Experiment: R2A with A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS18230 and CA265_RS18235 are separated by 3 nucleotides CA265_RS18235 and CA265_RS18240 are separated by 228 nucleotides
CA265_RS18230: CA265_RS18230 - two-component sensor histidine kinase, at 4,545,186 to 4,546,859
_RS18230
CA265_RS18235: CA265_RS18235 - peptidase, at 4,546,863 to 4,548,746
_RS18235
CA265_RS18240: CA265_RS18240 - hypothetical protein, at 4,548,975 to 4,550,798
_RS18240
Position (kb)
4546
4547
4548
4549 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4545.872 kb on + strand, within CA265_RS18230 at 4545.926 kb on - strand, within CA265_RS18230 at 4545.928 kb on - strand, within CA265_RS18230 at 4546.013 kb on + strand, within CA265_RS18230 at 4546.014 kb on - strand, within CA265_RS18230 at 4546.089 kb on + strand, within CA265_RS18230 at 4546.131 kb on - strand, within CA265_RS18230 at 4546.144 kb on - strand, within CA265_RS18230 at 4546.220 kb on + strand, within CA265_RS18230 at 4546.229 kb on - strand, within CA265_RS18230 at 4546.236 kb on + strand, within CA265_RS18230 at 4546.237 kb on - strand, within CA265_RS18230 at 4546.256 kb on + strand, within CA265_RS18230 at 4546.330 kb on - strand, within CA265_RS18230 at 4546.430 kb on - strand, within CA265_RS18230 at 4546.582 kb on - strand, within CA265_RS18230 at 4546.614 kb on - strand, within CA265_RS18230 at 4546.623 kb on + strand, within CA265_RS18230 at 4546.636 kb on + strand, within CA265_RS18230 at 4546.639 kb on + strand, within CA265_RS18230 at 4546.678 kb on + strand, within CA265_RS18230 at 4546.679 kb on - strand, within CA265_RS18230 at 4546.688 kb on + strand, within CA265_RS18230 at 4546.719 kb on + strand at 4546.720 kb on - strand at 4546.749 kb on - strand at 4546.751 kb on - strand at 4546.779 kb on - strand at 4546.909 kb on + strand at 4546.969 kb on + strand at 4546.974 kb on - strand at 4547.030 kb on + strand at 4547.114 kb on + strand, within CA265_RS18235 at 4547.155 kb on + strand, within CA265_RS18235 at 4547.155 kb on + strand, within CA265_RS18235 at 4547.155 kb on + strand, within CA265_RS18235 at 4547.156 kb on - strand, within CA265_RS18235 at 4547.205 kb on - strand, within CA265_RS18235 at 4547.217 kb on + strand, within CA265_RS18235 at 4547.282 kb on + strand, within CA265_RS18235 at 4547.285 kb on - strand, within CA265_RS18235 at 4547.356 kb on - strand, within CA265_RS18235 at 4547.413 kb on + strand, within CA265_RS18235 at 4547.413 kb on + strand, within CA265_RS18235 at 4547.428 kb on + strand, within CA265_RS18235 at 4547.435 kb on + strand, within CA265_RS18235 at 4547.446 kb on + strand, within CA265_RS18235 at 4547.502 kb on - strand, within CA265_RS18235 at 4547.502 kb on - strand, within CA265_RS18235 at 4547.504 kb on - strand, within CA265_RS18235 at 4547.534 kb on + strand, within CA265_RS18235 at 4547.535 kb on - strand, within CA265_RS18235 at 4547.535 kb on - strand, within CA265_RS18235 at 4547.557 kb on - strand, within CA265_RS18235 at 4547.593 kb on + strand, within CA265_RS18235 at 4547.678 kb on + strand, within CA265_RS18235 at 4547.679 kb on - strand, within CA265_RS18235 at 4547.714 kb on + strand, within CA265_RS18235 at 4547.714 kb on + strand, within CA265_RS18235 at 4547.718 kb on + strand, within CA265_RS18235 at 4547.718 kb on + strand, within CA265_RS18235 at 4547.723 kb on - strand, within CA265_RS18235 at 4547.723 kb on - strand, within CA265_RS18235 