Experiment: R2A with A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS18545 and CA265_RS18550 are separated by 1 nucleotides CA265_RS18550 and CA265_RS18555 overlap by 22 nucleotides CA265_RS18555 and CA265_RS18560 are separated by 409 nucleotides
CA265_RS18545: CA265_RS18545 - argininosuccinate synthase, at 4,609,313 to 4,610,500
_RS18545
CA265_RS18550: CA265_RS18550 - N-acetyltransferase, at 4,610,502 to 4,611,200
_RS18550
CA265_RS18555: CA265_RS18555 - hypothetical protein, at 4,611,179 to 4,611,373
_RS18555
CA265_RS18560: CA265_RS18560 - hypothetical protein, at 4,611,783 to 4,612,484
_RS18560
Position (kb)
4610
4611
4612 Strain fitness (log2 ratio)
-1
0
1
2 at 4609.608 kb on - strand, within CA265_RS18545 at 4609.672 kb on + strand, within CA265_RS18545 at 4609.857 kb on - strand, within CA265_RS18545 at 4609.857 kb on - strand, within CA265_RS18545 at 4609.862 kb on + strand, within CA265_RS18545 at 4610.005 kb on - strand, within CA265_RS18545 at 4610.005 kb on - strand, within CA265_RS18545 at 4610.066 kb on - strand, within CA265_RS18545 at 4610.095 kb on + strand, within CA265_RS18545 at 4610.096 kb on - strand, within CA265_RS18545 at 4610.104 kb on + strand, within CA265_RS18545 at 4610.113 kb on + strand, within CA265_RS18545 at 4610.187 kb on + strand, within CA265_RS18545 at 4610.188 kb on - strand, within CA265_RS18545 at 4610.229 kb on - strand, within CA265_RS18545 at 4610.295 kb on + strand, within CA265_RS18545 at 4610.296 kb on - strand, within CA265_RS18545 at 4610.395 kb on + strand at 4610.420 kb on + strand at 4610.420 kb on + strand at 4610.443 kb on - strand at 4610.462 kb on - strand at 4610.520 kb on - strand at 4610.647 kb on - strand, within CA265_RS18550 at 4610.800 kb on + strand, within CA265_RS18550 at 4610.992 kb on - strand, within CA265_RS18550 at 4611.227 kb on - strand, within CA265_RS18555 at 4611.291 kb on - strand, within CA265_RS18555 at 4611.351 kb on - strand, within CA265_RS18555 at 4611.483 kb on - strand at 4611.490 kb on - strand at 4611.493 kb on - strand at 4611.563 kb on + strand at 4611.609 kb on + strand at 4611.609 kb on + strand at 4611.694 kb on + strand at 4611.744 kb on - strand at 4611.782 kb on + strand at 4611.783 kb on - strand at 4611.884 kb on + strand, within CA265_RS18560 at 4611.885 kb on - strand, within CA265_RS18560 at 4611.889 kb on + strand, within CA265_RS18560 at 4611.898 kb on + strand, within CA265_RS18560 at 4611.899 kb on - strand, within CA265_RS18560 at 4611.934 kb on - strand, within CA265_RS18560 at 4611.942 kb on + strand, within CA265_RS18560 at 4611.959 kb on - strand, within CA265_RS18560 at 4611.959 kb on - strand, within CA265_RS18560 at 4611.976 kb on + strand, within CA265_RS18560 at 4611.977 kb on - strand, within CA265_RS18560 at 4611.999 kb on + strand, within CA265_RS18560 at 4612.085 kb on - strand, within CA265_RS18560 at 4612.098 kb on + strand, within CA265_RS18560 at 4612.098 kb on + strand, within CA265_RS18560 at 4612.140 kb on - strand, within CA265_RS18560 at 4612.140 kb on - strand, within CA265_RS18560 at 4612.143 kb on + strand, within CA265_RS18560 at 4612.188 kb on + strand, within CA265_RS18560 at 4612.188 kb on + strand, within CA265_RS18560
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.02 mg/ml remove 4,609,608 - CA265_RS18545 0.25 +0.7 4,609,672 + CA265_RS18545 0.30 +1.5 4,609,857 - CA265_RS18545 0.46 +2.7 4,609,857 - CA265_RS18545 0.46 +0.3 4,609,862 + CA265_RS18545 0.46 +0.7 4,610,005 - CA265_RS18545 0.58 -0.2 4,610,005 - CA265_RS18545 0.58 -0.3 4,610,066 - CA265_RS18545 0.63 +0.1 4,610,095 + CA265_RS18545 0.66 +1.2 4,610,096 - CA265_RS18545 0.66 -0.9 4,610,104 + CA265_RS18545 0.67 +0.9 4,610,113 + CA265_RS18545 0.67 +0.2 4,610,187 + CA265_RS18545 0.74 -0.5 4,610,188 - CA265_RS18545 0.74 -0.2 4,610,229 - CA265_RS18545 0.77 +0.3 4,610,295 + CA265_RS18545 0.83 +0.5 4,610,296 - CA265_RS18545 0.83 -0.8 4,610,395 + +1.0 4,610,420 + +0.6 4,610,420 + -1.1 4,610,443 - +0.7 4,610,462 - +0.6 4,610,520 - +1.1 4,610,647 - CA265_RS18550 0.21 +0.1 4,610,800 + CA265_RS18550 0.43 -0.5 4,610,992 - CA265_RS18550 0.70 -1.1 4,611,227 - CA265_RS18555 0.25 -0.9 4,611,291 - CA265_RS18555 0.57 -0.2 4,611,351 - CA265_RS18555 0.88 -0.5 4,611,483 - -0.2 4,611,490 - -0.4 4,611,493 - +0.5 4,611,563 + -0.9 4,611,609 + -0.6 4,611,609 + +0.1 4,611,694 + -0.2 4,611,744 - -1.2 4,611,782 + +1.9 4,611,783 - -0.7 4,611,884 + CA265_RS18560 0.14 -0.2 4,611,885 - CA265_RS18560 0.15 -0.2 4,611,889 + CA265_RS18560 0.15 -0.4 4,611,898 + CA265_RS18560 0.16 -0.2 4,611,899 - CA265_RS18560 0.17 +0.3 4,611,934 - CA265_RS18560 0.22 +0.1 4,611,942 + CA265_RS18560 0.23 -0.2 4,611,959 - CA265_RS18560 0.25 -0.9 4,611,959 - CA265_RS18560 0.25 +0.6 4,611,976 + CA265_RS18560 0.27 -1.5 4,611,977 - CA265_RS18560 0.28 -0.7 4,611,999 + CA265_RS18560 0.31 -0.3 4,612,085 - CA265_RS18560 0.43 -1.2 4,612,098 + CA265_RS18560 0.45 +0.1 4,612,098 + CA265_RS18560 0.45 -0.2 4,612,140 - CA265_RS18560 0.51 +0.7 4,612,140 - CA265_RS18560 0.51 -0.6 4,612,143 + CA265_RS18560 0.51 -0.7 4,612,188 + CA265_RS18560 0.58 +0.1 4,612,188 + CA265_RS18560 0.58 +0.2
Or see this region's nucleotide sequence