Experiment: R2A with A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS20880 and CA265_RS20885 are separated by 53 nucleotides CA265_RS20885 and CA265_RS20890 are separated by 34 nucleotides CA265_RS20890 and CA265_RS20895 are separated by 131 nucleotides
CA265_RS20880: CA265_RS20880 - hypothetical protein, at 5,149,951 to 5,150,586
_RS20880
CA265_RS20885: CA265_RS20885 - iron-sulfur cluster biogenesis protein NfuA, at 5,150,640 to 5,150,885
_RS20885
CA265_RS20890: CA265_RS20890 - MRP family ATP-binding protein, at 5,150,920 to 5,151,996
_RS20890
CA265_RS20895: CA265_RS20895 - penicillin-binding protein, at 5,152,128 to 5,154,287
_RS20895
Position (kb)
5150
5151
5152 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5149.994 kb on - strand at 5150.016 kb on + strand, within CA265_RS20880 at 5150.016 kb on + strand, within CA265_RS20880 at 5150.041 kb on - strand, within CA265_RS20880 at 5150.041 kb on - strand, within CA265_RS20880 at 5150.046 kb on + strand, within CA265_RS20880 at 5150.069 kb on + strand, within CA265_RS20880 at 5150.076 kb on - strand, within CA265_RS20880 at 5150.111 kb on - strand, within CA265_RS20880 at 5150.170 kb on - strand, within CA265_RS20880 at 5150.377 kb on + strand, within CA265_RS20880 at 5150.389 kb on - strand, within CA265_RS20880 at 5150.453 kb on + strand, within CA265_RS20880 at 5150.461 kb on - strand, within CA265_RS20880 at 5150.579 kb on + strand at 5150.580 kb on - strand at 5150.605 kb on - strand at 5150.607 kb on + strand at 5150.625 kb on - strand at 5150.866 kb on - strand at 5150.891 kb on - strand at 5150.893 kb on - strand at 5151.315 kb on + strand, within CA265_RS20890 at 5151.590 kb on + strand, within CA265_RS20890 at 5151.772 kb on + strand, within CA265_RS20890 at 5151.790 kb on + strand, within CA265_RS20890 at 5152.000 kb on - strand at 5152.002 kb on + strand at 5152.045 kb on + strand at 5152.046 kb on - strand at 5152.088 kb on + strand at 5152.110 kb on - strand at 5152.198 kb on + strand at 5152.216 kb on + strand at 5152.232 kb on + strand at 5152.281 kb on - strand at 5152.361 kb on + strand, within CA265_RS20895 at 5152.362 kb on - strand, within CA265_RS20895 at 5152.389 kb on - strand, within CA265_RS20895 at 5152.401 kb on - strand, within CA265_RS20895 at 5152.429 kb on + strand, within CA265_RS20895 at 5152.495 kb on + strand, within CA265_RS20895 at 5152.496 kb on - strand, within CA265_RS20895 at 5152.556 kb on + strand, within CA265_RS20895 at 5152.592 kb on - strand, within CA265_RS20895 at 5152.637 kb on + strand, within CA265_RS20895 at 5152.679 kb on + strand, within CA265_RS20895 at 5152.680 kb on - strand, within CA265_RS20895 at 5152.783 kb on - strand, within CA265_RS20895 at 5152.786 kb on + strand, within CA265_RS20895 at 5152.807 kb on + strand, within CA265_RS20895 at 5152.889 kb on + strand, within CA265_RS20895 at 5152.936 kb on - strand, within CA265_RS20895
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.02 mg/ml remove 5,149,994 - +0.7 5,150,016 + CA265_RS20880 0.10 -0.8 5,150,016 + CA265_RS20880 0.10 +0.0 5,150,041 - CA265_RS20880 0.14 +0.2 5,150,041 - CA265_RS20880 0.14 -0.7 5,150,046 + CA265_RS20880 0.15 +0.3 5,150,069 + CA265_RS20880 0.19 +0.9 5,150,076 - CA265_RS20880 0.20 +0.3 5,150,111 - CA265_RS20880 0.25 +0.7 5,150,170 - CA265_RS20880 0.34 +0.3 5,150,377 + CA265_RS20880 0.67 +0.2 5,150,389 - CA265_RS20880 0.69 +1.0 5,150,453 + CA265_RS20880 0.79 -1.2 5,150,461 - CA265_RS20880 0.80 -0.1 5,150,579 + -2.1 5,150,580 - -1.6 5,150,605 - +0.0 5,150,607 + -0.7 5,150,625 - -0.5 5,150,866 - +0.1 5,150,891 - -0.9 5,150,893 - +0.1 5,151,315 + CA265_RS20890 0.37 -2.0 5,151,590 + CA265_RS20890 0.62 -0.5 5,151,772 + CA265_RS20890 0.79 -0.2 5,151,790 + CA265_RS20890 0.81 +0.1 5,152,000 - -1.8 5,152,002 + +1.2 5,152,045 + +0.3 5,152,046 - -1.5 5,152,088 + +0.6 5,152,110 - +1.5 5,152,198 + -0.1 5,152,216 + -1.6 5,152,232 + -0.2 5,152,281 - +0.6 5,152,361 + CA265_RS20895 0.11 -1.2 5,152,362 - CA265_RS20895 0.11 +2.1 5,152,389 - CA265_RS20895 0.12 -0.4 5,152,401 - CA265_RS20895 0.13 -0.0 5,152,429 + CA265_RS20895 0.14 -0.5 5,152,495 + CA265_RS20895 0.17 -0.3 5,152,496 - CA265_RS20895 0.17 -0.2 5,152,556 + CA265_RS20895 0.20 -1.3 5,152,592 - CA265_RS20895 0.21 +1.8 5,152,637 + CA265_RS20895 0.24 -0.4 5,152,679 + CA265_RS20895 0.26 -0.7 5,152,680 - CA265_RS20895 0.26 -0.9 5,152,783 - CA265_RS20895 0.30 +1.1 5,152,786 + CA265_RS20895 0.30 -0.0 5,152,807 + CA265_RS20895 0.31 -0.4 5,152,889 + CA265_RS20895 0.35 +1.6 5,152,936 - CA265_RS20895 0.37 -0.9
Or see this region's nucleotide sequence