Experiment: R2A with A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS13970 and CA265_RS13975 overlap by 10 nucleotides CA265_RS13975 and CA265_RS13980 are separated by 59 nucleotides CA265_RS13980 and CA265_RS13985 are separated by 34 nucleotides CA265_RS13985 and CA265_RS13990 are separated by 130 nucleotides
CA265_RS13970: CA265_RS13970 - hypothetical protein, at 3,516,633 to 3,519,056
_RS13970
CA265_RS13975: CA265_RS13975 - RNA methyltransferase, at 3,519,047 to 3,519,793
_RS13975
CA265_RS13980: CA265_RS13980 - tRNA-Cys, at 3,519,853 to 3,519,926
_RS13980
CA265_RS13985: CA265_RS13985 - hypothetical protein, at 3,519,961 to 3,520,131
_RS13985
CA265_RS13990: CA265_RS13990 - hypothetical protein, at 3,520,262 to 3,521,254
_RS13990
Position (kb)
3519
3520
3521 Strain fitness (log2 ratio)
-2
-1
0
1 at 3518.963 kb on + strand at 3518.964 kb on - strand at 3518.964 kb on - strand at 3518.993 kb on + strand at 3518.997 kb on - strand at 3518.997 kb on - strand at 3519.001 kb on - strand at 3519.006 kb on + strand at 3519.007 kb on - strand at 3519.085 kb on - strand at 3519.179 kb on - strand, within CA265_RS13975 at 3519.203 kb on + strand, within CA265_RS13975 at 3519.274 kb on + strand, within CA265_RS13975 at 3519.275 kb on - strand, within CA265_RS13975 at 3519.275 kb on - strand, within CA265_RS13975 at 3519.277 kb on + strand, within CA265_RS13975 at 3519.314 kb on + strand, within CA265_RS13975 at 3519.314 kb on + strand, within CA265_RS13975 at 3519.314 kb on + strand, within CA265_RS13975 at 3519.315 kb on - strand, within CA265_RS13975 at 3519.375 kb on + strand, within CA265_RS13975 at 3519.406 kb on + strand, within CA265_RS13975 at 3519.469 kb on - strand, within CA265_RS13975 at 3519.553 kb on - strand, within CA265_RS13975 at 3519.937 kb on + strand at 3519.946 kb on + strand at 3520.044 kb on + strand, within CA265_RS13985 at 3520.044 kb on + strand, within CA265_RS13985 at 3520.044 kb on + strand, within CA265_RS13985 at 3520.116 kb on + strand at 3520.295 kb on + strand at 3520.319 kb on + strand at 3520.319 kb on + strand at 3520.319 kb on + strand at 3520.347 kb on + strand at 3520.451 kb on + strand, within CA265_RS13990 at 3520.452 kb on - strand, within CA265_RS13990 at 3520.475 kb on - strand, within CA265_RS13990 at 3520.597 kb on + strand, within CA265_RS13990 at 3520.598 kb on - strand, within CA265_RS13990 at 3520.601 kb on + strand, within CA265_RS13990 at 3520.624 kb on - strand, within CA265_RS13990 at 3520.695 kb on + strand, within CA265_RS13990 at 3520.699 kb on + strand, within CA265_RS13990 at 3520.788 kb on + strand, within CA265_RS13990 at 3520.894 kb on + strand, within CA265_RS13990 at 3520.934 kb on + strand, within CA265_RS13990 at 3520.941 kb on + strand, within CA265_RS13990 at 3520.941 kb on + strand, within CA265_RS13990 at 3520.951 kb on + strand, within CA265_RS13990 at 3521.007 kb on + strand, within CA265_RS13990 at 3521.008 kb on - strand, within CA265_RS13990 at 3521.008 kb on - strand, within CA265_RS13990 at 3521.018 kb on + strand, within CA265_RS13990 at 3521.018 kb on + strand, within CA265_RS13990 at 3521.019 kb on - strand, within CA265_RS13990 at 3521.082 kb on - strand, within CA265_RS13990
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.02 mg/ml remove 3,518,963 + +0.0 3,518,964 - +0.5 3,518,964 - -0.8 3,518,993 + +0.1 3,518,997 - -0.4 3,518,997 - -0.4 3,519,001 - -0.6 3,519,006 + -0.7 3,519,007 - -0.1 3,519,085 - -0.1 3,519,179 - CA265_RS13975 0.18 -0.0 3,519,203 + CA265_RS13975 0.21 -0.7 3,519,274 + CA265_RS13975 0.30 -0.8 3,519,275 - CA265_RS13975 0.31 -1.2 3,519,275 - CA265_RS13975 0.31 -0.3 3,519,277 + CA265_RS13975 0.31 -0.4 3,519,314 + CA265_RS13975 0.36 -0.8 3,519,314 + CA265_RS13975 0.36 -1.1 3,519,314 + CA265_RS13975 0.36 -0.1 3,519,315 - CA265_RS13975 0.36 -0.2 3,519,375 + CA265_RS13975 0.44 -1.4 3,519,406 + CA265_RS13975 0.48 -0.1 3,519,469 - CA265_RS13975 0.56 +0.2 3,519,553 - CA265_RS13975 0.68 -0.1 3,519,937 + -0.8 3,519,946 + -1.3 3,520,044 + CA265_RS13985 0.49 -0.1 3,520,044 + CA265_RS13985 0.49 -1.8 3,520,044 + CA265_RS13985 0.49 -0.4 3,520,116 + -0.3 3,520,295 + -0.5 3,520,319 + +0.7 3,520,319 + -0.6 3,520,319 + +0.2 3,520,347 + -0.4 3,520,451 + CA265_RS13990 0.19 -0.3 3,520,452 - CA265_RS13990 0.19 +0.4 3,520,475 - CA265_RS13990 0.21 +0.4 3,520,597 + CA265_RS13990 0.34 -0.5 3,520,598 - CA265_RS13990 0.34 -0.1 3,520,601 + CA265_RS13990 0.34 +0.1 3,520,624 - CA265_RS13990 0.36 -0.2 3,520,695 + CA265_RS13990 0.44 +0.4 3,520,699 + CA265_RS13990 0.44 -0.2 3,520,788 + CA265_RS13990 0.53 -0.2 3,520,894 + CA265_RS13990 0.64 +0.8 3,520,934 + CA265_RS13990 0.68 -0.4 3,520,941 + CA265_RS13990 0.68 -0.0 3,520,941 + CA265_RS13990 0.68 +0.5 3,520,951 + CA265_RS13990 0.69 -0.1 3,521,007 + CA265_RS13990 0.75 +0.0 3,521,008 - CA265_RS13990 0.75 -0.7 3,521,008 - CA265_RS13990 0.75 -1.4 3,521,018 + CA265_RS13990 0.76 +0.1 3,521,018 + CA265_RS13990 0.76 +0.2 3,521,019 - CA265_RS13990 0.76 +1.0 3,521,082 - CA265_RS13990 0.83 -1.1
Or see this region's nucleotide sequence