Experiment: R2A with A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS19235 and CA265_RS19240 are separated by 4 nucleotides CA265_RS19240 and CA265_RS19245 are separated by 134 nucleotides CA265_RS19245 and CA265_RS19250 are separated by 20 nucleotides
CA265_RS19235: CA265_RS19235 - hypothetical protein, at 4,779,965 to 4,780,840
_RS19235
CA265_RS19240: CA265_RS19240 - hypothetical protein, at 4,780,845 to 4,781,102
_RS19240
CA265_RS19245: CA265_RS19245 - hypothetical protein, at 4,781,237 to 4,781,827
_RS19245
CA265_RS19250: CA265_RS19250 - hypothetical protein, at 4,781,848 to 4,783,062
_RS19250
Position (kb)
4781
4782 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 4780.335 kb on - strand, within CA265_RS19235 at 4780.345 kb on + strand, within CA265_RS19235 at 4780.798 kb on + strand at 4780.999 kb on + strand, within CA265_RS19240 at 4780.999 kb on + strand, within CA265_RS19240 at 4781.109 kb on + strand at 4781.125 kb on + strand at 4781.164 kb on + strand at 4781.186 kb on - strand at 4781.196 kb on + strand at 4781.223 kb on + strand at 4781.242 kb on + strand at 4781.242 kb on + strand at 4781.274 kb on + strand at 4781.274 kb on + strand at 4781.274 kb on + strand at 4781.274 kb on + strand at 4781.275 kb on - strand at 4781.276 kb on + strand at 4781.276 kb on + strand at 4781.276 kb on + strand at 4781.277 kb on - strand at 4781.277 kb on - strand at 4781.311 kb on + strand, within CA265_RS19245 at 4781.324 kb on + strand, within CA265_RS19245 at 4781.325 kb on - strand, within CA265_RS19245 at 4781.325 kb on - strand, within CA265_RS19245 at 4781.393 kb on + strand, within CA265_RS19245 at 4781.426 kb on - strand, within CA265_RS19245 at 4781.495 kb on + strand, within CA265_RS19245 at 4781.496 kb on - strand, within CA265_RS19245 at 4781.496 kb on - strand, within CA265_RS19245 at 4781.533 kb on + strand, within CA265_RS19245 at 4781.533 kb on + strand, within CA265_RS19245 at 4781.534 kb on - strand, within CA265_RS19245 at 4781.537 kb on + strand, within CA265_RS19245 at 4781.538 kb on - strand, within CA265_RS19245 at 4781.538 kb on - strand, within CA265_RS19245 at 4781.550 kb on - strand, within CA265_RS19245 at 4781.607 kb on - strand, within CA265_RS19245 at 4781.619 kb on - strand, within CA265_RS19245 at 4781.628 kb on - strand, within CA265_RS19245 at 4781.638 kb on + strand, within CA265_RS19245 at 4781.639 kb on - strand, within CA265_RS19245 at 4781.643 kb on + strand, within CA265_RS19245 at 4781.649 kb on + strand, within CA265_RS19245 at 4781.649 kb on + strand, within CA265_RS19245 at 4781.649 kb on + strand, within CA265_RS19245 at 4781.650 kb on - strand, within CA265_RS19245 at 4781.650 kb on - strand, within CA265_RS19245 at 4781.693 kb on + strand, within CA265_RS19245 at 4781.693 kb on + strand, within CA265_RS19245 at 4781.694 kb on - strand, within CA265_RS19245 at 4781.696 kb on - strand, within CA265_RS19245 at 4781.721 kb on + strand, within CA265_RS19245 at 4781.725 kb on + strand, within CA265_RS19245 at 4781.819 kb on - strand at 4781.827 kb on + strand at 4781.828 kb on - strand at 4781.836 kb on - strand at 4781.912 kb on + strand at 4782.019 kb on + strand, within CA265_RS19250 at 4782.071 kb on - strand, within CA265_RS19250 at 4782.071 kb on - strand, within CA265_RS19250 at 4782.071 kb on - strand, within CA265_RS19250 at 4782.071 kb on - strand, within CA265_RS19250 at 4782.163 kb on - strand, within CA265_RS19250 at 4782.176 kb on + strand, within CA265_RS19250 at 4782.191 kb on + strand, within CA265_RS19250 at 4782.195 kb on - strand, within CA265_RS19250 at 4782.289 kb on + strand, within