Experiment: R2A with A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS14520 and CA265_RS14525 are separated by 99 nucleotides CA265_RS14525 and CA265_RS14530 are separated by 49 nucleotides CA265_RS14530 and CA265_RS14535 are separated by 138 nucleotides CA265_RS14535 and CA265_RS14540 are separated by 173 nucleotides
CA265_RS14520: CA265_RS14520 - peptidase M48, at 3,659,233 to 3,660,039
_RS14520
CA265_RS14525: CA265_RS14525 - acyl-phosphate glycerol 3-phosphate acyltransferase, at 3,660,139 to 3,660,786
_RS14525
CA265_RS14530: CA265_RS14530 - nicotinate-nucleotide diphosphorylase (carboxylating), at 3,660,836 to 3,661,678
_RS14530
CA265_RS14535: CA265_RS14535 - DUF4783 domain-containing protein, at 3,661,817 to 3,662,215
_RS14535
CA265_RS14540: CA265_RS14540 - phosphoglycerate mutase (2,3-diphosphoglycerate-independent), at 3,662,389 to 3,663,912
_RS14540
Position (kb)
3660
3661
3662 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3659.836 kb on - strand, within CA265_RS14520 at 3659.836 kb on - strand, within CA265_RS14520 at 3659.836 kb on - strand, within CA265_RS14520 at 3659.867 kb on - strand, within CA265_RS14520 at 3659.958 kb on + strand, within CA265_RS14520 at 3660.052 kb on - strand at 3660.066 kb on + strand at 3660.098 kb on + strand at 3660.098 kb on + strand at 3660.105 kb on - strand at 3660.111 kb on - strand at 3660.111 kb on - strand at 3660.838 kb on - strand at 3660.905 kb on + strand at 3660.922 kb on - strand, within CA265_RS14530 at 3660.934 kb on - strand, within CA265_RS14530 at 3661.031 kb on - strand at 3661.345 kb on + strand, within CA265_RS14530 at 3661.414 kb on + strand, within CA265_RS14530 at 3661.475 kb on + strand, within CA265_RS14530 at 3661.691 kb on - strand at 3661.718 kb on - strand at 3661.730 kb on - strand at 3661.753 kb on - strand at 3661.757 kb on + strand at 3661.757 kb on + strand at 3661.759 kb on + strand at 3661.913 kb on - strand, within CA265_RS14535 at 3662.170 kb on - strand, within CA265_RS14535 at 3662.171 kb on + strand, within CA265_RS14535 at 3662.171 kb on + strand, within CA265_RS14535 at 3662.186 kb on - strand at 3662.202 kb on - strand at 3662.239 kb on - strand at 3662.245 kb on - strand at 3662.307 kb on + strand at 3662.318 kb on + strand at 3662.318 kb on + strand at 3662.344 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.02 mg/ml remove 3,659,836 - CA265_RS14520 0.75 -0.1 3,659,836 - CA265_RS14520 0.75 +0.8 3,659,836 - CA265_RS14520 0.75 -0.6 3,659,867 - CA265_RS14520 0.79 +0.6 3,659,958 + CA265_RS14520 0.90 -0.8 3,660,052 - -0.6 3,660,066 + +0.3 3,660,098 + -0.3 3,660,098 + -0.4 3,660,105 - -1.5 3,660,111 - +0.2 3,660,111 - +0.9 3,660,838 - -0.3 3,660,905 + -1.5 3,660,922 - CA265_RS14530 0.10 -0.8 3,660,934 - CA265_RS14530 0.12 -1.1 3,661,031 - +0.9 3,661,345 + CA265_RS14530 0.60 +0.3 3,661,414 + CA265_RS14530 0.69 -3.0 3,661,475 + CA265_RS14530 0.76 +1.0 3,661,691 - +0.2 3,661,718 - -0.7 3,661,730 - +0.3 3,661,753 - -0.0 3,661,757 + +0.2 3,661,757 + -0.3 3,661,759 + +1.5 3,661,913 - CA265_RS14535 0.24 -1.0 3,662,170 - CA265_RS14535 0.88 -2.1 3,662,171 + CA265_RS14535 0.89 -0.2 3,662,171 + CA265_RS14535 0.89 -0.7 3,662,186 - +0.2 3,662,202 - +0.4 3,662,239 - +0.4 3,662,245 - -0.1 3,662,307 + +0.4 3,662,318 + -0.0 3,662,318 + -1.5 3,662,344 + +0.8
Or see this region's nucleotide sequence