Experiment: R2A with A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS14995 and CA265_RS15000 are separated by 115 nucleotides CA265_RS15000 and CA265_RS15005 are separated by 97 nucleotides
CA265_RS14995: CA265_RS14995 - sodium-translocating pyrophosphatase, at 3,778,646 to 3,780,892
_RS14995
CA265_RS15000: CA265_RS15000 - amino acid permease, at 3,781,008 to 3,782,483
_RS15000
CA265_RS15005: CA265_RS15005 - porin, at 3,782,581 to 3,783,912
_RS15005
Position (kb)
3781
3782
3783 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3780.064 kb on - strand, within CA265_RS14995 at 3780.148 kb on - strand, within CA265_RS14995 at 3780.162 kb on + strand, within CA265_RS14995 at 3780.162 kb on + strand, within CA265_RS14995 at 3780.241 kb on - strand, within CA265_RS14995 at 3780.247 kb on + strand, within CA265_RS14995 at 3780.248 kb on - strand, within CA265_RS14995 at 3780.352 kb on + strand, within CA265_RS14995 at 3780.360 kb on - strand, within CA265_RS14995 at 3780.361 kb on + strand, within CA265_RS14995 at 3780.361 kb on + strand, within CA265_RS14995 at 3780.362 kb on - strand, within CA265_RS14995 at 3780.378 kb on - strand, within CA265_RS14995 at 3780.451 kb on + strand, within CA265_RS14995 at 3780.501 kb on + strand, within CA265_RS14995 at 3780.502 kb on - strand, within CA265_RS14995 at 3780.502 kb on - strand, within CA265_RS14995 at 3780.528 kb on + strand, within CA265_RS14995 at 3780.529 kb on - strand, within CA265_RS14995 at 3780.724 kb on - strand at 3780.745 kb on + strand at 3780.755 kb on + strand at 3780.756 kb on - strand at 3780.756 kb on - strand at 3780.838 kb on - strand at 3780.844 kb on + strand at 3780.909 kb on - strand at 3780.909 kb on - strand at 3780.909 kb on - strand at 3780.987 kb on - strand at 3781.004 kb on + strand at 3781.007 kb on + strand at 3781.040 kb on - strand at 3781.142 kb on - strand at 3781.216 kb on + strand, within CA265_RS15000 at 3781.217 kb on - strand, within CA265_RS15000 at 3781.237 kb on + strand, within CA265_RS15000 at 3781.238 kb on - strand, within CA265_RS15000 at 3781.285 kb on - strand, within CA265_RS15000 at 3781.287 kb on - strand, within CA265_RS15000 at 3781.287 kb on - strand, within CA265_RS15000 at 3781.287 kb on - strand, within CA265_RS15000 at 3781.290 kb on + strand, within CA265_RS15000 at 3781.292 kb on + strand, within CA265_RS15000 at 3781.296 kb on + strand, within CA265_RS15000 at 3781.316 kb on - strand, within CA265_RS15000 at 3781.354 kb on - strand, within CA265_RS15000 at 3781.370 kb on + strand, within CA265_RS15000 at 3781.375 kb on + strand, within CA265_RS15000 at 3781.376 kb on - strand, within CA265_RS15000 at 3781.376 kb on - strand, within CA265_RS15000 at 3781.408 kb on + strand, within CA265_RS15000 at 3781.409 kb on - strand, within CA265_RS15000 at 3781.423 kb on + strand, within CA265_RS15000 at 3781.424 kb on - strand, within CA265_RS15000 at 3781.427 kb on + strand, within CA265_RS15000 at 3781.427 kb on + strand, within CA265_RS15000 at 3781.428 kb on - strand, within CA265_RS15000 at 3781.490 kb on - strand, within CA265_RS15000 at 3781.618 kb on + strand, within CA265_RS15000 at 3781.689 kb on + strand, within CA265_RS15000 at 3781.689 kb on + strand, within CA265_RS15000 at 3781.812 kb on - strand, within CA265_RS15000 at 3781.866 kb on - strand, within CA265_RS15000 