Experiment: R2A with A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS17325 and CA265_RS17330 are separated by 219 nucleotides CA265_RS17330 and CA265_RS17335 are separated by 90 nucleotides CA265_RS17335 and CA265_RS17340 overlap by 17 nucleotides
CA265_RS17325: CA265_RS17325 - deaminase, at 4,329,027 to 4,329,605
_RS17325
CA265_RS17330: CA265_RS17330 - hypothetical protein, at 4,329,825 to 4,330,139
_RS17330
CA265_RS17335: CA265_RS17335 - hypothetical protein, at 4,330,230 to 4,330,745
_RS17335
CA265_RS17340: CA265_RS17340 - hypothetical protein, at 4,330,729 to 4,331,283
_RS17340
Position (kb)
4329
4330
4331 Strain fitness (log2 ratio)
-2
-1
0
1 at 4328.904 kb on - strand at 4328.909 kb on + strand at 4328.909 kb on + strand at 4328.909 kb on + strand at 4328.910 kb on - strand at 4328.910 kb on - strand at 4328.910 kb on - strand at 4328.980 kb on + strand at 4329.049 kb on - strand at 4329.053 kb on - strand at 4329.081 kb on - strand at 4329.099 kb on + strand, within CA265_RS17325 at 4329.169 kb on + strand, within CA265_RS17325 at 4329.171 kb on + strand, within CA265_RS17325 at 4329.171 kb on + strand, within CA265_RS17325 at 4329.172 kb on - strand, within CA265_RS17325 at 4329.172 kb on - strand, within CA265_RS17325 at 4329.175 kb on - strand, within CA265_RS17325 at 4329.175 kb on - strand, within CA265_RS17325 at 4329.177 kb on + strand, within CA265_RS17325 at 4329.178 kb on - strand, within CA265_RS17325 at 4329.178 kb on - strand, within CA265_RS17325 at 4329.181 kb on - strand, within CA265_RS17325 at 4329.290 kb on + strand, within CA265_RS17325 at 4329.293 kb on + strand, within CA265_RS17325 at 4329.293 kb on + strand, within CA265_RS17325 at 4329.351 kb on + strand, within CA265_RS17325 at 4329.352 kb on - strand, within CA265_RS17325 at 4329.356 kb on - strand, within CA265_RS17325 at 4329.369 kb on - strand, within CA265_RS17325 at 4329.392 kb on + strand, within CA265_RS17325 at 4329.491 kb on + strand, within CA265_RS17325 at 4329.579 kb on - strand at 4329.579 kb on - strand at 4329.638 kb on + strand at 4329.639 kb on - strand at 4329.645 kb on + strand at 4329.645 kb on + strand at 4329.679 kb on + strand at 4329.705 kb on + strand at 4329.714 kb on + strand at 4329.787 kb on - strand at 4329.804 kb on - strand at 4329.981 kb on - strand, within CA265_RS17330 at 4329.993 kb on - strand, within CA265_RS17330 at 4330.065 kb on - strand, within CA265_RS17330 at 4330.074 kb on + strand, within CA265_RS17330 at 4330.075 kb on - strand, within CA265_RS17330 at 4330.140 kb on + strand at 4330.151 kb on - strand at 4330.170 kb on + strand at 4330.170 kb on + strand at 4330.170 kb on + strand at 4330.171 kb on - strand at 4330.171 kb on - strand at 4330.172 kb on + strand at 4330.173 kb on - strand at 4330.206 kb on - strand at 4330.224 kb on + strand at 4330.229 kb on - strand at 4330.267 kb on - strand at 4330.302 kb on - strand, within CA265_RS17335 at 4330.328 kb on - strand, within CA265_RS17335 at 4330.338 kb on - strand, within CA265_RS17335 at 4330.343 kb on + strand, within CA265_RS17335 at 4330.344 kb on - strand, within CA265_RS17335 at 4330.363 kb on - strand, within CA265_RS17335 at 4330.371 kb on + strand, within CA265_RS17335 at 4330.371 kb on + strand, within CA265_RS17335 at 4330.418 kb on - strand, within CA265_RS17335 at 4330.419 kb on + strand, within CA265_RS17335 