Experiment: R2A with A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS22680 and CA265_RS22685 are separated by 52 nucleotides CA265_RS22685 and CA265_RS22690 are separated by 145 nucleotides CA265_RS22690 and CA265_RS22695 are separated by 21 nucleotides CA265_RS22695 and CA265_RS22700 are separated by 143 nucleotides
CA265_RS22680: CA265_RS22680 - A/G-specific adenine glycosylase, at 5,553,293 to 5,554,357
_RS22680
CA265_RS22685: CA265_RS22685 - single-stranded DNA-binding protein, at 5,554,410 to 5,554,847
_RS22685
CA265_RS22690: CA265_RS22690 - hypothetical protein, at 5,554,993 to 5,556,432
_RS22690
CA265_RS22695: CA265_RS22695 - starch synthase, at 5,556,454 to 5,557,284
_RS22695
CA265_RS22700: CA265_RS22700 - pantoate--beta-alanine ligase, at 5,557,428 to 5,558,273
_RS22700
Position (kb) 5554
5555
5556
5557 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5554.041 kb on + strand, within CA265_RS22680 at 5554.042 kb on - strand, within CA265_RS22680 at 5554.042 kb on - strand, within CA265_RS22680 at 5554.042 kb on - strand, within CA265_RS22680 at 5554.042 kb on - strand, within CA265_RS22680 at 5554.054 kb on - strand, within CA265_RS22680 at 5554.054 kb on - strand, within CA265_RS22680 at 5554.089 kb on - strand, within CA265_RS22680 at 5554.123 kb on - strand, within CA265_RS22680 at 5554.159 kb on + strand, within CA265_RS22680 at 5554.159 kb on + strand, within CA265_RS22680 at 5554.179 kb on + strand, within CA265_RS22680 at 5554.180 kb on - strand, within CA265_RS22680 at 5554.183 kb on - strand, within CA265_RS22680 at 5554.231 kb on - strand, within CA265_RS22680 at 5554.276 kb on + strand at 5554.282 kb on - strand at 5554.336 kb on + strand at 5554.344 kb on + strand at 5554.345 kb on - strand at 5554.345 kb on - strand at 5554.351 kb on + strand at 5554.351 kb on + strand at 5554.356 kb on - strand at 5554.356 kb on - strand at 5554.409 kb on + strand at 5554.431 kb on - strand at 5554.569 kb on - strand, within CA265_RS22685 at 5554.598 kb on - strand, within CA265_RS22685 at 5554.634 kb on + strand, within CA265_RS22685 at 5554.761 kb on - strand, within CA265_RS22685 at 5554.861 kb on + strand at 5554.862 kb on - strand at 5554.866 kb on - strand at 5554.870 kb on + strand at 5554.871 kb on - strand at 5554.871 kb on - strand at 5554.876 kb on - strand at 5554.998 kb on + strand at 5555.005 kb on - strand at 5555.020 kb on + strand at 5555.058 kb on + strand at 5555.058 kb on + strand at 5555.108 kb on + strand at 5555.109 kb on - strand at 5555.115 kb on + strand at 5555.115 kb on + strand at 5555.115 kb on + strand at 5555.116 kb on - strand at 5555.267 kb on + strand, within CA265_RS22690 at 5555.268 kb on - strand, within CA265_RS22690 at 5555.298 kb on + strand, within CA265_RS22690 at 5555.310 kb on - strand, within CA265_RS22690 at 5555.334 kb on + strand, within CA265_RS22690 at 5555.334 kb on + strand, within CA265_RS22690 at 5555.335 kb on - strand, within CA265_RS22690 at 5555.343 kb on - strand, within CA265_RS22690 at 5555.392 kb on - strand, within CA265_RS22690 at 5555.406 kb on - strand, within CA265_RS22690 at 5555.406 kb on - strand, within CA265_RS22690 at 5555.471 kb on - strand, within CA265_RS22690 at 5555.475 kb on - strand, within CA265_RS22690 at 5555.479 kb on + strand, within CA265_RS22690 at 5555.484 kb on + strand, within CA265_RS22690 at 5555.485 kb on - strand, within CA265_RS22690 at 5555.485 kb on - strand, within CA265_RS22690 at 5555.485 kb on - strand, within CA265_RS22690 at 5555.486 kb on + strand, within CA265_RS22690 at 5555.504 kb on - strand, within CA265_RS22690 at 5555.549 kb on + strand, within CA265_RS22690 at 5555.585 kb on - strand, within CA265_RS22690 at 5555.635 kb on + strand, within CA265_RS22690 at 5555.693 kb on + strand, within CA265_RS22690 at 5555.743 kb on - strand, within CA265_RS22690 at 5555.768 kb on + strand, within CA265_RS22690 at 5555.768 kb on + strand, within CA265_RS22690 at 5555.769 kb on - strand, within CA265_RS22690 at 5555.820 kb on + strand, within CA265_RS22690 at 5555.848 kb on - strand, within CA265_RS22690 at 5555.885 kb on - strand, within CA265_RS22690 at 5555.898 kb on + strand, within CA265_RS22690 at 5555.920 kb on - strand, within CA265_RS22690 at 5555.961 kb on + strand, within CA265_RS22690 at 5555.962 kb on - strand, within CA265_RS22690 at 5555.968 kb on - strand, within CA265_RS22690 at 5555.971 kb on + strand, within CA265_RS22690 at 5555.972 kb on - strand, within CA265_RS22690 at 5555.980 kb on - strand, within CA265_RS22690 at 5555.996 kb on - strand, within CA265_RS22690 at 5556.054 kb on - strand, within CA265_RS22690 at 5556.102 kb on + strand, within CA265_RS22690 at 5556.133 kb on + strand, within CA265_RS22690 at 5556.134 kb on - strand, within CA265_RS22690 at 5556.224 kb on - strand, within CA265_RS22690 at 5556.224 kb on - strand, within CA265_RS22690 at 5556.228 kb on + strand, within CA265_RS22690 at 5556.228 kb on + strand, within CA265_RS22690 at 5556.231 kb on - strand, within CA265_RS22690 at 5556.331 kb on - strand at 5556.410 kb on - strand at 5556.410 kb on - strand at 5556.410 kb on - strand at 5556.447 kb on + strand at 5556.455 kb on + strand at 5557.389 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.02 mg/ml remove 5,554,041 + CA265_RS22680 0.70 -0.2 5,554,042 - CA265_RS22680 0.70 -1.1 5,554,042 - CA265_RS22680 0.70 -1.0 5,554,042 - CA265_RS22680 0.70 -0.5 5,554,042 - CA265_RS22680 0.70 +0.1 5,554,054 - CA265_RS22680 0.71 -0.4 5,554,054 - CA265_RS22680 0.71 +0.4 5,554,089 - CA265_RS22680 0.75 +0.2 5,554,123 - CA265_RS22680 0.78 +0.1 