Experiment: R2A with A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS22305 and CA265_RS22310 are separated by 121 nucleotides CA265_RS22310 and CA265_RS22315 are separated by 192 nucleotides CA265_RS22315 and CA265_RS22320 are separated by 307 nucleotides CA265_RS22320 and CA265_RS22325 are separated by 61 nucleotides
CA265_RS22305: CA265_RS22305 - hypothetical protein, at 5,478,777 to 5,479,124
_RS22305
CA265_RS22310: CA265_RS22310 - superoxide dismutase, at 5,479,246 to 5,479,776
_RS22310
CA265_RS22315: CA265_RS22315 - hypothetical protein, at 5,479,969 to 5,480,502
_RS22315
CA265_RS22320: CA265_RS22320 - hypothetical protein, at 5,480,810 to 5,481,016
_RS22320
CA265_RS22325: CA265_RS22325 - secretion protein HlyD, at 5,481,078 to 5,482,238
_RS22325
Position (kb)
5479
5480
5481 Strain fitness (log2 ratio)
-2
-1
0
1 at 5478.982 kb on - strand, within CA265_RS22305 at 5479.124 kb on + strand at 5479.125 kb on - strand at 5479.125 kb on - strand at 5479.125 kb on - strand at 5479.128 kb on + strand at 5479.128 kb on + strand at 5479.130 kb on + strand at 5479.143 kb on - strand at 5479.232 kb on + strand at 5479.268 kb on + strand at 5479.272 kb on + strand at 5479.274 kb on + strand at 5479.449 kb on + strand, within CA265_RS22310 at 5479.449 kb on + strand, within CA265_RS22310 at 5479.450 kb on - strand, within CA265_RS22310 at 5479.450 kb on - strand, within CA265_RS22310 at 5479.450 kb on - strand, within CA265_RS22310 at 5479.575 kb on + strand at 5479.592 kb on + strand, within CA265_RS22310 at 5479.593 kb on - strand, within CA265_RS22310 at 5479.611 kb on + strand at 5479.689 kb on - strand, within CA265_RS22310 at 5479.689 kb on - strand, within CA265_RS22310 at 5479.708 kb on + strand, within CA265_RS22310 at 5479.710 kb on + strand, within CA265_RS22310 at 5479.711 kb on - strand, within CA265_RS22310 at 5479.774 kb on + strand at 5479.779 kb on + strand at 5479.783 kb on + strand at 5479.803 kb on + strand at 5479.803 kb on + strand at 5479.979 kb on + strand at 5480.040 kb on - strand, within CA265_RS22315 at 5480.072 kb on + strand, within CA265_RS22315 at 5480.075 kb on + strand, within CA265_RS22315 at 5480.080 kb on - strand, within CA265_RS22315 at 5480.161 kb on - strand, within CA265_RS22315 at 5480.161 kb on - strand, within CA265_RS22315 at 5480.215 kb on + strand, within CA265_RS22315 at 5480.241 kb on - strand, within CA265_RS22315 at 5480.253 kb on + strand, within CA265_RS22315 at 5480.331 kb on + strand, within CA265_RS22315 at 5480.345 kb on - strand, within CA265_RS22315 at 5480.402 kb on + strand, within CA265_RS22315 at 5480.438 kb on - strand, within CA265_RS22315 at 5480.457 kb on + strand at 5480.458 kb on - strand at 5480.588 kb on - strand at 5480.660 kb on - strand at 5480.660 kb on - strand at 5480.708 kb on + strand at 5480.709 kb on - strand at 5480.893 kb on + strand, within CA265_RS22320 at 5480.894 kb on - strand, within CA265_RS22320 at 5480.899 kb on + strand, within CA265_RS22320 at 5480.899 kb on + strand, within CA265_RS22320 at 5480.900 kb on - strand, within CA265_RS22320 at 5481.029 kb on - strand at 5481.165 kb on + strand at 5481.165 kb on + strand at 5481.219 kb on - strand, within CA265_RS22325 at 5481.223 kb on + strand, within CA265_RS22325 at 5481.231 kb on - strand, within CA265_RS22325 at 5481.233 kb on + strand, within CA265_RS22325 at 5481.234 kb on - strand, within CA265_RS22325 at 5481.235 kb on + strand, within CA265_RS22325 at 5481.235 kb on + strand, within CA265_RS22325 at 5481.281 kb on + strand, within CA265_RS22325 at 5481.307 kb on + strand, within CA265_RS22325 at 5481.329 kb on + strand, within CA265_RS22325 at 5481.329 kb on + strand, within CA265_RS22325 at 5481.330 kb on - strand, within CA265_RS22325
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.02 mg/ml remove 5,478,982 - CA265_RS22305 0.59 -0.0 5,479,124 + -0.0 5,479,125 - -0.7 5,479,125 - -0.4 5,479,125 - -0.4 5,479,128 + -0.3 5,479,128 + -0.1 5,479,130 + -0.4 5,479,143 - -0.1 5,479,232 + +0.3 5,479,268 + +0.2 5,479,272 + +0.2 5,479,274 + -1.0 5,479,449 + CA265_RS22310 0.38 +0.2 5,479,449 + CA265_RS22310 0.38 +0.0 5,479,450 - CA265_RS22310 0.38 +1.1 5,479,450 - CA265_RS22310 0.38 -0.5 5,479,450 - CA265_RS22310 0.38 +0.1 5,479,575 + +1.0 5,479,592 + CA265_RS22310 0.65 -0.7 5,479,593 - CA265_RS22310 0.65 +0.1 5,479,611 + +1.0 5,479,689 - CA265_RS22310 0.83 +0.3 5,479,689 - CA265_RS22310 0.83 -0.6 5,479,708 + CA265_RS22310 0.87 -0.8 5,479,710 + CA265_RS22310 0.87 +0.7 5,479,711 - CA265_RS22310 0.88 +0.6 5,479,774 + -1.3 5,479,779 + -0.6 5,479,783 + -1.6 5,479,803 + +0.0 5,479,803 + +0.4 5,479,979 + -0.5 5,480,040 - CA265_RS22315 0.13 -1.0 5,480,072 + CA265_RS22315 0.19 -0.8 5,480,075 + CA265_RS22315 0.20 -1.2 5,480,080 - CA265_RS22315 0.21 +0.4 5,480,161 - CA265_RS22315 0.36 -0.6 5,480,161 - CA265_RS22315 0.36 -0.4 5,480,215 + CA265_RS22315 0.46 -1.3 5,480,241 - CA265_RS22315 0.51 +0.1 5,480,253 + CA265_RS22315 0.53 +0.5 5,480,331 + CA265_RS22315 0.68 -0.9 5,480,345 - CA265_RS22315 0.70 -0.6 5,480,402 + CA265_RS22315 0.81 -0.6 5,480,438 - CA265_RS22315 0.88 -0.0 5,480,457 + -0.6 5,480,458 - -0.3 5,480,588 - -0.8 5,480,660 - -0.2 5,480,660 - -0.5 5,480,708 + +0.3 5,480,709 - +0.6 5,480,893 + CA265_RS22320 0.40 +0.3 5,480,894 - CA265_RS22320 0.41 +0.2 5,480,899 + CA265_RS22320 0.43 -2.3 5,480,899 + CA265_RS22320 0.43 -0.5 5,480,900 - CA265_RS22320 0.43 -0.2 5,481,029 - -0.4 5,481,165 + +0.1 5,481,165 + +0.8 5,481,219 - CA265_RS22325 0.12 -0.8 5,481,223 + CA265_RS22325 0.12 -0.3 5,481,231 - CA265_RS22325 0.13 +0.1 5,481,233 + CA265_RS22325 0.13 +0.2 5,481,234 - CA265_RS22325 0.13 -0.6 5,481,235 + CA265_RS22325 0.14 +0.2 5,481,235 + CA265_RS22325 0.14 -0.3 5,481,281 + CA265_RS22325 0.17 -1.1 5,481,307 + CA265_RS22325 0.20 -0.2 5,481,329 + CA265_RS22325 0.22 +0.9 5,481,329 + CA265_RS22325 0.22 -0.2 5,481,330 - CA265_RS22325 0.22 +0.4
Or see this region's nucleotide sequence