Experiment: R2A with A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS24245 and CA265_RS24250 are separated by 389 nucleotides CA265_RS24250 and CA265_RS24255 are separated by 140 nucleotides CA265_RS24255 and CA265_RS24260 are separated by 144 nucleotides CA265_RS24260 and CA265_RS24265 are separated by 33 nucleotides CA265_RS24265 and CA265_RS24270 are separated by 303 nucleotides
CA265_RS24245: CA265_RS24245 - hypothetical protein, at 5,904,380 to 5,905,066
_RS24245
CA265_RS24250: CA265_RS24250 - cytochrome b5, at 5,905,456 to 5,905,698
_RS24250
CA265_RS24255: CA265_RS24255 - sugar kinase, at 5,905,839 to 5,906,759
_RS24255
CA265_RS24260: CA265_RS24260 - tRNA-Asn, at 5,906,904 to 5,906,980
_RS24260
CA265_RS24265: CA265_RS24265 - hypothetical protein, at 5,907,014 to 5,907,385
_RS24265
CA265_RS24270: CA265_RS24270 - hypothetical protein, at 5,907,689 to 5,907,898
_RS24270
Position (kb) 5905
5906
5907 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5904.866 kb on + strand, within CA265_RS24245 at 5904.890 kb on + strand, within CA265_RS24245 at 5904.893 kb on - strand, within CA265_RS24245 at 5904.916 kb on + strand, within CA265_RS24245 at 5904.932 kb on + strand, within CA265_RS24245 at 5904.967 kb on + strand, within CA265_RS24245 at 5904.967 kb on + strand, within CA265_RS24245 at 5904.967 kb on + strand, within CA265_RS24245 at 5905.019 kb on - strand at 5905.022 kb on + strand at 5905.129 kb on + strand at 5905.264 kb on + strand at 5905.287 kb on + strand at 5905.352 kb on - strand at 5905.352 kb on - strand at 5905.381 kb on - strand at 5905.383 kb on - strand at 5905.383 kb on - strand at 5905.388 kb on + strand at 5905.391 kb on - strand at 5905.453 kb on + strand at 5905.464 kb on - strand at 5905.474 kb on - strand at 5905.520 kb on + strand, within CA265_RS24250 at 5905.521 kb on - strand, within CA265_RS24250 at 5905.679 kb on + strand at 5905.711 kb on + strand at 5905.732 kb on + strand at 5905.737 kb on + strand at 5905.840 kb on + strand at 5905.840 kb on + strand at 5905.841 kb on - strand at 5905.864 kb on - strand at 5905.864 kb on - strand at 5906.074 kb on - strand, within CA265_RS24255 at 5906.074 kb on - strand, within CA265_RS24255 at 5906.165 kb on + strand, within CA265_RS24255 at 5906.253 kb on + strand, within CA265_RS24255 at 5906.254 kb on - strand, within CA265_RS24255 at 5906.267 kb on + strand, within CA265_RS24255 at 5906.294 kb on + strand, within CA265_RS24255 at 5906.295 kb on - strand, within CA265_RS24255 at 5906.405 kb on - strand, within CA265_RS24255 at 5906.420 kb on - strand, within CA265_RS24255 at 5906.541 kb on - strand, within CA265_RS24255 at 5906.636 kb on + strand, within CA265_RS24255 at 5906.637 kb on - strand, within CA265_RS24255 at 5906.641 kb on + strand, within CA265_RS24255 at 5906.647 kb on + strand, within CA265_RS24255 at 5906.734 kb