Experiment: R2A with A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS19180 and CA265_RS19185 are separated by 6 nucleotides CA265_RS19185 and CA265_RS19190 are separated by 45 nucleotides CA265_RS19190 and CA265_RS19195 overlap by 8 nucleotides
CA265_RS19180: CA265_RS19180 - gliding motility protein RemB, at 4,767,704 to 4,769,317
_RS19180
CA265_RS19185: CA265_RS19185 - polysaccharide biosynthesis protein, at 4,769,324 to 4,771,264
_RS19185
CA265_RS19190: CA265_RS19190 - transferase, at 4,771,310 to 4,771,858
_RS19190
CA265_RS19195: CA265_RS19195 - UDP-GlcNAc--UDP-phosphate GlcNAc-1-phosphate transferase, at 4,771,851 to 4,772,831
_RS19195
Position (kb)
4769
4770
4771
4772 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4768.363 kb on + strand, within CA265_RS19180 at 4768.408 kb on - strand, within CA265_RS19180 at 4768.408 kb on - strand, within CA265_RS19180 at 4768.412 kb on - strand, within CA265_RS19180 at 4768.509 kb on + strand, within CA265_RS19180 at 4768.510 kb on - strand, within CA265_RS19180 at 4768.565 kb on - strand, within CA265_RS19180 at 4768.586 kb on - strand, within CA265_RS19180 at 4768.586 kb on - strand, within CA265_RS19180 at 4768.588 kb on - strand, within CA265_RS19180 at 4768.636 kb on + strand, within CA265_RS19180 at 4768.648 kb on - strand, within CA265_RS19180 at 4768.651 kb on + strand, within CA265_RS19180 at 4768.667 kb on + strand, within CA265_RS19180 at 4768.670 kb on + strand, within CA265_RS19180 at 4768.731 kb on + strand, within CA265_RS19180 at 4768.731 kb on + strand, within CA265_RS19180 at 4768.732 kb on - strand, within CA265_RS19180 at 4768.732 kb on - strand, within CA265_RS19180 at 4768.755 kb on - strand, within CA265_RS19180 at 4768.755 kb on - strand, within CA265_RS19180 at 4768.763 kb on - strand, within CA265_RS19180 at 4768.767 kb on + strand, within CA265_RS19180 at 4768.768 kb on - strand, within CA265_RS19180 at 4768.843 kb on + strand, within CA265_RS19180 at 4768.844 kb on - strand, within CA265_RS19180 at 4768.847 kb on + strand, within CA265_RS19180 at 4768.848 kb on - strand, within CA265_RS19180 at 4768.886 kb on + strand, within CA265_RS19180 at 4768.886 kb on + strand, within CA265_RS19180 at 4768.940 kb on - strand, within CA265_RS19180 at 4768.940 kb on - strand, within CA265_RS19180 at 4769.007 kb on + strand, within CA265_RS19180 at 4769.016 kb on + strand, within CA265_RS19180 at 4769.099 kb on + strand, within CA265_RS19180 at 4769.138 kb on - strand, within CA265_RS19180 at 4769.230 kb on - strand at 4769.337 kb on - strand at 4769.337 kb on - strand at 4769.375 kb on - strand at 4769.375 kb on - strand at 4769.398 kb on + strand at 4769.420 kb on - strand at 4769.420 kb on - strand at 4769.451 kb on + strand at 4769.465 kb on + strand at 4769.495 kb on - strand at 4769.521 kb on + strand, within CA265_RS19185 at 4769.672 kb on - strand, within CA265_RS19185 at 4769.748 kb on - strand, within CA265_RS19185 at 4769.893 kb on + strand, within CA265_RS19185 at 4769.911 kb on + strand, within CA265_RS19185 at 4769.911 kb on + strand, within CA265_RS19185 at 4769.956 kb on - strand, within CA265_RS19185 at 4770.038 kb on - strand, within CA265_RS19185 at 4770.160 kb on - strand, within CA265_RS19185 at 4770.175 kb on - strand, within CA265_RS19185 at 4770.176 kb on + strand, within CA265_RS19185 at 4770.176 kb on + strand, within CA265_RS19185 at 4770.176 kb on + strand, within CA265_RS19185 at 4770.176 kb on + strand, within CA265_RS19185 at 4770.176 kb on + strand, within CA265_RS19185 at 4770.177 kb on - strand, within CA265_RS19185 at 4770.177 kb on - strand, within CA265_RS19185 at 4770.177 kb on - strand, within CA265_RS19185 at 4770.177 kb on - strand, within CA265_RS19185 at 4770.180 kb on + strand, within CA265_RS19185 at 4770.180 kb on + strand, within CA265_RS19185 at 4770.208 kb on + strand, within CA265_RS19185 at 4770.259 kb on + strand, within CA265_RS19185 at 4770.260 kb on - strand, within CA265_RS19185 at 4770.470 kb on + strand, within CA265_RS19185 at 4770.470 kb on + strand, within CA265_RS19185 at 4770.471 kb on - strand, within CA265_RS19185 at 4770.549 kb on - strand, within CA265_RS19185 at 4770.613 kb on + strand, within CA265_RS19185 at 4770.615 kb on + strand, within CA265_RS19185 at 4770.638 kb on + strand, within CA265_RS19185 at 4770.661 kb on - strand, within CA265_RS19185 at 4770.661 kb on - strand, within CA265_RS19185 at 4770.661 kb on - strand, within CA265_RS19185 at 4770.661 kb on - strand, within CA265_RS19185 at 4770.711 kb on - strand, within CA265_RS19185 at 4770.746 kb on - strand, within CA265_RS19185 at 4770.746 kb on - strand, within CA265_RS19185 at 4770.842 kb on - strand, within CA265_RS19185 at 4770.855 kb on + strand, within CA265_RS19185 at 4770.871 kb on + strand, within CA265_RS19185 at 4770.886 kb on - strand, within CA265_RS19185 at 4771.009 kb on + strand, within CA265_RS19185 at 4771.019 kb on + strand, within CA265_RS19185 at 4771.045 kb on + strand, within CA265_RS19185 at 4771.045 kb on + strand, within CA265_RS19185 at 4771.045 kb on + strand, within CA265_RS19185 at 4771.046 kb on - strand, within CA265_RS19185 at 4771.060 kb on + strand, within CA265_RS19185 at 4771.067 kb on - strand, within CA265_RS19185 at 4771.068 kb on + strand, within CA265_RS19185 at 4771.068 kb on + strand, within CA265_RS19185 at 4771.069 kb on - strand, within CA265_RS19185 at 4771.075 kb on + strand at 4771.078 kb on + strand at 4771.079 kb on - strand at 4771.099 kb on + strand at 4771.099 kb on + strand at 4771.100 kb on - strand at 4771.154 kb on + strand at 4771.166 kb on - strand at 4771.166 kb on - strand at 4771.267 kb on + strand at 4771.273 kb on - strand at 4771.293 kb on + strand at 4771.294 kb on - strand at 4771.300 kb on + strand at 4771.300 kb on + strand at 4771.301 kb on - strand at 4771.314 kb on + strand at 4771.314 kb on + strand at 4771.401 kb on - strand, within CA265_RS19190 at 4771.435 kb on - strand, within CA265_RS19190 at 4771.448 kb on + strand, within CA265_RS19190 at 