Experiment: R2A with A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS20460 and CA265_RS20465 are separated by 263 nucleotides CA265_RS20465 and CA265_RS20470 are separated by 33 nucleotides
CA265_RS20460: CA265_RS20460 - hypothetical protein, at 5,060,130 to 5,061,224
_RS20460
CA265_RS20465: CA265_RS20465 - hypothetical protein, at 5,061,488 to 5,061,646
_RS20465
CA265_RS20470: CA265_RS20470 - DNA ligase D, at 5,061,680 to 5,064,571
_RS20470
Position (kb)
5061
5062 Strain fitness (log2 ratio)
-2
-1
0
1 at 5060.499 kb on - strand at 5060.501 kb on + strand, within CA265_RS20460 at 5060.507 kb on - strand, within CA265_RS20460 at 5060.621 kb on + strand, within CA265_RS20460 at 5060.624 kb on + strand, within CA265_RS20460 at 5060.624 kb on + strand, within CA265_RS20460 at 5060.625 kb on - strand, within CA265_RS20460 at 5060.625 kb on - strand, within CA265_RS20460 at 5060.626 kb on + strand, within CA265_RS20460 at 5060.709 kb on + strand, within CA265_RS20460 at 5060.710 kb on - strand, within CA265_RS20460 at 5060.710 kb on - strand, within CA265_RS20460 at 5060.739 kb on + strand, within CA265_RS20460 at 5060.740 kb on - strand, within CA265_RS20460 at 5060.746 kb on + strand, within CA265_RS20460 at 5060.750 kb on + strand, within CA265_RS20460 at 5060.750 kb on + strand, within CA265_RS20460 at 5060.805 kb on - strand, within CA265_RS20460 at 5060.805 kb on - strand, within CA265_RS20460 at 5060.873 kb on - strand, within CA265_RS20460 at 5060.886 kb on + strand, within CA265_RS20460 at 5060.974 kb on + strand, within CA265_RS20460 at 5060.979 kb on - strand, within CA265_RS20460 at 5061.045 kb on + strand, within CA265_RS20460 at 5061.046 kb on - strand, within CA265_RS20460 at 5061.046 kb on - strand, within CA265_RS20460 at 5061.070 kb on + strand, within CA265_RS20460 at 5061.075 kb on - strand, within CA265_RS20460 at 5061.099 kb on + strand, within CA265_RS20460 at 5061.099 kb on + strand, within CA265_RS20460 at 5061.100 kb on - strand, within CA265_RS20460 at 5061.160 kb on - strand at 5061.229 kb on - strand at 5061.238 kb on - strand at 5061.302 kb on + strand at 5061.406 kb on - strand at 5061.406 kb on - strand at 5061.426 kb on + strand at 5061.426 kb on + strand at 5061.482 kb on - strand at 5061.556 kb on + strand, within CA265_RS20465 at 5061.556 kb on + strand, within CA265_RS20465 at 5061.559 kb on + strand, within CA265_RS20465 at 5061.696 kb on - strand at 5061.696 kb on - strand at 5061.700 kb on + strand at 5061.701 kb on - strand at 5061.711 kb on + strand at 5061.723 kb on + strand at 5061.768 kb on + strand at 5061.769 kb on - strand at 5061.769 kb on - strand at 5061.819 kb on + strand at 5061.879 kb on + strand at 5061.879 kb on + strand at 5061.880 kb on - strand at 5061.940 kb on + strand at 5061.940 kb on + strand at 5061.953 kb on + strand at 5061.953 kb on + strand at 5061.972 kb on - strand, within CA265_RS20470 at 5062.151 kb on + strand, within CA265_RS20470 at 5062.151 kb on + strand, within CA265_RS20470 at 5062.152 kb on - strand, within CA265_RS20470 at 5062.152 kb on - strand, within CA265_RS20470 at 5062.153 kb on + strand, within CA265_RS20470 at 5062.154 kb on - strand, within CA265_RS20470 at 5062.274 kb on + strand, within CA265_RS20470 at 5062.274 kb on + strand, within CA265_RS20470 at 5062.290 kb on - strand, within CA265_RS20470 at 5062.304 kb on + strand, within CA265_RS20470 at 5062.304 kb on + strand, within CA265_RS20470 at 5062.324 kb on + strand, within CA265_RS20470 at 5062.330 kb on - strand, within CA265_RS20470 at 5062.342 kb on - strand, within CA265_RS20470 at 5062.603 kb on - strand, within CA265_RS20470 at 5062.636 kb on - strand, within CA265_RS20470 at 5062.638 kb on - strand, within CA265_RS20470
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.02 mg/ml remove 5,060,499 - +0.1 5,060,501 + CA265_RS20460 0.34 +0.7 5,060,507 - CA265_RS20460 0.34 -0.2 5,060,621 + CA265_RS20460 0.45 -1.0 5,060,624 + CA265_RS20460 0.45 +0.2 5,060,624 + CA265_RS20460 0.45 -1.9 5,060,625 - CA265_RS20460 0.45 -0.8 5,060,625 - CA265_RS20460 0.45 -0.2 5,060,626 + CA265_RS20460 0.45 +0.2 5,060,709 + CA265_RS20460 0.53 +0.7 5,060,710 - CA265_RS20460 0.53 -0.9 5,060,710 - CA265_RS20460 0.53 +0.6 5,060,739 + CA265_RS20460 0.56 +1.3 5,060,740 - CA265_RS20460 0.56 -0.9 5,060,746 + CA265_RS20460 0.56 +0.6 5,060,750 + CA265_RS20460 0.57 -0.7 5,060,750 + CA265_RS20460 0.57 +0.1 5,060,805 - CA265_RS20460 0.62 +0.2 5,060,805 - CA265_RS20460 0.62 -0.6 5,060,873 - CA265_RS20460 0.68 -1.5 5,060,886 + CA265_RS20460 0.69 -1.7 5,060,974 + CA265_RS20460 0.77 -0.8 5,060,979 - CA265_RS20460 0.78 +0.1 5,061,045 + CA265_RS20460 0.84 -0.5 5,061,046 - CA265_RS20460 0.84 -0.7 5,061,046 - CA265_RS20460 0.84 -0.2 5,061,070 + CA265_RS20460 0.86 -0.7 5,061,075 - CA265_RS20460 0.86 -0.1 5,061,099 + CA265_RS20460 0.88 -0.5 5,061,099 + CA265_RS20460 0.88 -1.3 5,061,100 - CA265_RS20460 0.89 +1.4 5,061,160 - +0.1 5,061,229 - -2.0 5,061,238 - -0.5 5,061,302 + -0.9 5,061,406 - +0.1 5,061,406 - +0.2 5,061,426 + +0.6 5,061,426 + -1.6 5,061,482 - -0.2 5,061,556 + CA265_RS20465 0.43 -1.5 5,061,556 + CA265_RS20465 0.43 -0.6 5,061,559 + CA265_RS20465 0.45 +0.5 5,061,696 - +0.2 5,061,696 - -0.4 5,061,700 + -0.5 5,061,701 - +0.2 5,061,711 + +0.2 5,061,723 + -2.0 5,061,768 + +0.8 5,061,769 - +0.1 5,061,769 - +0.5 5,061,819 + -1.1 5,061,879 + +1.3 5,061,879 + -0.7 5,061,880 - +0.0 5,061,940 + +0.3 5,061,940 + +0.3 5,061,953 + -0.4 5,061,953 + -0.7 5,061,972 - CA265_RS20470 0.10 -1.8 5,062,151 + CA265_RS20470 0.16 +0.1 5,062,151 + CA265_RS20470 0.16 +0.6 5,062,152 - CA265_RS20470 0.16 -0.9 5,062,152 - CA265_RS20470 0.16 -0.1 5,062,153 + CA265_RS20470 0.16 -0.1 5,062,154 - CA265_RS20470 0.16 -0.5 5,062,274 + CA265_RS20470 0.21 +0.5 5,062,274 + CA265_RS20470 0.21 +0.5 5,062,290 - CA265_RS20470 0.21 +0.2 5,062,304 + CA265_RS20470 0.22 -0.8 5,062,304 + CA265_RS20470 0.22 -1.0 5,062,324 + CA265_RS20470 0.22 +0.1 5,062,330 - CA265_RS20470 0.22 +0.1 5,062,342 - CA265_RS20470 0.23 -0.6 5,062,603 - CA265_RS20470 0.32 -0.5 5,062,636 - CA265_RS20470 0.33 -0.1 5,062,638 - CA265_RS20470 0.33 +0.5
Or see this region's nucleotide sequence