CA265_RS19250 at 4782.289 kb on + strand, within CA265_RS19250 at 4782.291 kb on + strand, within CA265_RS19250 at 4782.291 kb on + strand, within CA265_RS19250 at 4782.292 kb on - strand, within CA265_RS19250 at 4782.338 kb on + strand, within CA265_RS19250 at 4782.338 kb on + strand, within CA265_RS19250 at 4782.383 kb on + strand, within CA265_RS19250 at 4782.383 kb on + strand, within CA265_RS19250 at 4782.428 kb on + strand, within CA265_RS19250 at 4782.429 kb on - strand, within CA265_RS19250 at 4782.463 kb on - strand, within CA265_RS19250 at 4782.602 kb on - strand, within CA265_RS19250 at 4782.663 kb on - strand at 4782.668 kb on - strand, within CA265_RS19250 at 4782.670 kb on - strand, within CA265_RS19250 at 4782.777 kb on + strand, within CA265_RS19250 at 4782.777 kb on + strand, within CA265_RS19250
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.02 mg/ml remove 4,780,335 - CA265_RS19235 0.42 -0.0 4,780,345 + CA265_RS19235 0.43 -2.9 4,780,798 + -0.8 4,780,999 + CA265_RS19240 0.60 -3.4 4,780,999 + CA265_RS19240 0.60 -2.3 4,781,109 + -4.3 4,781,125 + -2.3 4,781,164 + +0.4 4,781,186 - -0.5 4,781,196 + -0.9 4,781,223 + -1.6 4,781,242 + -0.4 4,781,242 + -0.9 4,781,274 + -2.0 4,781,274 + -0.6 4,781,274 + -0.9 4,781,274 + -1.5 4,781,275 - -0.0 4,781,276 + -0.4 4,781,276 + -0.7 4,781,276 + -0.9 4,781,277 - +0.3 4,781,277 - -0.2 4,781,311 + CA265_RS19245 0.13 -2.1 4,781,324 + CA265_RS19245 0.15 -0.7 4,781,325 - CA265_RS19245 0.15 -1.1 4,781,325 - CA265_RS19245 0.15 -1.8 4,781,393 + CA265_RS19245 0.26 -1.4 4,781,426 - CA265_RS19245 0.32 -0.7 4,781,495 + CA265_RS19245 0.44 -0.7 4,781,496 - CA265_RS19245 0.44 -0.6 4,781,496 - CA265_RS19245 0.44 -1.0 4,781,533 + CA265_RS19245 0.50 -0.2 4,781,533 + CA265_RS19245 0.50 +3.0 4,781,534 - CA265_RS19245 0.50 -0.1 4,781,537 + CA265_RS19245 0.51 -1.5 4,781,538 - CA265_RS19245 0.51 -1.2 4,781,538 - CA265_RS19245 0.51 +0.4 4,781,550 - CA265_RS19245 0.53 -0.5 4,781,607 - CA265_RS19245 0.63 +0.6 4,781,619 - CA265_RS19245 0.65 +0.6 4,781,628 - CA265_RS19245 0.66 -0.3 4,781,638 + CA265_RS19245 0.68 -0.4 4,781,639 - CA265_RS19245 0.68 -1.1 4,781,643 + CA265_RS19245 0.69 -1.1 4,781,649 + CA265_RS19245 0.70 -0.0 4,781,649 + CA265_RS19245 0.70 -0.3 4,781,649 + CA265_RS19245 0.70 -0.7 4,781,650 - CA265_RS19245 0.70 -1.7 4,781,650 - CA265_RS19245 0.70 -0.6 4,781,693 + CA265_RS19245 0.77 -0.0 4,781,693 + CA265_RS19245 0.77 -1.1 4,781,694 - CA265_RS19245 0.77 -1.4 4,781,696 - CA265_RS19245 0.78 -2.7 4,781,721 + CA265_RS19245 0.82 -1.0 4,781,725 + CA265_RS19245 0.83 -1.4 4,781,819 - -1.6 4,781,827 + -0.7 4,781,828 - -0.4 4,781,836 - +0.2 4,781,912 + +1.0 4,782,019 + CA265_RS19250 0.14 +1.0 4,782,071 - CA265_RS19250 0.18 -0.2 4,782,071 - CA265_RS19250 0.18 -0.8 4,782,071 - CA265_RS19250 0.18 -2.1 4,782,071 - CA265_RS19250 0.18 -3.5 4,782,163 - CA265_RS19250 0.26 +0.4 4,782,176 + CA265_RS19250 0.27 -1.3 4,782,191 + CA265_RS19250 0.28 -0.0 4,782,195 - CA265_RS19250 0.29 -2.6 4,782,289 + CA265_RS19250 0.36 -0.7 4,782,289 + CA265_RS19250 0.36 -1.7 4,782,291 + CA265_RS19250 0.36 -0.7 4,782,291 + CA265_RS19250 0.36 -1.7 4,782,292 - CA265_RS19250 0.37 -1.3 4,782,338 + CA265_RS19250 0.40 -0.2 4,782,338 + CA265_RS19250 0.40 -0.2 4,782,383 + CA265_RS19250 0.44 +0.2 4,782,383 + CA265_RS19250 0.44 -2.0 4,782,428 + CA265_RS19250 0.48 +1.0 4,782,429 - CA265_RS19250 0.48 -1.0 4,782,463 - CA265_RS19250 0.51 -1.7 4,782,602 - CA265_RS19250 0.62 -1.7 4,782,663 - +1.4 4,782,668 - CA265_RS19250 0.67 -2.4 4,782,670 - CA265_RS19250 0.68 -1.0 4,782,777 + CA265_RS19250 0.76 -1.8 4,782,777 + CA265_RS19250 0.76 -2.4
Or see this region's nucleotide sequence