Experiment: R2A with A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS18020 and CA265_RS18025 are separated by 41 nucleotides CA265_RS18025 and CA265_RS18030 are separated by 155 nucleotides CA265_RS18030 and CA265_RS18035 are separated by 490 nucleotides CA265_RS18035 and CA265_RS18040 are separated by 57 nucleotides
CA265_RS18020: CA265_RS18020 - hypothetical protein, at 4,502,665 to 4,503,642
_RS18020
CA265_RS18025: CA265_RS18025 - crossover junction endodeoxyribonuclease RuvC, at 4,503,684 to 4,504,271
_RS18025
CA265_RS18030: CA265_RS18030 - hypothetical protein, at 4,504,427 to 4,505,299
_RS18030
CA265_RS18035: CA265_RS18035 - hypothetical protein, at 4,505,790 to 4,506,008
_RS18035
CA265_RS18040: CA265_RS18040 - 5'-nucleotidase, lipoprotein e(P4) family, at 4,506,066 to 4,506,830
_RS18040
Position (kb)
4504
4505
4506 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4503.510 kb on + strand, within CA265_RS18020 at 4503.666 kb on - strand at 4503.674 kb on + strand at 4503.682 kb on + strand at 4503.682 kb on + strand at 4503.682 kb on + strand at 4503.940 kb on + strand at 4504.339 kb on - strand at 4504.400 kb on + strand at 4504.476 kb on + strand at 4504.481 kb on + strand at 4504.514 kb on + strand at 4504.523 kb on + strand, within CA265_RS18030 at 4504.528 kb on + strand, within CA265_RS18030 at 4504.532 kb on + strand, within CA265_RS18030 at 4504.534 kb on + strand, within CA265_RS18030 at 4504.541 kb on + strand, within CA265_RS18030 at 4504.617 kb on + strand, within CA265_RS18030 at 4504.737 kb on + strand, within CA265_RS18030 at 4504.748 kb on - strand, within CA265_RS18030 at 4504.835 kb on + strand, within CA265_RS18030 at 4504.840 kb on + strand, within CA265_RS18030 at 4504.840 kb on + strand, within CA265_RS18030 at 4504.851 kb on + strand, within CA265_RS18030 at 4504.852 kb on - strand, within CA265_RS18030 at 4504.939 kb on + strand, within CA265_RS18030 at 4505.177 kb on - strand, within CA265_RS18030 at 4505.230 kb on - strand at 4505.249 kb on - strand at 4505.279 kb on - strand at 4505.288 kb on - strand at 4505.322 kb on - strand at 4505.398 kb on + strand at 4505.399 kb on - strand at 4505.411 kb on - strand at 4505.417 kb on - strand at 4505.470 kb on - strand at 4505.470 kb on - strand at 4505.470 kb on - strand at 4505.470 kb on - strand at 4505.506 kb on + strand at 4505.579 kb on + strand at 4505.580 kb on - strand at 4505.580 kb on - strand at 4505.653 kb on + strand at 4505.782 kb on + strand at 4505.792 kb on - strand at 4505.828 kb on + strand, within CA265_RS18035 at 4505.828 kb on + strand, within CA265_RS18035 at 4505.828 kb on + strand, within CA265_RS18035 at 4505.863 kb on - strand, within CA265_RS18035 at 4505.863 kb on - strand, within CA265_RS18035 at 4505.900 kb on - strand, within CA265_RS18035 at 4505.904 kb on - strand, within CA265_RS18035 at 4505.991 kb on + strand at 4505.992 kb on - strand at 4505.992 kb on - strand at 4506.021 kb on + strand at 4506.044 kb on + strand at 4506.044 kb on + strand at 4506.045 kb on - strand at 4506.175 kb on - strand, within CA265_RS18040 at 4506.181 kb on + strand, within CA265_RS18040 at 4506.182 kb on - strand, within CA265_RS18040 at 4506.294 kb on - strand, within CA265_RS18040 at 4506.294 kb on - strand, within CA265_RS18040 at 4506.294 kb on - strand, within CA265_RS18040
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.02 mg/ml remove 4,503,510 + CA265_RS18020 0.86 -1.4 4,503,666 - +0.3 4,503,674 + +0.7 4,503,682 + -1.1 4,503,682 + -1.2 4,503,682 + -0.9 4,503,940 + +2.6 4,504,339 - -0.5 4,504,400 + +0.6 4,504,476 + -0.2 4,504,481 + -0.3 4,504,514 + +0.7 4,504,523 + CA265_RS18030 0.11 +0.0 4,504,528 + CA265_RS18030 0.12 -0.2 4,504,532 + CA265_RS18030 0.12 +0.5 4,504,534 + CA265_RS18030 0.12 -0.7 4,504,541 + CA265_RS18030 0.13 -0.8 4,504,617 + CA265_RS18030 0.22 +0.6 4,504,737 + CA265_RS18030 0.36 -0.1 4,504,748 - CA265_RS18030 0.37 +0.6 4,504,835 + CA265_RS18030 0.47 +0.5 4,504,840 + CA265_RS18030 0.47 -0.8 4,504,840 + CA265_RS18030 0.47 -0.2 4,504,851 + CA265_RS18030 0.49 -1.3 4,504,852 - CA265_RS18030 0.49 -0.8 4,504,939 + CA265_RS18030 0.59 +0.1 4,505,177 - CA265_RS18030 0.86 +0.4 4,505,230 - -0.6 4,505,249 - -1.3 4,505,279 - -1.5 4,505,288 - +0.9 4,505,322 - -0.6 4,505,398 + -0.8 4,505,399 - -0.2 4,505,411 - -1.0 4,505,417 - -0.2 4,505,470 - -0.8 4,505,470 - -0.6 4,505,470 - +0.1 4,505,470 - -0.1 4,505,506 + -0.4 4,505,579 + -0.2 4,505,580 - -1.1 4,505,580 - -2.1 4,505,653 + -0.3 4,505,782 + -0.7 4,505,792 - +0.7 4,505,828 + CA265_RS18035 0.17 +1.0 4,505,828 + CA265_RS18035 0.17 -0.1 4,505,828 + CA265_RS18035 0.17 -0.5 4,505,863 - CA265_RS18035 0.33 -0.4 4,505,863 - CA265_RS18035 0.33 +1.3 4,505,900 - CA265_RS18035 0.50 +0.2 4,505,904 - CA265_RS18035 0.52 -0.1 4,505,991 + -0.1 4,505,992 - -0.0 4,505,992 - +0.7 4,506,021 + -1.1 4,506,044 + -0.6 4,506,044 + +0.2 4,506,045 - -0.5 4,506,175 - CA265_RS18040 0.14 -1.1 4,506,181 + CA265_RS18040 0.15 +0.7 4,506,182 - CA265_RS18040 0.15 +0.0 4,506,294 - CA265_RS18040 0.30 -0.2 4,506,294 - CA265_RS18040 0.30 -0.2 4,506,294 - CA265_RS18040 0.30 +1.0
Or see this region's nucleotide sequence