4771.448 kb on + strand, within CA265_RS19190 at 4771.448 kb on + strand, within CA265_RS19190 at 4771.449 kb on - strand, within CA265_RS19190 at 4771.449 kb on - strand, within CA265_RS19190 at 4771.450 kb on + strand, within CA265_RS19190 at 4771.491 kb on - strand, within CA265_RS19190 at 4771.598 kb on - strand, within CA265_RS19190 at 4771.598 kb on - strand, within CA265_RS19190 at 4771.631 kb on + strand, within CA265_RS19190 at 4771.631 kb on + strand, within CA265_RS19190 at 4771.632 kb on - strand, within CA265_RS19190 at 4771.636 kb on + strand, within CA265_RS19190 at 4771.637 kb on - strand, within CA265_RS19190 at 4771.659 kb on - strand, within CA265_RS19190 at 4771.669 kb on + strand, within CA265_RS19190 at 4771.756 kb on + strand, within CA265_RS19190 at 4771.757 kb on - strand, within CA265_RS19190 at 4771.863 kb on + strand at 4771.864 kb on - strand at 4772.116 kb on - strand, within CA265_RS19195 at 4772.152 kb on + strand, within CA265_RS19195 at 4772.152 kb on + strand, within CA265_RS19195 at 4772.172 kb on - strand, within CA265_RS19195 at 4772.217 kb on + strand, within CA265_RS19195 at 4772.239 kb on - strand, within CA265_RS19195 at 4772.248 kb on - strand, within CA265_RS19195
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.02 mg/ml remove 4,768,363 + CA265_RS19180 0.41 -1.6 4,768,408 - CA265_RS19180 0.44 +0.2 4,768,408 - CA265_RS19180 0.44 -0.1 4,768,412 - CA265_RS19180 0.44 -1.9 4,768,509 + CA265_RS19180 0.50 -0.5 4,768,510 - CA265_RS19180 0.50 -1.7 4,768,565 - CA265_RS19180 0.53 -1.2 4,768,586 - CA265_RS19180 0.55 -0.3 4,768,586 - CA265_RS19180 0.55 -1.2 4,768,588 - CA265_RS19180 0.55 -1.9 4,768,636 + CA265_RS19180 0.58 -1.0 4,768,648 - CA265_RS19180 0.58 -1.0 4,768,651 + CA265_RS19180 0.59 -0.8 4,768,667 + CA265_RS19180 0.60 -1.5 4,768,670 + CA265_RS19180 0.60 +0.2 4,768,731 + CA265_RS19180 0.64 -0.7 4,768,731 + CA265_RS19180 0.64 +0.5 4,768,732 - CA265_RS19180 0.64 -0.3 4,768,732 - CA265_RS19180 0.64 -0.9 4,768,755 - CA265_RS19180 0.65 -0.6 4,768,755 - CA265_RS19180 0.65 -3.2 4,768,763 - CA265_RS19180 0.66 -2.2 4,768,767 + CA265_RS19180 0.66 -0.9 4,768,768 - CA265_RS19180 0.66 -0.7 4,768,843 + CA265_RS19180 0.71 -1.1 4,768,844 - CA265_RS19180 0.71 -0.0 4,768,847 + CA265_RS19180 0.71 -0.1 4,768,848 - CA265_RS19180 0.71 -0.5 4,768,886 + CA265_RS19180 0.73 -1.3 4,768,886 + CA265_RS19180 0.73 -0.3 4,768,940 - CA265_RS19180 0.77 -1.1 4,768,940 - CA265_RS19180 0.77 -1.7 4,769,007 + CA265_RS19180 0.81 -0.0 4,769,016 + CA265_RS19180 0.81 -2.2 4,769,099 + CA265_RS19180 0.86 -0.4 4,769,138 - CA265_RS19180 0.89 +0.5 4,769,230 - -0.2 4,769,337 - -0.1 4,769,337 - -0.9 4,769,375 - -1.6 4,769,375 - -1.6 4,769,398 + +0.2 4,769,420 - -1.8 4,769,420 - -2.1 4,769,451 + -2.8 4,769,465 + -1.6 4,769,495 - -1.9 4,769,521 + CA265_RS19185 0.10 -2.6 4,769,672 - CA265_RS19185 0.18 -3.1 4,769,748 - CA265_RS19185 0.22 -2.3 4,769,893 + CA265_RS19185 0.29 -0.5 4,769,911 + CA265_RS19185 0.30 -1.2 4,769,911 + CA265_RS19185 0.30 -1.0 4,769,956 - CA265_RS19185 