Experiment: R2A with A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS20520 and CA265_RS20525 are separated by 4 nucleotides CA265_RS20525 and CA265_RS20530 are separated by 27 nucleotides CA265_RS20530 and CA265_RS20535 are separated by 215 nucleotides CA265_RS20535 and CA265_RS20540 are separated by 102 nucleotides CA265_RS20540 and CA265_RS20545 are separated by 22 nucleotides
CA265_RS20520: CA265_RS20520 - hypothetical protein, at 5,080,481 to 5,081,302
_RS20520
CA265_RS20525: CA265_RS20525 - hypothetical protein, at 5,081,307 to 5,081,555
_RS20525
CA265_RS20530: CA265_RS20530 - hypothetical protein, at 5,081,583 to 5,081,780
_RS20530
CA265_RS20535: CA265_RS20535 - hypothetical protein, at 5,081,996 to 5,082,430
_RS20535
CA265_RS20540: CA265_RS20540 - hypothetical protein, at 5,082,533 to 5,083,168
_RS20540
CA265_RS20545: CA265_RS20545 - 2-amino-3-ketobutyrate CoA ligase, at 5,083,191 to 5,084,453
_RS20545
Position (kb) 5081
5082
5083 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5081.023 kb on + strand, within CA265_RS20520 at 5081.159 kb on - strand, within CA265_RS20520 at 5081.163 kb on - strand, within CA265_RS20520 at 5081.185 kb on - strand, within CA265_RS20520 at 5081.197 kb on + strand, within CA265_RS20520 at 5081.197 kb on + strand, within CA265_RS20520 at 5081.252 kb on - strand at 5081.252 kb on - strand at 5081.260 kb on + strand at 5081.292 kb on + strand at 5081.292 kb on + strand at 5081.292 kb on + strand at 5081.292 kb on + strand at 5081.295 kb on + strand at 5081.302 kb on + strand at 5081.303 kb on - strand at 5081.521 kb on + strand, within CA265_RS20525 at 5081.521 kb on + strand, within CA265_RS20525 at 5081.522 kb on - strand, within CA265_RS20525 at 5081.566 kb on + strand at 5081.744 kb on + strand, within CA265_RS20530 at 5081.770 kb on + strand at 5081.770 kb on + strand at 5081.800 kb on + strand at 5081.814 kb on + strand at 5081.858 kb on + strand at 5081.864 kb on + strand at 5081.891 kb on + strand at 5081.891 kb on + strand at 5081.893 kb on + strand at 5081.894 kb on - strand at 5081.902 kb on + strand at 5081.902 kb on + strand at 5081.902 kb on + strand at 5081.903 kb on - strand at 5081.903 kb on - strand at 5081.907 kb on + strand at 5081.915 kb on + strand at 5081.966 kb on - strand at 5081.966 kb on - strand at 5082.031 kb on - strand at 5082.111 kb on - strand, within CA265_RS20535 at 5082.212 kb on + strand, within CA265_RS20535 at 5082.213 kb on - strand, within CA265_RS20535 at 5082.313 kb on + strand, within CA265_RS20535 at 5082.314 kb on - strand, within CA265_RS20535 at 5082.314 kb on - strand, within CA265_RS20535 at 5082.319 kb on - strand, within CA265_RS20535 at 5082.354 kb on + strand, within CA265_RS20535 at 5082.626 kb on + strand, within CA265_RS20540 at 5082.656 kb on + strand, within CA265_RS20540 at 5082.657 kb on - strand, within CA265_RS20540 at 5082.661 kb on + strand, within CA265_RS20540 