Experiment: R2A with A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS21180 and CA265_RS21185 are separated by 450 nucleotides CA265_RS21185 and CA265_RS21190 are separated by 100 nucleotides CA265_RS21190 and CA265_RS21195 are separated by 97 nucleotides
CA265_RS21180: CA265_RS21180 - quinolinate synthase, at 5,214,308 to 5,215,306
_RS21180
CA265_RS21185: CA265_RS21185 - L-aspartate oxidase, at 5,215,757 to 5,217,343
_RS21185
CA265_RS21190: CA265_RS21190 - gamma carbonic anhydrase family protein, at 5,217,444 to 5,217,956
_RS21190
CA265_RS21195: CA265_RS21195 - hypothetical protein, at 5,218,054 to 5,219,697
_RS21195
Position (kb)
5215
5216
5217
5218 Strain fitness (log2 ratio)
-2
-1
0
1 at 5215.038 kb on - strand, within CA265_RS21180 at 5215.174 kb on - strand, within CA265_RS21180 at 5215.349 kb on - strand at 5215.369 kb on - strand at 5215.752 kb on + strand at 5215.940 kb on - strand, within CA265_RS21185 at 5216.047 kb on - strand, within CA265_RS21185 at 5216.190 kb on + strand, within CA265_RS21185 at 5216.191 kb on - strand, within CA265_RS21185 at 5216.273 kb on - strand, within CA265_RS21185 at 5216.282 kb on + strand, within CA265_RS21185 at 5216.292 kb on - strand, within CA265_RS21185 at 5216.376 kb on - strand, within CA265_RS21185 at 5216.489 kb on + strand, within CA265_RS21185 at 5216.553 kb on + strand, within CA265_RS21185 at 5216.588 kb on + strand, within CA265_RS21185 at 5216.599 kb on + strand at 5216.687 kb on - strand, within CA265_RS21185 at 5216.730 kb on + strand, within CA265_RS21185 at 5216.731 kb on - strand, within CA265_RS21185 at 5216.733 kb on - strand, within CA265_RS21185 at 5216.775 kb on - strand, within CA265_RS21185 at 5217.048 kb on + strand, within CA265_RS21185 at 5217.055 kb on - strand, within CA265_RS21185 at 5217.061 kb on + strand, within CA265_RS21185 at 5217.062 kb on - strand, within CA265_RS21185 at 5217.096 kb on - strand, within CA265_RS21185 at 5217.099 kb on + strand, within CA265_RS21185 at 5217.109 kb on - strand, within CA265_RS21185 at 5217.291 kb on - strand at 5217.347 kb on - strand at 5217.378 kb on + strand at 5217.383 kb on - strand at 5217.397 kb on - strand at 5217.437 kb on + strand at 5217.454 kb on + strand at 5217.474 kb on + strand at 5217.500 kb on + strand, within CA265_RS21190 at 5217.533 kb on - strand, within CA265_RS21190 at 5217.533 kb on - strand, within CA265_RS21190 at 5217.587 kb on - strand at 5217.596 kb on + strand, within CA265_RS21190 at 5217.662 kb on + strand, within CA265_RS21190 at 5217.662 kb on + strand, within CA265_RS21190 at 5217.662 kb on + strand, within CA265_RS21190 at 5217.695 kb on + strand, within CA265_RS21190 at 5217.703 kb on + strand, within CA265_RS21190 at 5217.703 kb on + strand, within CA265_RS21190 at 5217.707 kb on - strand, within CA265_RS21190 at 5217.715 kb on - strand, within CA265_RS21190 at 5217.717 kb on - strand, within CA265_RS21190 at 5217.783 kb on + strand, within