0.33 -1.0 4,770,038 - CA265_RS19185 0.37 -2.1 4,770,160 - CA265_RS19185 0.43 -1.2 4,770,175 - CA265_RS19185 0.44 -1.8 4,770,176 + CA265_RS19185 0.44 -1.6 4,770,176 + CA265_RS19185 0.44 -0.8 4,770,176 + CA265_RS19185 0.44 -1.0 4,770,176 + CA265_RS19185 0.44 -3.1 4,770,176 + CA265_RS19185 0.44 -0.0 4,770,177 - CA265_RS19185 0.44 -0.6 4,770,177 - CA265_RS19185 0.44 -3.5 4,770,177 - CA265_RS19185 0.44 -1.3 4,770,177 - CA265_RS19185 0.44 -0.3 4,770,180 + CA265_RS19185 0.44 -0.0 4,770,180 + CA265_RS19185 0.44 -1.2 4,770,208 + CA265_RS19185 0.46 -2.4 4,770,259 + CA265_RS19185 0.48 -0.6 4,770,260 - CA265_RS19185 0.48 -0.5 4,770,470 + CA265_RS19185 0.59 +0.4 4,770,470 + CA265_RS19185 0.59 -0.1 4,770,471 - CA265_RS19185 0.59 -1.5 4,770,549 - CA265_RS19185 0.63 -1.4 4,770,613 + CA265_RS19185 0.66 -2.1 4,770,615 + CA265_RS19185 0.67 -2.9 4,770,638 + CA265_RS19185 0.68 -1.4 4,770,661 - CA265_RS19185 0.69 -0.2 4,770,661 - CA265_RS19185 0.69 -1.6 4,770,661 - CA265_RS19185 0.69 +0.2 4,770,661 - CA265_RS19185 0.69 +0.4 4,770,711 - CA265_RS19185 0.71 -0.6 4,770,746 - CA265_RS19185 0.73 -1.0 4,770,746 - CA265_RS19185 0.73 +0.4 4,770,842 - CA265_RS19185 0.78 -2.5 4,770,855 + CA265_RS19185 0.79 -3.2 4,770,871 + CA265_RS19185 0.80 +0.2 4,770,886 - CA265_RS19185 0.80 -2.7 4,771,009 + CA265_RS19185 0.87 -1.5 4,771,019 + CA265_RS19185 0.87 -1.9 4,771,045 + CA265_RS19185 0.89 -4.2 4,771,045 + CA265_RS19185 0.89 +1.0 4,771,045 + CA265_RS19185 0.89 +0.4 4,771,046 - CA265_RS19185 0.89 -2.5 4,771,060 + CA265_RS19185 0.89 -1.0 4,771,067 - CA265_RS19185 0.90 -1.4 4,771,068 + CA265_RS19185 0.90 -2.4 4,771,068 + CA265_RS19185 0.90 -1.6 4,771,069 - CA265_RS19185 0.90 -4.1 4,771,075 + -2.1 4,771,078 + -1.1 4,771,079 - -1.2 4,771,099 + -0.8 4,771,099 + -1.3 4,771,100 - -0.6 4,771,154 + -0.5 4,771,166 - -3.3 4,771,166 - -1.0 4,771,267 + -1.6 4,771,273 - -0.7 4,771,293 + -2.9 4,771,294 - +0.1 4,771,300 + -0.8 4,771,300 + -2.5 4,771,301 - +0.1 4,771,314 + -0.5 4,771,314 + -1.3 4,771,401 - CA265_RS19190 0.17 -0.1 4,771,435 - CA265_RS19190 0.23 +0.7 4,771,448 + CA265_RS19190 0.25 -0.8 4,771,448 + CA265_RS19190 0.25 -0.3 4,771,448 + CA265_RS19190 0.25 -0.4 4,771,449 - CA265_RS19190 0.25 +0.3 4,771,449 - CA265_RS19190 0.25 +0.4 4,771,450 + CA265_RS19190 0.26 +0.7 4,771,491 - CA265_RS19190 0.33 +0.5 4,771,598 - CA265_RS19190 0.52 +0.8 4,771,598 - CA265_RS19190 0.52 +0.3 4,771,631 + CA265_RS19190 0.58 +2.0 4,771,631 + CA265_RS19190 0.58 -0.2 4,771,632 - CA265_RS19190 0.59 -0.7 4,771,636 + CA265_RS19190 0.59 -0.9 4,771,637 - CA265_RS19190 0.60 +1.1 4,771,659 - CA265_RS19190 0.64 -0.3 4,771,669 + CA265_RS19190 0.65 +0.2 4,771,756 + CA265_RS19190 0.81 -0.1 4,771,757 - CA265_RS19190 0.81 -0.7 4,771,863 + -1.8 4,771,864 - +0.7 4,772,116 - CA265_RS19195 0.27 -0.6 4,772,152 + CA265_RS19195 0.31 -2.1 4,772,152 + CA265_RS19195 0.31 -1.0 4,772,172 - CA265_RS19195 0.33 -0.2 4,772,217 + CA265_RS19195 0.37 -0.5 4,772,239 - CA265_RS19195 0.40 -3.6 4,772,248 - CA265_RS19195 0.40 -2.3
Or see this region's nucleotide sequence