on - strand at 5906.739 kb on - strand at 5906.807 kb on + strand at 5906.811 kb on + strand at 5906.854 kb on + strand at 5906.870 kb on + strand at 5906.902 kb on - strand at 5907.096 kb on - strand, within CA265_RS24265 at 5907.128 kb on + strand, within CA265_RS24265 at 5907.128 kb on + strand, within CA265_RS24265 at 5907.131 kb on - strand, within CA265_RS24265 at 5907.172 kb on + strand, within CA265_RS24265 at 5907.176 kb on - strand, within CA265_RS24265 at 5907.234 kb on - strand, within CA265_RS24265 at 5907.249 kb on + strand, within CA265_RS24265 at 5907.320 kb on - strand, within CA265_RS24265 at 5907.384 kb on - strand at 5907.591 kb on + strand at 5907.690 kb on + strand at 5907.732 kb on - strand, within CA265_RS24270 at 5907.756 kb on - strand, within CA265_RS24270 at 5907.759 kb on + strand, within CA265_RS24270
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.02 mg/ml remove 5,904,866 + CA265_RS24245 0.71 -0.8 5,904,890 + CA265_RS24245 0.74 -0.3 5,904,893 - CA265_RS24245 0.75 +0.3 5,904,916 + CA265_RS24245 0.78 -0.5 5,904,932 + CA265_RS24245 0.80 -2.8 5,904,967 + CA265_RS24245 0.85 +0.5 5,904,967 + CA265_RS24245 0.85 -1.1 5,904,967 + CA265_RS24245 0.85 +2.9 5,905,019 - +0.4 5,905,022 + -1.2 5,905,129 + +0.5 5,905,264 + -0.0 5,905,287 + +0.6 5,905,352 - -0.1 5,905,352 - +0.1 5,905,381 - +0.3 5,905,383 - -0.1 5,905,383 - -0.6 5,905,388 + +0.0 5,905,391 - -0.3 5,905,453 + +1.6 5,905,464 - +0.3 5,905,474 - +0.2 5,905,520 + CA265_RS24250 0.26 +0.4 5,905,521 - CA265_RS24250 0.27 -1.1 5,905,679 + +0.3 5,905,711 + -1.7 5,905,732 + +0.0 5,905,737 + -0.0 5,905,840 + -0.6 5,905,840 + +0.2 5,905,841 - -0.3 5,905,864 - -0.3 5,905,864 - +0.1 5,906,074 - CA265_RS24255 0.26 -2.0 5,906,074 - CA265_RS24255 0.26 -1.3 5,906,165 + CA265_RS24255 0.35 +0.3 5,906,253 + CA265_RS24255 0.45 +0.0 5,906,254 - CA265_RS24255 0.45 -1.7 5,906,267 + CA265_RS24255 0.46 -0.1 5,906,294 + CA265_RS24255 0.49 +0.6 5,906,295 - CA265_RS24255 0.50 -1.1 5,906,405 - CA265_RS24255 0.61 -0.1 5,906,420 - CA265_RS24255 0.63 -0.7 5,906,541 - CA265_RS24255 0.76 -2.5 5,906,636 + CA265_RS24255 0.87 -0.6 5,906,637 - CA265_RS24255 0.87 +0.6 5,906,641 + CA265_RS24255 0.87 +0.9 5,906,647 + CA265_RS24255 0.88 +0.1 5,906,734 - -0.5 5,906,739 - +0.3 5,906,807 + +0.6 5,906,811 + -1.2 5,906,854 + +0.1 5,906,870 + +0.0 5,906,902 - -0.7 5,907,096 - CA265_RS24265 0.22 -0.8 5,907,128 + CA265_RS24265 0.31 +0.6 5,907,128 + CA265_RS24265 0.31 +1.5 5,907,131 - CA265_RS24265 0.31 +0.1 5,907,172 + CA265_RS24265 0.42 -0.1 5,907,176 - CA265_RS24265 0.44 -0.1 5,907,234 - CA265_RS24265 0.59 -0.0 5,907,249 + CA265_RS24265 0.63 -0.0 5,907,320 - CA265_RS24265 0.82 -0.1 5,907,384 - -0.2 5,907,591 + +0.6 5,907,690 + +0.7 5,907,732 - CA265_RS24270 0.20 -0.6 5,907,756 - CA265_RS24270 0.32 +0.1 5,907,759 + CA265_RS24270 0.33 -0.1
Or see this region's nucleotide sequence