Experiment: R2A with A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS20520 and CA265_RS20525 are separated by 4 nucleotides CA265_RS20525 and CA265_RS20530 are separated by 27 nucleotides CA265_RS20530 and CA265_RS20535 are separated by 215 nucleotides CA265_RS20535 and CA265_RS20540 are separated by 102 nucleotides
CA265_RS20520: CA265_RS20520 - hypothetical protein, at 5,080,481 to 5,081,302
_RS20520
CA265_RS20525: CA265_RS20525 - hypothetical protein, at 5,081,307 to 5,081,555
_RS20525
CA265_RS20530: CA265_RS20530 - hypothetical protein, at 5,081,583 to 5,081,780
_RS20530
CA265_RS20535: CA265_RS20535 - hypothetical protein, at 5,081,996 to 5,082,430
_RS20535
CA265_RS20540: CA265_RS20540 - hypothetical protein, at 5,082,533 to 5,083,168
_RS20540
Position (kb)
5081
5082 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5080.348 kb on - strand at 5080.356 kb on + strand at 5080.356 kb on + strand at 5080.356 kb on + strand at 5080.357 kb on - strand at 5080.376 kb on + strand at 5080.377 kb on - strand at 5080.377 kb on - strand at 5080.382 kb on - strand at 5080.387 kb on - strand at 5080.395 kb on + strand at 5080.395 kb on + strand at 5080.472 kb on - strand at 5080.475 kb on - strand at 5080.483 kb on - strand at 5080.528 kb on + strand at 5080.528 kb on + strand at 5080.705 kb on + strand, within CA265_RS20520 at 5080.706 kb on - strand, within CA265_RS20520 at 5080.706 kb on - strand, within CA265_RS20520 at 5080.706 kb on - strand, within CA265_RS20520 at 5080.706 kb on - strand, within CA265_RS20520 at 5080.715 kb on - strand, within CA265_RS20520 at 5080.786 kb on + strand, within CA265_RS20520 at 5080.826 kb on - strand, within CA265_RS20520 at 5080.827 kb on + strand, within CA265_RS20520 at 5080.828 kb on - strand, within CA265_RS20520 at 5080.828 kb on - strand, within CA265_RS20520 at 5080.829 kb on + strand, within CA265_RS20520 at 5080.829 kb on + strand, within CA265_RS20520 at 5080.849 kb on - strand, within CA265_RS20520 at 5080.925 kb on - strand, within CA265_RS20520 at 5081.023 kb on + strand, within CA265_RS20520 at 5081.159 kb on - strand, within CA265_RS20520 at 5081.163 kb on - strand, within CA265_RS20520 at 5081.185 kb on - strand, within CA265_RS20520 at 5081.197 kb on + strand, within CA265_RS20520 at 5081.197 kb on + strand, within CA265_RS20520 at 5081.252 kb on - strand at 5081.252 kb on - strand at 5081.260 kb on + strand at 5081.292 kb on + strand at 5081.292 kb on + strand at 5081.292 kb on + strand at 5081.292 kb on + strand at 5081.295 kb on + strand at 5081.302 kb on + strand at 5081.303 kb on - strand at 5081.521 kb on + strand, within CA265_RS20525 at 5081.521 kb on + strand, within CA265_RS20525 at 5081.522 kb on - strand, within CA265_RS20525 at 5081.566 kb on + strand at 5081.744 kb on + strand, within CA265_RS20530 at 5081.770 kb on + strand at 5081.770 kb on + strand at 5081.800 kb on + strand at 5081.814 kb on + strand at 