Experiment: R2A with A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS21875 and CA265_RS21880 are separated by 113 nucleotides CA265_RS21880 and CA265_RS21885 are separated by 204 nucleotides CA265_RS21885 and CA265_RS21890 are separated by 12 nucleotides CA265_RS21890 and CA265_RS21895 are separated by 83 nucleotides
CA265_RS21875: CA265_RS21875 - 1,4-dihydroxy-6-naphthoate synthase, at 5,374,508 to 5,375,356
_RS21875
CA265_RS21880: CA265_RS21880 - futalosine hydrolase, at 5,375,470 to 5,376,093
_RS21880
CA265_RS21885: CA265_RS21885 - 6-pyruvoyl tetrahydrobiopterin synthase, at 5,376,298 to 5,376,708
_RS21885
CA265_RS21890: CA265_RS21890 - GTP cyclohydrolase I FolE, at 5,376,721 to 5,377,356
_RS21890
CA265_RS21895: CA265_RS21895 - [acyl-carrier-protein] S-malonyltransferase, at 5,377,440 to 5,378,321
_RS21895
Position (kb) 5376
5377 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5375.440 kb on + strand at 5375.444 kb on + strand at 5375.457 kb on + strand at 5375.467 kb on + strand at 5376.099 kb on - strand at 5376.135 kb on + strand at 5376.149 kb on + strand at 5376.150 kb on - strand at 5376.152 kb on + strand at 5376.153 kb on - strand at 5376.189 kb on + strand at 5376.219 kb on - strand at 5376.222 kb on - strand at 5376.222 kb on - strand at 5376.391 kb on - strand, within CA265_RS21885 at 5376.433 kb on + strand, within CA265_RS21885 at 5376.438 kb on + strand, within CA265_RS21885 at 5376.438 kb on + strand, within CA265_RS21885 at 5376.439 kb on - strand, within CA265_RS21885 at 5376.444 kb on + strand, within CA265_RS21885 at 5376.485 kb on + strand, within CA265_RS21885 at 5376.515 kb on - strand, within CA265_RS21885 at 5376.522 kb on - strand, within CA265_RS21885 at 5376.620 kb on + strand, within CA265_RS21885 at 5376.620 kb on + strand, within CA265_RS21885
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.02 mg/ml remove 5,375,440 + +0.5 5,375,444 + +0.2 5,375,457 + -0.5 5,375,467 + +0.8 5,376,099 - -1.1 5,376,135 + -0.9 5,376,149 + -0.5 5,376,150 - +0.3 5,376,152 + +0.9 5,376,153 - +0.5 5,376,189 + -0.5 5,376,219 - -0.8 5,376,222 - +0.0 5,376,222 - +0.1 5,376,391 - CA265_RS21885 0.23 -2.0 5,376,433 + CA265_RS21885 0.33 -1.1 5,376,438 + CA265_RS21885 0.34 +1.0 5,376,438 + CA265_RS21885 0.34 -1.0 5,376,439 - CA265_RS21885 0.34 -0.6 5,376,444 + CA265_RS21885 0.36 -1.0 5,376,485 + CA265_RS21885 0.45 -0.1 5,376,515 - CA265_RS21885 0.53 -1.0 5,376,522 - CA265_RS21885 0.55 -1.0 5,376,620 + CA265_RS21885 0.78 -1.3 5,376,620 + CA265_RS21885 0.78 -3.2
Or see this region's nucleotide sequence