at 4547.837 kb on - strand, within CA265_RS18235 at 4547.850 kb on - strand at 4547.940 kb on + strand, within CA265_RS18235 at 4547.951 kb on + strand, within CA265_RS18235 at 4548.018 kb on - strand, within CA265_RS18235 at 4548.028 kb on - strand, within CA265_RS18235 at 4548.043 kb on + strand, within CA265_RS18235 at 4548.043 kb on + strand, within CA265_RS18235 at 4548.044 kb on - strand, within CA265_RS18235 at 4548.044 kb on - strand, within CA265_RS18235 at 4548.048 kb on + strand, within CA265_RS18235 at 4548.048 kb on + strand, within CA265_RS18235 at 4548.048 kb on + strand, within CA265_RS18235 at 4548.048 kb on + strand, within CA265_RS18235 at 4548.049 kb on - strand, within CA265_RS18235 at 4548.108 kb on - strand, within CA265_RS18235 at 4548.249 kb on - strand, within CA265_RS18235 at 4548.258 kb on - strand, within CA265_RS18235 at 4548.324 kb on - strand, within CA265_RS18235 at 4548.326 kb on + strand, within CA265_RS18235 at 4548.327 kb on - strand, within CA265_RS18235 at 4548.332 kb on + strand, within CA265_RS18235 at 4548.342 kb on + strand, within CA265_RS18235 at 4548.355 kb on - strand, within CA265_RS18235 at 4548.368 kb on + strand, within CA265_RS18235 at 4548.401 kb on + strand, within CA265_RS18235 at 4548.402 kb on - strand, within CA265_RS18235 at 4548.438 kb on - strand, within CA265_RS18235 at 4548.447 kb on - strand, within CA265_RS18235 at 4548.467 kb on - strand, within CA265_RS18235 at 4548.480 kb on - strand, within CA265_RS18235 at 4548.480 kb on - strand, within CA265_RS18235 at 4548.674 kb on + strand at 4548.675 kb on - strand at 4548.675 kb on - strand at 4548.677 kb on + strand at 4548.695 kb on + strand at 4548.750 kb on + strand at 4548.776 kb on - strand at 4548.822 kb on - strand at 4548.836 kb on - strand at 4548.856 kb on - strand at 4548.888 kb on + strand at 4548.888 kb on + strand at 4548.936 kb on + strand at 4548.963 kb on - strand at 4548.974 kb on + strand at 4548.997 kb on + strand at 4549.081 kb on + strand at 4549.086 kb on + strand at 4549.087 kb on - strand at 4549.090 kb on - strand at 4549.090 kb on - strand at 4549.124 kb on - strand at 4549.161 kb on + strand, within CA265_RS18240 at 4549.170 kb on - strand, within CA265_RS18240 at 4549.182 kb on - strand, within CA265_RS18240 at 4549.182 kb on - strand, within CA265_RS18240 at 4549.270 kb on + strand, within CA265_RS18240 at 4549.276 kb on + strand, within CA265_RS18240 at 4549.500 kb on - strand, within CA265_RS18240 at 4549.508 kb on + strand, within CA265_RS18240 at 4549.509 kb on - strand, within CA265_RS18240 at 4549.684 kb on + strand, within CA265_RS18240 at 4549.698 kb on + strand, within CA265_RS18240 at 4549.740 kb on - strand, within CA265_RS18240
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.02 mg/ml remove 4,545,872 + CA265_RS18230 0.41 +0.1 4,545,926 - CA265_RS18230 0.44 -1.5 4,545,928 - CA265_RS18230 0.44 -2.5 4,546,013 + CA265_RS18230 0.49 -1.4 4,546,014 - CA265_RS18230 0.49 -1.5 4,546,089 + CA265_RS18230 0.54 -0.1 4,546,131 - CA265_RS18230 0.56 -0.2 4,546,144 - CA265_RS18230 0.57 -1.1 4,546,220 + CA265_RS18230 0.62 +0.9 4,546,229 - CA265_RS18230 0.62 +0.9 4,546,236 + CA265_RS18230 0.63 +0.6 4,546,237 - CA265_RS18230 0.63 -2.4 4,546,256 + CA265_RS18230 0.64 -0.2 4,546,330 - CA265_RS18230 0.68 -1.0 4,546,430 - CA265_RS18230 0.74 -1.6 4,546,582 - CA265_RS18230 0.83 -2.4 4,546,614 - CA265_RS18230 0.85 -1.1 4,546,623 + CA265_RS18230 