Experiment: R2A with A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS17280 and CA265_RS17285 are separated by 468 nucleotides CA265_RS17285 and CA265_RS17290 are separated by 108 nucleotides CA265_RS17290 and CA265_RS17295 are separated by 125 nucleotides CA265_RS17295 and CA265_RS17300 are separated by 104 nucleotides
CA265_RS17280: CA265_RS17280 - hypothetical protein, at 4,321,024 to 4,323,105
_RS17280
CA265_RS17285: CA265_RS17285 - hypothetical protein, at 4,323,574 to 4,323,765
_RS17285
CA265_RS17290: CA265_RS17290 - tRNA-Lys, at 4,323,874 to 4,323,949
_RS17290
CA265_RS17295: CA265_RS17295 - hypothetical protein, at 4,324,075 to 4,324,371
_RS17295
CA265_RS17300: CA265_RS17300 - hypothetical protein, at 4,324,476 to 4,324,733
_RS17300
Position (kb)
4323
4324 Strain fitness (log2 ratio)
-1
0
1 at 4322.994 kb on - strand at 4323.024 kb on + strand at 4323.107 kb on + strand at 4323.108 kb on - strand at 4323.108 kb on - strand at 4323.109 kb on + strand at 4323.109 kb on + strand at 4323.110 kb on - strand at 4323.159 kb on + strand at 4323.159 kb on + strand at 4323.159 kb on + strand at 4323.159 kb on + strand at 4323.239 kb on + strand at 4323.240 kb on - strand at 4323.243 kb on - strand at 4323.249 kb on + strand at 4323.270 kb on + strand at 4323.270 kb on + strand at 4323.364 kb on + strand at 4323.439 kb on + strand at 4323.663 kb on + strand, within CA265_RS17285 at 4323.664 kb on - strand, within CA265_RS17285 at 4323.670 kb on + strand, within CA265_RS17285 at 4323.721 kb on - strand, within CA265_RS17285 at 4323.829 kb on - strand at 4323.829 kb on - strand at 4323.868 kb on - strand at 4323.913 kb on + strand, within CA265_RS17290 at 4323.942 kb on - strand at 4323.965 kb on - strand at 4323.986 kb on - strand at 4324.006 kb on + strand at 4324.024 kb on - strand at 4324.025 kb on + strand at 4324.084 kb on + strand at 4324.089 kb on + strand at 4324.100 kb on + strand at 4324.134 kb on + strand, within CA265_RS17295 at 4324.146 kb on - strand, within CA265_RS17295 at 4324.146 kb on - strand, within CA265_RS17295 at 4324.169 kb on + strand, within CA265_RS17295 at 4324.192 kb on + strand at 4324.194 kb on + strand, within CA265_RS17295 at 4324.194 kb on + strand, within CA265_RS17295 at 4324.195 kb on - strand, within CA265_RS17295 at 4324.195 kb on - strand, within CA265_RS17295 at 4324.230 kb on + strand, within CA265_RS17295 at 4324.238 kb on + strand, within CA265_RS17295 at 4324.256 kb on + strand, within CA265_RS17295 at 4324.328 kb on - strand, within CA265_RS17295 at 4324.382 kb on + strand at 4324.416 kb on - strand at 4324.419 kb on - strand at 4324.475 kb on + strand at 4324.557 kb on - strand, within CA265_RS17300 at 4324.557 kb on - strand, within CA265_RS17300 at 4324.561 kb on - strand, within CA265_RS17300 at 4324.562 kb on + strand, within CA265_RS17300 at 4324.566 kb on - strand, within CA265_RS17300 at 4324.566 kb on - strand, within CA265_RS17300 at 4324.593 kb on + strand, within CA265_RS17300 at 4324.593 kb on + strand, within CA265_RS17300 at 4324.593 kb on + strand, within