at 4330.419 kb on + strand, within CA265_RS17335 at 4330.420 kb on - strand, within CA265_RS17335 at 4330.541 kb on + strand, within CA265_RS17335 at 4330.542 kb on - strand, within CA265_RS17335 at 4330.565 kb on + strand, within CA265_RS17335 at 4330.566 kb on - strand, within CA265_RS17335 at 4330.652 kb on + strand, within CA265_RS17335 at 4330.705 kb on + strand at 4330.706 kb on - strand at 4330.798 kb on + strand, within CA265_RS17340 at 4330.799 kb on - strand, within CA265_RS17340 at 4330.799 kb on - strand, within CA265_RS17340 at 4330.826 kb on + strand, within CA265_RS17340 at 4330.829 kb on - strand, within CA265_RS17340 at 4330.923 kb on + strand, within CA265_RS17340 at 4330.931 kb on - strand, within CA265_RS17340 at 4331.038 kb on - strand, within CA265_RS17340
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.02 mg/ml remove 4,328,904 - -0.4 4,328,909 + -0.2 4,328,909 + -0.7 4,328,909 + -1.8 4,328,910 - -0.2 4,328,910 - -1.4 4,328,910 - +0.4 4,328,980 + -0.7 4,329,049 - +1.6 4,329,053 - -0.2 4,329,081 - -0.4 4,329,099 + CA265_RS17325 0.12 -1.1 4,329,169 + CA265_RS17325 0.25 -0.5 4,329,171 + CA265_RS17325 0.25 -0.2 4,329,171 + CA265_RS17325 0.25 +0.8 4,329,172 - CA265_RS17325 0.25 -1.4 4,329,172 - CA265_RS17325 0.25 -1.3 4,329,175 - CA265_RS17325 0.26 -0.9 4,329,175 - CA265_RS17325 0.26 -0.4 4,329,177 + CA265_RS17325 0.26 +0.3 4,329,178 - CA265_RS17325 0.26 -1.1 4,329,178 - CA265_RS17325 0.26 -1.4 4,329,181 - CA265_RS17325 0.27 +0.6 4,329,290 + CA265_RS17325 0.45 -0.3 4,329,293 + CA265_RS17325 0.46 +1.0 4,329,293 + CA265_RS17325 0.46 +0.6 4,329,351 + CA265_RS17325 0.56 -0.3 4,329,352 - CA265_RS17325 0.56 -1.4 4,329,356 - CA265_RS17325 0.57 -1.4 4,329,369 - CA265_RS17325 0.59 -1.0 4,329,392 + CA265_RS17325 0.63 +0.0 4,329,491 + CA265_RS17325 0.80 +0.1 4,329,579 - -0.3 4,329,579 - -0.0 4,329,638 + -0.7 4,329,639 - +0.4 4,329,645 + -0.8 4,329,645 + -0.7 4,329,679 + -0.3 4,329,705 + -1.1 4,329,714 + -2.6 4,329,787 - -0.6 4,329,804 - -0.7 4,329,981 - CA265_RS17330 0.50 -0.1 4,329,993 - CA265_RS17330 0.53 -2.1 4,330,065 - CA265_RS17330 0.76 -0.4 4,330,074 + CA265_RS17330 0.79 +0.7 4,330,075 - CA265_RS17330 0.79 -1.1 4,330,140 + +0.7 4,330,151 - -0.1 4,330,170 + -1.1 4,330,170 + +0.2 4,330,170 + +0.1 4,330,171 - -1.0 4,330,171 - -0.0 4,330,172 + -0.1 4,330,173 - -1.3 4,330,206 - +0.1 4,330,224 + -0.9 4,330,229 - +1.7 4,330,267 - -0.0 4,330,302 - CA265_RS17335 0.14 +0.3 4,330,328 - CA265_RS17335 0.19 -0.2 4,330,338 - CA265_RS17335 0.21 +0.1 4,330,343 + CA265_RS17335 0.22 -0.5 4,330,344 - CA265_RS17335 0.22 -0.1 4,330,363 - CA265_RS17335 0.26 -0.6 4,330,371 + CA265_RS17335 0.27 -0.2 4,330,371 + CA265_RS17335 0.27 -1.9 4,330,418 - CA265_RS17335 0.36 +0.4 4,330,419 + CA265_RS17335 0.37 -0.4 4,330,419 + CA265_RS17335 0.37 -0.4 4,330,420 - CA265_RS17335 0.37 +0.5 4,330,541 + CA265_RS17335 0.60 +0.4 4,330,542 - CA265_RS17335 0.60 -0.6 4,330,565 + CA265_RS17335 0.65 +1.1 4,330,566 - CA265_RS17335 0.65 +0.3 4,330,652 + CA265_RS17335 0.82 -0.5 4,330,705 + -1.1 4,330,706 - +0.0 4,330,798 + CA265_RS17340 0.12 -0.2 4,330,799 - CA265_RS17340 0.13 +0.1 4,330,799 - CA265_RS17340 0.13 -0.6 4,330,826 + CA265_RS17340 0.17 -0.4 4,330,829 - CA265_RS17340 0.18 -1.1 4,330,923 + CA265_RS17340 0.35 -0.5 4,330,931 - CA265_RS17340 0.36 -0.1 4,331,038 - CA265_RS17340 0.56 +0.9
Or see this region's nucleotide sequence