CA265_RS21190 at 5217.788 kb on + strand, within CA265_RS21190 at 5217.789 kb on - strand, within CA265_RS21190 at 5217.841 kb on - strand, within CA265_RS21190 at 5217.844 kb on + strand, within CA265_RS21190 at 5217.845 kb on - strand, within CA265_RS21190 at 5217.901 kb on + strand, within CA265_RS21190 at 5217.924 kb on + strand at 5217.933 kb on + strand at 5217.933 kb on + strand at 5217.955 kb on - strand at 5218.008 kb on + strand at 5218.008 kb on + strand at 5218.121 kb on - strand at 5218.165 kb on + strand at 5218.194 kb on + strand at 5218.210 kb on + strand at 5218.221 kb on + strand, within CA265_RS21195 at 5218.223 kb on + strand, within CA265_RS21195 at 5218.224 kb on - strand, within CA265_RS21195
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.02 mg/ml remove 5,215,038 - CA265_RS21180 0.73 +1.1 5,215,174 - CA265_RS21180 0.87 -1.8 5,215,349 - +0.8 5,215,369 - -0.5 5,215,752 + -1.6 5,215,940 - CA265_RS21185 0.12 -1.0 5,216,047 - CA265_RS21185 0.18 +0.1 5,216,190 + CA265_RS21185 0.27 +1.5 5,216,191 - CA265_RS21185 0.27 +0.1 5,216,273 - CA265_RS21185 0.33 -0.9 5,216,282 + CA265_RS21185 0.33 -1.2 5,216,292 - CA265_RS21185 0.34 -1.0 5,216,376 - CA265_RS21185 0.39 -0.2 5,216,489 + CA265_RS21185 0.46 -0.9 5,216,553 + CA265_RS21185 0.50 -1.1 5,216,588 + CA265_RS21185 0.52 -0.6 5,216,599 + +1.1 5,216,687 - CA265_RS21185 0.59 -0.7 5,216,730 + CA265_RS21185 0.61 +0.5 5,216,731 - CA265_RS21185 0.61 -0.9 5,216,733 - CA265_RS21185 0.61 -0.5 5,216,775 - CA265_RS21185 0.64 -0.0 5,217,048 + CA265_RS21185 0.81 -1.2 5,217,055 - CA265_RS21185 0.82 -0.6 5,217,061 + CA265_RS21185 0.82 -1.6 5,217,062 - CA265_RS21185 0.82 -1.0 5,217,096 - CA265_RS21185 0.84 +0.8 5,217,099 + CA265_RS21185 0.85 -0.7 5,217,109 - CA265_RS21185 0.85 -0.5 5,217,291 - -2.3 5,217,347 - +0.1 5,217,378 + +0.8 5,217,383 - -0.0 5,217,397 - +0.3 5,217,437 + -0.4 5,217,454 + -1.6 5,217,474 + -0.7 5,217,500 + CA265_RS21190 0.11 -2.0 5,217,533 - CA265_RS21190 0.17 +0.8 5,217,533 - CA265_RS21190 0.17 -0.5 5,217,587 - +0.1 5,217,596 + CA265_RS21190 0.30 +1.2 5,217,662 + CA265_RS21190 0.42 -0.4 5,217,662 + CA265_RS21190 0.42 -0.9 5,217,662 + CA265_RS21190 0.42 -1.0 5,217,695 + CA265_RS21190 0.49 -1.1 5,217,703 + CA265_RS21190 0.50 +1.2 5,217,703 + CA265_RS21190 0.50 -0.9 5,217,707 - CA265_RS21190 0.51 -0.9 5,217,715 - CA265_RS21190 0.53 +0.1 5,217,717 - CA265_RS21190 0.53 +0.9 5,217,783 + CA265_RS21190 0.66 +1.1 5,217,788 + CA265_RS21190 0.67 -2.0 5,217,789 - CA265_RS21190 0.67 -0.4 5,217,841 - CA265_RS21190 0.77 -1.0 5,217,844 + CA265_RS21190 0.78 -0.5 5,217,845 - CA265_RS21190 0.78 +1.1 5,217,901 + CA265_RS21190 0.89 +0.3 5,217,924 + +0.4 5,217,933 + +1.3 5,217,933 + -0.3 5,217,955 - -0.2 5,218,008 + +0.6 5,218,008 + -0.5 5,218,121 - -0.6 5,218,165 + +0.5 5,218,194 + -0.5 5,218,210 + +0.7 5,218,221 + CA265_RS21195 0.10 -1.1 5,218,223 + CA265_RS21195 0.10 -0.3 5,218,224 - CA265_RS21195 0.10 -1.2
Or see this region's nucleotide sequence