CA265_RS17300 at 4324.611 kb on + strand, within CA265_RS17300 at 4324.661 kb on + strand, within CA265_RS17300 at 4324.661 kb on + strand, within CA265_RS17300 at 4324.661 kb on + strand, within CA265_RS17300 at 4324.668 kb on - strand, within CA265_RS17300 at 4324.668 kb on - strand, within CA265_RS17300 at 4324.668 kb on - strand, within CA265_RS17300 at 4324.690 kb on + strand, within CA265_RS17300 at 4324.741 kb on - strand at 4324.745 kb on - strand at 4324.746 kb on + strand at 4324.753 kb on - strand at 4324.757 kb on - strand at 4324.839 kb on - strand at 4324.844 kb on - strand at 4324.844 kb on - strand at 4324.876 kb on + strand at 4324.877 kb on - strand at 4324.877 kb on - strand at 4324.914 kb on + strand at 4324.918 kb on + strand at 4324.918 kb on + strand at 4324.919 kb on - strand at 4324.919 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.02 mg/ml remove 4,322,994 - -0.2 4,323,024 + +0.5 4,323,107 + -0.6 4,323,108 - +0.9 4,323,108 - -0.1 4,323,109 + -0.9 4,323,109 + -1.0 4,323,110 - -0.2 4,323,159 + -0.4 4,323,159 + -0.4 4,323,159 + -0.6 4,323,159 + +1.5 4,323,239 + -0.0 4,323,240 - -0.9 4,323,243 - -1.2 4,323,249 + +0.8 4,323,270 + -0.1 4,323,270 + -0.9 4,323,364 + -0.0 4,323,439 + -0.4 4,323,663 + CA265_RS17285 0.46 -0.1 4,323,664 - CA265_RS17285 0.47 +0.2 4,323,670 + CA265_RS17285 0.50 -0.3 4,323,721 - CA265_RS17285 0.77 +0.7 4,323,829 - -1.0 4,323,829 - -0.0 4,323,868 - +0.0 4,323,913 + CA265_RS17290 0.51 -0.8 4,323,942 - -0.7 4,323,965 - -1.7 4,323,986 - -0.7 4,324,006 + -0.5 4,324,024 - +0.1 4,324,025 + +0.1 4,324,084 + -0.1 4,324,089 + +0.5 4,324,100 + +0.2 4,324,134 + CA265_RS17295 0.20 +0.2 4,324,146 - CA265_RS17295 0.24 -0.9 4,324,146 - CA265_RS17295 0.24 -1.1 4,324,169 + CA265_RS17295 0.32 -0.4 4,324,192 + +0.7 4,324,194 + CA265_RS17295 0.40 +0.7 4,324,194 + CA265_RS17295 0.40 +0.0 4,324,195 - CA265_RS17295 0.40 -0.4 4,324,195 - CA265_RS17295 0.40 -0.9 4,324,230 + CA265_RS17295 0.52 -0.6 4,324,238 + CA265_RS17295 0.55 -0.5 4,324,256 + CA265_RS17295 0.61 +0.0 4,324,328 - CA265_RS17295 0.85 +0.8 4,324,382 + +0.4 4,324,416 - -0.6 4,324,419 - +0.5 4,324,475 + -0.0 4,324,557 - CA265_RS17300 0.31 +0.6 4,324,557 - CA265_RS17300 0.31 +0.2 4,324,561 - CA265_RS17300 0.33 -1.4 4,324,562 + CA265_RS17300 0.33 -0.4 4,324,566 - CA265_RS17300 0.35 +0.1 4,324,566 - CA265_RS17300 0.35 -1.1 4,324,593 + CA265_RS17300 0.45 -0.1 4,324,593 + CA265_RS17300 0.45 +0.4 4,324,593 + CA265_RS17300 0.45 -1.5 4,324,611 + CA265_RS17300 0.52 -0.1 4,324,661 + CA265_RS17300 0.72 +0.0 4,324,661 + CA265_RS17300 0.72 +0.7 4,324,661 + CA265_RS17300 0.72 -0.5 4,324,668 - CA265_RS17300 0.74 -1.2 4,324,668 - CA265_RS17300 0.74 -0.1 4,324,668 - CA265_RS17300 0.74 -0.5 4,324,690 + CA265_RS17300 0.83 +0.6 4,324,741 - -1.2 4,324,745 - +0.5 4,324,746 + +0.7 4,324,753 - -0.2 4,324,757 - -0.3 4,324,839 - +0.0 4,324,844 - -0.1 4,324,844 - +0.1 4,324,876 + -0.6 4,324,877 - -0.3 4,324,877 - +0.3 4,324,914 + +1.0 4,324,918 + +0.1 4,324,918 + -0.2 4,324,919 - -1.3 4,324,919 - +0.9
Or see this region's nucleotide sequence