at 3781.873 kb on + strand, within CA265_RS15000 at 3781.873 kb on + strand, within CA265_RS15000 at 3781.874 kb on - strand, within CA265_RS15000 at 3781.875 kb on + strand, within CA265_RS15000 at 3781.876 kb on - strand, within CA265_RS15000 at 3781.877 kb on + strand, within CA265_RS15000 at 3781.951 kb on + strand, within CA265_RS15000 at 3781.998 kb on - strand, within CA265_RS15000 at 3782.003 kb on - strand, within CA265_RS15000 at 3782.039 kb on - strand, within CA265_RS15000 at 3782.176 kb on + strand, within CA265_RS15000 at 3782.234 kb on + strand, within CA265_RS15000 at 3782.235 kb on - strand, within CA265_RS15000 at 3782.249 kb on + strand, within CA265_RS15000 at 3782.270 kb on - strand, within CA265_RS15000 at 3782.365 kb on + strand at 3782.451 kb on - strand at 3782.538 kb on + strand at 3782.542 kb on + strand at 3782.614 kb on + strand at 3782.615 kb on - strand at 3782.659 kb on + strand at 3782.748 kb on + strand, within CA265_RS15005 at 3782.759 kb on + strand, within CA265_RS15005 at 3782.759 kb on + strand, within CA265_RS15005 at 3782.806 kb on + strand, within CA265_RS15005 at 3782.853 kb on - strand, within CA265_RS15005 at 3782.867 kb on + strand, within CA265_RS15005 at 3782.878 kb on + strand, within CA265_RS15005 at 3782.878 kb on + strand, within CA265_RS15005 at 3782.878 kb on + strand, within CA265_RS15005 at 3782.878 kb on + strand, within CA265_RS15005 at 3782.879 kb on - strand, within CA265_RS15005 at 3782.879 kb on - strand, within CA265_RS15005 at 3782.880 kb on + strand, within CA265_RS15005 at 3782.910 kb on + strand, within CA265_RS15005 at 3782.914 kb on + strand, within CA265_RS15005 at 3782.977 kb on + strand, within CA265_RS15005 at 3782.978 kb on - strand, within CA265_RS15005 at 3782.981 kb on - strand, within CA265_RS15005 at 3782.988 kb on + strand, within CA265_RS15005 at 3782.988 kb on + strand, within CA265_RS15005 at 3783.132 kb on + strand, within CA265_RS15005 at 3783.133 kb on - strand, within CA265_RS15005 at 3783.139 kb on - strand, within CA265_RS15005 at 3783.199 kb on + strand, within CA265_RS15005 at 3783.244 kb on - strand, within CA265_RS15005 at 3783.299 kb on + strand, within CA265_RS15005 at 3783.300 kb on - strand, within CA265_RS15005 at 3783.315 kb on + strand, within CA265_RS15005 at 3783.321 kb on - strand, within CA265_RS15005 at 3783.356 kb on - strand, within CA265_RS15005 at 3783.440 kb on - strand, within CA265_RS15005 at 3783.440 kb on - strand, within CA265_RS15005 at 3783.442 kb on + strand, within CA265_RS15005 at 3783.443 kb on - strand, within CA265_RS15005 at 3783.466 kb on - strand, within CA265_RS15005
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.02 mg/ml remove 3,780,064 - CA265_RS14995 0.63 -0.2 3,780,148 - CA265_RS14995 0.67 +0.3 3,780,162 + CA265_RS14995 0.67 -1.1 3,780,162 + CA265_RS14995 0.67 -0.2 3,780,241 - CA265_RS14995 0.71 -0.3 3,780,247 + CA265_RS14995 0.71 +0.5 3,780,248 - CA265_RS14995 0.71 -1.0 3,780,352 + CA265_RS14995 0.76 +0.2 3,780,360 - CA265_RS14995 0.76 +0.4 3,780,361 + CA265_RS14995 0.76 -0.5 3,780,361 + CA265_RS14995 0.76 -0.2 3,780,362 - CA265_RS14995 0.76 -0.3 3,780,378 - CA265_RS14995 0.77 +0.6 3,780,451 + CA265_RS14995 0.80 +0.3 3,780,501 + CA265_RS14995 0.83 -0.8 3,780,502 - CA265_RS14995 0.83 +0.0 3,780,502 - CA265_RS14995 0.83 -0.2 3,780,528 + CA265_RS14995 