5081.858 kb on + strand at 5081.864 kb on + strand at 5081.891 kb on + strand at 5081.891 kb on + strand at 5081.893 kb on + strand at 5081.894 kb on - strand at 5081.902 kb on + strand at 5081.902 kb on + strand at 5081.902 kb on + strand at 5081.903 kb on - strand at 5081.903 kb on - strand at 5081.907 kb on + strand at 5081.915 kb on + strand at 5081.966 kb on - strand at 5081.966 kb on - strand at 5082.031 kb on - strand at 5082.111 kb on - strand, within CA265_RS20535 at 5082.212 kb on + strand, within CA265_RS20535 at 5082.213 kb on - strand, within CA265_RS20535 at 5082.313 kb on + strand, within CA265_RS20535 at 5082.314 kb on - strand, within CA265_RS20535 at 5082.314 kb on - strand, within CA265_RS20535 at 5082.319 kb on - strand, within CA265_RS20535 at 5082.354 kb on + strand, within CA265_RS20535
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.02 mg/ml remove 5,080,348 - -0.5 5,080,356 + +0.3 5,080,356 + -0.1 5,080,356 + +0.9 5,080,357 - +0.5 5,080,376 + +0.5 5,080,377 - -0.7 5,080,377 - +0.8 5,080,382 - -1.2 5,080,387 - -0.7 5,080,395 + -1.3 5,080,395 + -0.3 5,080,472 - -0.7 5,080,475 - -0.5 5,080,483 - -0.7 5,080,528 + +0.8 5,080,528 + +0.6 5,080,705 + CA265_RS20520 0.27 -0.5 5,080,706 - CA265_RS20520 0.27 -0.5 5,080,706 - CA265_RS20520 0.27 +0.0 5,080,706 - CA265_RS20520 0.27 +0.1 5,080,706 - CA265_RS20520 0.27 -0.2 5,080,715 - CA265_RS20520 0.28 +1.4 5,080,786 + CA265_RS20520 0.37 -0.1 5,080,826 - CA265_RS20520 0.42 -0.5 5,080,827 + CA265_RS20520 0.42 -0.1 5,080,828 - CA265_RS20520 0.42 -0.1 5,080,828 - CA265_RS20520 0.42 -0.7 5,080,829 + CA265_RS20520 0.42 +0.3 5,080,829 + CA265_RS20520 0.42 -1.1 5,080,849 - CA265_RS20520 0.45 -0.1 5,080,925 - CA265_RS20520 0.54 -0.4 5,081,023 + CA265_RS20520 0.66 +1.4 5,081,159 - CA265_RS20520 0.82 +0.3 5,081,163 - CA265_RS20520 0.83 -1.2 5,081,185 - CA265_RS20520 0.86 +0.8 5,081,197 + CA265_RS20520 0.87 -1.7 5,081,197 + CA265_RS20520 0.87 -2.2 5,081,252 - +0.8 5,081,252 - -0.4 5,081,260 + -1.0 5,081,292 + -0.2 5,081,292 + +0.6 5,081,292 + -0.0 5,081,292 + +0.2 5,081,295 + -0.0 5,081,302 + +2.1 5,081,303 - -0.1 5,081,521 + CA265_RS20525 0.86 +1.1 5,081,521 + CA265_RS20525 0.86 -1.1 5,081,522 - CA265_RS20525 0.86 +0.9 5,081,566 + +0.4 5,081,744 + CA265_RS20530 0.81 -0.3 5,081,770 + -0.6 5,081,770 + +0.9 5,081,800 + +0.1 5,081,814 + -0.1 5,081,858 + +0.2 5,081,864 + -1.1 5,081,891 + -0.4 5,081,891 + +0.7 5,081,893 + -0.4 5,081,894 - -0.5 5,081,902 + -1.2 5,081,902 + -0.6 5,081,902 + +1.0 5,081,903 - +0.4 5,081,903 - -0.2 5,081,907 + -0.9 5,081,915 + -1.0 5,081,966 - -0.8 5,081,966 - -0.4 5,082,031 - -0.6 5,082,111 - CA265_RS20535 0.26 -0.3 5,082,212 + CA265_RS20535 0.50 -1.6 5,082,213 - CA265_RS20535 0.50 -0.8 5,082,313 + CA265_RS20535 0.73 -0.2 5,082,314 - CA265_RS20535 0.73 -0.8 5,082,314 - CA265_RS20535 0.73 -1.2 5,082,319 - CA265_RS20535 0.74 +1.1 5,082,354 + CA265_RS20535 0.82 -0.6
Or see this region's nucleotide sequence