0.86 +0.9 4,546,636 + CA265_RS18230 0.87 -0.1 4,546,639 + CA265_RS18230 0.87 +1.7 4,546,678 + CA265_RS18230 0.89 -0.1 4,546,679 - CA265_RS18230 0.89 -1.2 4,546,688 + CA265_RS18230 0.90 -0.0 4,546,719 + -0.3 4,546,720 - -2.0 4,546,749 - -0.1 4,546,751 - -0.1 4,546,779 - -0.3 4,546,909 + -1.5 4,546,969 + +0.4 4,546,974 - -1.3 4,547,030 + -0.3 4,547,114 + CA265_RS18235 0.13 +0.8 4,547,155 + CA265_RS18235 0.15 +0.6 4,547,155 + CA265_RS18235 0.15 -0.1 4,547,155 + CA265_RS18235 0.15 +1.1 4,547,156 - CA265_RS18235 0.16 -1.5 4,547,205 - CA265_RS18235 0.18 -2.5 4,547,217 + CA265_RS18235 0.19 -2.6 4,547,282 + CA265_RS18235 0.22 +0.3 4,547,285 - CA265_RS18235 0.22 -0.5 4,547,356 - CA265_RS18235 0.26 -0.0 4,547,413 + CA265_RS18235 0.29 -1.3 4,547,413 + CA265_RS18235 0.29 -1.0 4,547,428 + CA265_RS18235 0.30 -0.1 4,547,435 + CA265_RS18235 0.30 -0.8 4,547,446 + CA265_RS18235 0.31 -0.1 4,547,502 - CA265_RS18235 0.34 -2.0 4,547,502 - CA265_RS18235 0.34 -2.2 4,547,504 - CA265_RS18235 0.34 +0.3 4,547,534 + CA265_RS18235 0.36 +0.0 4,547,535 - CA265_RS18235 0.36 -3.5 4,547,535 - CA265_RS18235 0.36 -1.8 4,547,557 - CA265_RS18235 0.37 +0.3 4,547,593 + CA265_RS18235 0.39 -0.8 4,547,678 + CA265_RS18235 0.43 -0.2 4,547,679 - CA265_RS18235 0.43 -1.7 4,547,714 + CA265_RS18235 0.45 +0.2 4,547,714 + CA265_RS18235 0.45 +0.3 4,547,718 + CA265_RS18235 0.45 -2.4 4,547,718 + CA265_RS18235 0.45 -1.7 4,547,723 - CA265_RS18235 0.46 -1.3 4,547,723 - CA265_RS18235 0.46 -2.3 4,547,837 - CA265_RS18235 0.52 -2.7 4,547,850 - +1.3 4,547,940 + CA265_RS18235 0.57 -0.5 4,547,951 + CA265_RS18235 0.58 -2.0 4,548,018 - CA265_RS18235 0.61 -3.2 4,548,028 - CA265_RS18235 0.62 -2.3 4,548,043 + CA265_RS18235 0.63 +0.6 4,548,043 + CA265_RS18235 0.63 +0.4 4,548,044 - CA265_RS18235 0.63 -2.7 4,548,044 - CA265_RS18235 0.63 -2.9 4,548,048 + CA265_RS18235 0.63 +1.5 4,548,048 + CA265_RS18235 0.63 -1.8 4,548,048 + CA265_RS18235 0.63 -2.3 4,548,048 + CA265_RS18235 0.63 -1.2 4,548,049 - CA265_RS18235 0.63 -1.4 4,548,108 - CA265_RS18235 0.66 -2.6 4,548,249 - CA265_RS18235 0.74 -0.6 4,548,258 - CA265_RS18235 0.74 -2.1 4,548,324 - CA265_RS18235 0.78 -0.9 4,548,326 + CA265_RS18235 0.78 +0.0 4,548,327 - CA265_RS18235 0.78 -1.6 4,548,332 + CA265_RS18235 0.78 -0.5 4,548,342 + CA265_RS18235 0.79 -0.8 4,548,355 - CA265_RS18235 0.79 -0.4 4,548,368 + CA265_RS18235 0.80 +0.4 4,548,401 + CA265_RS18235 0.82 +0.5 4,548,402 - CA265_RS18235 0.82 -2.1 4,548,438 - CA265_RS18235 0.84 -1.1 4,548,447 - CA265_RS18235 0.84 -2.2 4,548,467 - CA265_RS18235 0.85 -0.7 4,548,480 - CA265_RS18235 0.86 -2.1 4,548,480 - CA265_RS18235 0.86 -3.0 4,548,674 + -0.4 4,548,675 - -1.8 4,548,675 - +0.3 4,548,677 + +0.9 4,548,695 + -0.4 4,548,750 + -0.1 4,548,776 - +0.7 4,548,822 - +0.6 4,548,836 - -0.3 4,548,856 - +0.2 4,548,888 + -0.6 4,548,888 + +0.5 4,548,936 + +0.9 4,548,963 - +0.2 4,548,974 + -0.1 4,548,997 + -0.7 4,549,081 + +0.3 4,549,086 + +1.0 4,549,087 - +0.1 4,549,090 - +1.1 4,549,090 - +0.3 4,549,124 - -0.1 4,549,161 + CA265_RS18240 0.10 +0.7 4,549,170 - CA265_RS18240 0.11 +0.3 4,549,182 - CA265_RS18240 0.11 -0.6 4,549,182 - CA265_RS18240 0.11 +0.2 4,549,270 + CA265_RS18240 0.16 -0.2 4,549,276 + CA265_RS18240 0.17 -1.7 4,549,500 - CA265_RS18240 0.29 +0.4 4,549,508 + CA265_RS18240 0.29 -0.1 4,549,509 - CA265_RS18240 0.29 -0.6 4,549,684 + CA265_RS18240 0.39 -0.3 4,549,698 + CA265_RS18240 0.40 +0.7 4,549,740 - CA265_RS18240 0.42 +0.1
Or see this region's nucleotide sequence