0.84 -0.3 3,780,529 - CA265_RS14995 0.84 -0.8 3,780,724 - -0.1 3,780,745 + -0.1 3,780,755 + -0.6 3,780,756 - +0.1 3,780,756 - +0.5 3,780,838 - +0.7 3,780,844 + +0.2 3,780,909 - -0.8 3,780,909 - -0.1 3,780,909 - -0.2 3,780,987 - -0.6 3,781,004 + -1.5 3,781,007 + -3.2 3,781,040 - -0.9 3,781,142 - -0.7 3,781,216 + CA265_RS15000 0.14 -0.3 3,781,217 - CA265_RS15000 0.14 -2.5 3,781,237 + CA265_RS15000 0.16 -1.4 3,781,238 - CA265_RS15000 0.16 -0.6 3,781,285 - CA265_RS15000 0.19 -2.8 3,781,287 - CA265_RS15000 0.19 -0.9 3,781,287 - CA265_RS15000 0.19 -1.2 3,781,287 - CA265_RS15000 0.19 -1.4 3,781,290 + CA265_RS15000 0.19 -1.2 3,781,292 + CA265_RS15000 0.19 -0.1 3,781,296 + CA265_RS15000 0.20 +0.3 3,781,316 - CA265_RS15000 0.21 -1.3 3,781,354 - CA265_RS15000 0.23 -1.6 3,781,370 + CA265_RS15000 0.25 -1.1 3,781,375 + CA265_RS15000 0.25 -0.5 3,781,376 - CA265_RS15000 0.25 -1.0 3,781,376 - CA265_RS15000 0.25 -1.4 3,781,408 + CA265_RS15000 0.27 -2.7 3,781,409 - CA265_RS15000 0.27 +0.5 3,781,423 + CA265_RS15000 0.28 -1.5 3,781,424 - CA265_RS15000 0.28 +0.2 3,781,427 + CA265_RS15000 0.28 -2.1 3,781,427 + CA265_RS15000 0.28 -0.8 3,781,428 - CA265_RS15000 0.28 -1.0 3,781,490 - CA265_RS15000 0.33 -0.8 3,781,618 + CA265_RS15000 0.41 -1.3 3,781,689 + CA265_RS15000 0.46 -1.4 3,781,689 + CA265_RS15000 0.46 -1.5 3,781,812 - CA265_RS15000 0.54 -0.8 3,781,866 - CA265_RS15000 0.58 -1.6 3,781,873 + CA265_RS15000 0.59 -0.2 3,781,873 + CA265_RS15000 0.59 -1.1 3,781,874 - CA265_RS15000 0.59 -1.1 3,781,875 + CA265_RS15000 0.59 +0.1 3,781,876 - CA265_RS15000 0.59 +0.3 3,781,877 + CA265_RS15000 0.59 -1.9 3,781,951 + CA265_RS15000 0.64 -0.8 3,781,998 - CA265_RS15000 0.67 -0.5 3,782,003 - CA265_RS15000 0.67 -0.6 3,782,039 - CA265_RS15000 0.70 -0.2 3,782,176 + CA265_RS15000 0.79 -1.8 3,782,234 + CA265_RS15000 0.83 -0.1 3,782,235 - CA265_RS15000 0.83 -1.1 3,782,249 + CA265_RS15000 0.84 +1.5 3,782,270 - CA265_RS15000 0.86 +0.8 3,782,365 + -0.8 3,782,451 - -0.8 3,782,538 + +0.5 3,782,542 + -0.0 3,782,614 + +0.2 3,782,615 - -0.3 3,782,659 + -0.3 3,782,748 + CA265_RS15005 0.13 -2.1 3,782,759 + CA265_RS15005 0.13 -0.5 3,782,759 + CA265_RS15005 0.13 +1.3 3,782,806 + CA265_RS15005 0.17 +0.1 3,782,853 - CA265_RS15005 0.20 +0.5 3,782,867 + CA265_RS15005 0.21 -0.3 3,782,878 + CA265_RS15005 0.22 -0.3 3,782,878 + CA265_RS15005 0.22 +0.2 3,782,878 + CA265_RS15005 0.22 -0.1 3,782,878 + CA265_RS15005 0.22 +1.9 3,782,879 - CA265_RS15005 0.22 +1.2 3,782,879 - CA265_RS15005 0.22 -0.6 3,782,880 + CA265_RS15005 0.22 -0.6 3,782,910 + CA265_RS15005 0.25 -0.1 3,782,914 + CA265_RS15005 0.25 -0.1 3,782,977 + CA265_RS15005 0.30 +0.2 3,782,978 - CA265_RS15005 0.30 -2.3 3,782,981 - CA265_RS15005 0.30 -0.1 3,782,988 + CA265_RS15005 0.31 +0.3 3,782,988 + CA265_RS15005 0.31 -0.3 3,783,132 + CA265_RS15005 0.41 +0.2 3,783,133 - CA265_RS15005 0.41 +0.3 3,783,139 - CA265_RS15005 0.42 +0.1 3,783,199 + CA265_RS15005 0.46 -0.2 3,783,244 - CA265_RS15005 0.50 +0.2 3,783,299 + CA265_RS15005 0.54 +1.1 3,783,300 - CA265_RS15005 0.54 -0.3 3,783,315 + CA265_RS15005 0.55 +0.5 3,783,321 - CA265_RS15005 0.56 +1.2 3,783,356 - CA265_RS15005 0.58 +0.5 3,783,440 - CA265_RS15005 0.64 +0.2 3,783,440 - CA265_RS15005 0.64 -0.2 3,783,442 + CA265_RS15005 0.65 -0.4 3,783,443 - CA265_RS15005 0.65 +0.0 3,783,466 - CA265_RS15005 0.66 -0.2
Or see this region's nucleotide sequence