0.23 +0.0 6,171,501 + CA265_RS25415 0.23 -1.0 6,171,519 + CA265_RS25415 0.25 -1.5 6,171,617 + CA265_RS25415 0.33 -0.9 6,171,680 + CA265_RS25415 0.38 -1.1 6,171,681 - CA265_RS25415 0.38 -1.8 6,171,744 - CA265_RS25415 0.43 -1.1 6,171,757 - CA265_RS25415 0.44 -0.1 6,171,776 + CA265_RS25415 0.46 -1.0 6,171,864 - CA265_RS25415 0.53 -0.6 6,171,939 - CA265_RS25415 0.59 -2.4 6,171,959 - CA265_RS25415 0.61 -3.4 6,172,043 - CA265_RS25415 0.67 -1.0 6,172,060 + CA265_RS25415 0.69 -1.3 6,172,060 + CA265_RS25415 0.69 -2.3 6,172,092 - CA265_RS25415 0.71 -1.2 6,172,092 - CA265_RS25415 0.71 -2.8 6,172,092 - CA265_RS25415 0.71 -1.7 6,172,100 + CA265_RS25415 0.72 +0.1 6,172,294 - CA265_RS25415 0.88 -1.6 6,172,336 + -0.8 6,172,336 + +0.5 6,172,337 - -2.0 6,172,337 - -2.4 6,172,340 + -1.8 6,172,383 + -1.3 6,172,452 - +0.1 6,172,601 + -0.2
Or see this region's nucleotide sequence