Experiment: R2A with A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS15610 and CA265_RS15615 are separated by 110 nucleotides CA265_RS15615 and CA265_RS15620 are separated by 257 nucleotides
CA265_RS15610: CA265_RS15610 - bacillithiol biosynthesis cysteine-adding enzyme BshC, at 3,928,513 to 3,930,099
_RS15610
CA265_RS15615: CA265_RS15615 - malate dehydrogenase, at 3,930,210 to 3,931,295
_RS15615
CA265_RS15620: CA265_RS15620 - beta-1,3-glucosyltransferase, at 3,931,553 to 3,934,225
_RS15620
Position (kb)
3930
3931
3932 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3930.097 kb on + strand at 3930.100 kb on + strand at 3930.100 kb on + strand at 3930.166 kb on - strand at 3930.171 kb on + strand at 3930.171 kb on + strand at 3930.172 kb on - strand at 3930.175 kb on + strand at 3930.175 kb on + strand at 3930.176 kb on - strand at 3930.202 kb on + strand at 3930.207 kb on - strand at 3930.417 kb on - strand, within CA265_RS15615 at 3930.455 kb on - strand, within CA265_RS15615 at 3930.455 kb on - strand, within CA265_RS15615 at 3930.484 kb on + strand, within CA265_RS15615 at 3930.592 kb on + strand, within CA265_RS15615 at 3930.624 kb on - strand, within CA265_RS15615 at 3930.660 kb on + strand, within CA265_RS15615 at 3930.731 kb on - strand, within CA265_RS15615 at 3930.736 kb on + strand, within CA265_RS15615 at 3930.915 kb on - strand, within CA265_RS15615 at 3930.944 kb on + strand, within CA265_RS15615 at 3930.945 kb on - strand, within CA265_RS15615 at 3930.975 kb on + strand, within CA265_RS15615 at 3930.976 kb on - strand, within CA265_RS15615 at 3931.131 kb on - strand, within CA265_RS15615 at 3931.131 kb on - strand, within CA265_RS15615 at 3931.165 kb on + strand, within CA265_RS15615 at 3931.293 kb on + strand at 3931.314 kb on + strand at 3931.315 kb on - strand at 3931.334 kb on - strand at 3931.334 kb on - strand at 3931.334 kb on - strand at 3931.347 kb on + strand at 3931.561 kb on + strand at 3931.580 kb on + strand at 3931.584 kb on + strand at 3931.585 kb on - strand at 3931.654 kb on + strand at 3931.670 kb on + strand at 3931.719 kb on - strand at 3931.849 kb on + strand, within CA265_RS15620 at 3931.915 kb on - strand, within CA265_RS15620 at 3931.915 kb on - strand, within CA265_RS15620 at 3931.927 kb on - strand, within CA265_RS15620 at 3931.963 kb on + strand, within CA265_RS15620 at 3931.965 kb on + strand, within CA265_RS15620 at 3932.072 kb on + strand, within CA265_RS15620 at 3932.076 kb on - strand, within CA265_RS15620 at 3932.159 kb on - strand, within CA265_RS15620 at 3932.189 kb on + strand, within CA265_RS15620 at 3932.226 kb on - strand, within CA265_RS15620 at 3932.252 kb on + strand, within CA265_RS15620 at 3932.285 kb on + strand, within CA265_RS15620
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.02 mg/ml remove 3,930,097 + +0.1 3,930,100 + -0.1 3,930,100 + +0.5 3,930,166 - -0.4 3,930,171 + -0.4 3,930,171 + -0.2 3,930,172 - -0.4 3,930,175 + +0.4 3,930,175 + -0.6 3,930,176 - +0.0 3,930,202 + -0.7 3,930,207 - -0.5 3,930,417 - CA265_RS15615 0.19 -0.8 3,930,455 - CA265_RS15615 0.23 -0.4 3,930,455 - CA265_RS15615 0.23 -1.0 3,930,484 + CA265_RS15615 0.25 -3.1 3,930,592 + CA265_RS15615 0.35 -1.4 3,930,624 - CA265_RS15615 0.38 -0.1 3,930,660 + CA265_RS15615 0.41 -2.4 3,930,731 - CA265_RS15615 0.48 -1.2 3,930,736 + CA265_RS15615 0.48 +0.4 3,930,915 - CA265_RS15615 0.65 -0.7 3,930,944 + CA265_RS15615 0.68 -3.0 3,930,945 - CA265_RS15615 0.68 -1.7 3,930,975 + CA265_RS15615 0.70 -0.9 3,930,976 - CA265_RS15615 0.71 -1.0 3,931,131 - CA265_RS15615 0.85 -1.2 3,931,131 - CA265_RS15615 0.85 -1.0 3,931,165 + CA265_RS15615 0.88 -1.6 3,931,293 + -0.2 3,931,314 + +1.0 3,931,315 - -0.7 3,931,334 - -0.1 3,931,334 - -0.2 3,931,334 - +0.6 3,931,347 + -0.1 3,931,561 + +0.9 3,931,580 + +0.1 3,931,584 + +1.5 3,931,585 - -1.3 3,931,654 + +0.2 3,931,670 + -0.4 3,931,719 - +0.0 3,931,849 + CA265_RS15620 0.11 +1.1 3,931,915 - CA265_RS15620 0.14 -0.7 3,931,915 - CA265_RS15620 0.14 -0.1 3,931,927 - CA265_RS15620 0.14 -1.0 3,931,963 + CA265_RS15620 0.15 +2.0 3,931,965 + CA265_RS15620 0.15 -0.8 3,932,072 + CA265_RS15620 0.19 +0.3 3,932,076 - CA265_RS15620 0.20 -0.1 3,932,159 - CA265_RS15620 0.23 +0.6 3,932,189 + CA265_RS15620 0.24 -0.1 3,932,226 - CA265_RS15620 0.25 -0.7 3,932,252 + CA265_RS15620 0.26 +1.1 3,932,285 + CA265_RS15620 0.27 -0.1
Or see this region's nucleotide sequence