at 5082.661 kb on + strand, within CA265_RS20540 at 5082.662 kb on - strand, within CA265_RS20540 at 5082.666 kb on + strand, within CA265_RS20540 at 5082.666 kb on + strand, within CA265_RS20540 at 5082.710 kb on - strand, within CA265_RS20540 at 5082.730 kb on + strand, within CA265_RS20540 at 5082.844 kb on + strand, within CA265_RS20540 at 5082.971 kb on - strand, within CA265_RS20540 at 5082.972 kb on + strand, within CA265_RS20540 at 5083.011 kb on + strand, within CA265_RS20540 at 5083.012 kb on - strand, within CA265_RS20540 at 5083.086 kb on - strand, within CA265_RS20540 at 5083.150 kb on + strand at 5083.185 kb on + strand at 5083.188 kb on - strand at 5083.327 kb on + strand, within CA265_RS20545 at 5083.328 kb on - strand, within CA265_RS20545 at 5083.345 kb on + strand, within CA265_RS20545 at 5083.374 kb on + strand, within CA265_RS20545
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.02 mg/ml remove 5,081,023 + CA265_RS20520 0.66 +1.4 5,081,159 - CA265_RS20520 0.82 +0.3 5,081,163 - CA265_RS20520 0.83 -1.2 5,081,185 - CA265_RS20520 0.86 +0.8 5,081,197 + CA265_RS20520 0.87 -1.7 5,081,197 + CA265_RS20520 0.87 -2.2 5,081,252 - +0.8 5,081,252 - -0.4 5,081,260 + -1.0 5,081,292 + -0.2 5,081,292 + +0.6 5,081,292 + -0.0 5,081,292 + +0.2 5,081,295 + -0.0 5,081,302 + +2.1 5,081,303 - -0.1 5,081,521 + CA265_RS20525 0.86 +1.1 5,081,521 + CA265_RS20525 0.86 -1.1 5,081,522 - CA265_RS20525 0.86 +0.9 5,081,566 + +0.4 5,081,744 + CA265_RS20530 0.81 -0.3 5,081,770 + -0.6 5,081,770 + +0.9 5,081,800 + +0.1 5,081,814 + -0.1 5,081,858 + +0.2 5,081,864 + -1.1 5,081,891 + -0.4 5,081,891 + +0.7 5,081,893 + -0.4 5,081,894 - -0.5 5,081,902 + -1.2 5,081,902 + -0.6 5,081,902 + +1.0 5,081,903 - +0.4 5,081,903 - -0.2 5,081,907 + -0.9 5,081,915 + -1.0 5,081,966 - -0.8 5,081,966 - -0.4 5,082,031 - -0.6 5,082,111 - CA265_RS20535 0.26 -0.3 5,082,212 + CA265_RS20535 0.50 -1.6 5,082,213 - CA265_RS20535 0.50 -0.8 5,082,313 + CA265_RS20535 0.73 -0.2 5,082,314 - CA265_RS20535 0.73 -0.8 5,082,314 - CA265_RS20535 0.73 -1.2 5,082,319 - CA265_RS20535 0.74 +1.1 5,082,354 + CA265_RS20535 0.82 -0.6 5,082,626 + CA265_RS20540 0.15 -0.6 5,082,656 + CA265_RS20540 0.19 +0.5 5,082,657 - CA265_RS20540 0.19 -0.4 5,082,661 + CA265_RS20540 0.20 -0.3 5,082,661 + CA265_RS20540 0.20 +0.6 5,082,662 - CA265_RS20540 0.20 +0.1 5,082,666 + CA265_RS20540 0.21 +0.5 5,082,666 + CA265_RS20540 0.21 +0.1 5,082,710 - CA265_RS20540 0.28 -1.5 5,082,730 + CA265_RS20540 0.31 +0.4 5,082,844 + CA265_RS20540 0.49 +0.5 5,082,971 - CA265_RS20540 0.69 -0.2 5,082,972 + CA265_RS20540 0.69 -1.7 5,083,011 + CA265_RS20540 0.75 +0.7 5,083,012 - CA265_RS20540 0.75 -0.8 5,083,086 - CA265_RS20540 0.87 -1.1 5,083,150 + -0.1 5,083,185 + -0.3 5,083,188 - +1.1 5,083,327 + CA265_RS20545 0.11 -0.4 5,083,328 - CA265_RS20545 0.11 -0.5 5,083,345 + CA265_RS20545 0.12 -1.3 5,083,374 + CA265_RS20545 0.14 +0.3
Or see this region's nucleotide sequence