5,554,159 + CA265_RS22680 0.81 -0.1 5,554,159 + CA265_RS22680 0.81 +0.6 5,554,179 + CA265_RS22680 0.83 -0.0 5,554,180 - CA265_RS22680 0.83 +0.2 5,554,183 - CA265_RS22680 0.84 +1.8 5,554,231 - CA265_RS22680 0.88 +0.2 5,554,276 + +0.7 5,554,282 - -0.3 5,554,336 + +0.0 5,554,344 + +1.7 5,554,345 - -0.6 5,554,345 - -0.6 5,554,351 + +0.6 5,554,351 + -0.6 5,554,356 - -0.1 5,554,356 - -1.0 5,554,409 + -0.6 5,554,431 - +0.2 5,554,569 - CA265_RS22685 0.36 -0.6 5,554,598 - CA265_RS22685 0.43 +1.0 5,554,634 + CA265_RS22685 0.51 +1.0 5,554,761 - CA265_RS22685 0.80 -1.6 5,554,861 + -0.2 5,554,862 - +0.8 5,554,866 - +0.4 5,554,870 + +0.8 5,554,871 - +1.3 5,554,871 - -0.5 5,554,876 - -0.3 5,554,998 + +0.5 5,555,005 - -1.5 5,555,020 + -0.8 5,555,058 + -1.3 5,555,058 + -0.1 5,555,108 + -1.8 5,555,109 - +2.0 5,555,115 + -1.9 5,555,115 + -0.1 5,555,115 + -1.3 5,555,116 - -1.1 5,555,267 + CA265_RS22690 0.19 -1.3 5,555,268 - CA265_RS22690 0.19 +1.5 5,555,298 + CA265_RS22690 0.21 -2.0 5,555,310 - CA265_RS22690 0.22 +0.7 5,555,334 + CA265_RS22690 0.24 -0.4 5,555,334 + CA265_RS22690 0.24 -0.4 5,555,335 - CA265_RS22690 0.24 -0.3 5,555,343 - CA265_RS22690 0.24 +0.1 5,555,392 - CA265_RS22690 0.28 -0.8 5,555,406 - CA265_RS22690 0.29 -2.4 5,555,406 - CA265_RS22690 0.29 +2.0 5,555,471 - CA265_RS22690 0.33 -1.7 5,555,475 - CA265_RS22690 0.33 +0.4 5,555,479 + CA265_RS22690 0.34 -0.5 5,555,484 + CA265_RS22690 0.34 -0.2 5,555,485 - CA265_RS22690 0.34 -0.0 5,555,485 - CA265_RS22690 0.34 -0.5 5,555,485 - CA265_RS22690 0.34 +0.2 5,555,486 + CA265_RS22690 0.34 -0.8 5,555,504 - CA265_RS22690 0.35 +0.4 5,555,549 + CA265_RS22690 0.39 -0.8 5,555,585 - CA265_RS22690 0.41 +0.1 5,555,635 + CA265_RS22690 0.45 -1.5 5,555,693 + CA265_RS22690 0.49 +0.0 5,555,743 - CA265_RS22690 0.52 -0.8 5,555,768 + CA265_RS22690 0.54 -0.5 5,555,768 + CA265_RS22690 0.54 -1.9 5,555,769 - CA265_RS22690 0.54 -1.9 5,555,820 + CA265_RS22690 0.57 +1.2 5,555,848 - CA265_RS22690 0.59 +2.0 5,555,885 - CA265_RS22690 0.62 -1.0 5,555,898 + CA265_RS22690 0.63 -0.0 5,555,920 - CA265_RS22690 0.64 -0.5 5,555,961 + CA265_RS22690 0.67 -0.8 5,555,962 - CA265_RS22690 0.67 -0.6 5,555,968 - CA265_RS22690 0.68 -1.6 5,555,971 + CA265_RS22690 0.68 +0.2 5,555,972 - CA265_RS22690 0.68 -0.3 5,555,980 - CA265_RS22690 0.69 -0.8 5,555,996 - CA265_RS22690 0.70 -0.2 5,556,054 - CA265_RS22690 0.74 -2.3 5,556,102 + CA265_RS22690 0.77 -1.3 5,556,133 + CA265_RS22690 0.79 -0.7 5,556,134 - CA265_RS22690 0.79 -0.4 5,556,224 - CA265_RS22690 0.85 -0.8 5,556,224 - CA265_RS22690 0.85 -1.0 5,556,228 + CA265_RS22690 0.86 -1.4 5,556,228 + CA265_RS22690 0.86 -0.9 5,556,231 - CA265_RS22690 0.86 -0.6 5,556,331 - -0.7 5,556,410 - -0.9 5,556,410 - -1.0 5,556,410 - -0.6 5,556,447 + +1.0 5,556,455 + -1.4 5,557,389 + -0.1
Or see this region's nucleotide sequence