Experiment: R2A with A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS13165 and CA265_RS13170 are separated by 80 nucleotides CA265_RS13170 and CA265_RS13175 are separated by 344 nucleotides CA265_RS13175 and CA265_RS13180 are separated by 830 nucleotides
CA265_RS13165: CA265_RS13165 - MBL fold metallo-hydrolase, at 3,278,157 to 3,279,056
_RS13165
CA265_RS13170: CA265_RS13170 - hypothetical protein, at 3,279,137 to 3,279,340
_RS13170
CA265_RS13175: CA265_RS13175 - hypothetical protein, at 3,279,685 to 3,279,912
_RS13175
CA265_RS13180: CA265_RS13180 - cysteine desulfurase, at 3,280,743 to 3,281,891
_RS13180
Position (kb)
3279
3280 Strain fitness (log2 ratio)
-1
0
1
2 at 3278.745 kb on - strand at 3278.777 kb on + strand, within CA265_RS13165 at 3278.780 kb on + strand, within CA265_RS13165 at 3278.801 kb on + strand, within CA265_RS13165 at 3278.802 kb on - strand, within CA265_RS13165 at 3278.802 kb on - strand, within CA265_RS13165 at 3278.862 kb on + strand, within CA265_RS13165 at 3278.922 kb on + strand, within CA265_RS13165 at 3278.924 kb on + strand, within CA265_RS13165 at 3278.924 kb on + strand, within CA265_RS13165 at 3278.928 kb on + strand, within CA265_RS13165 at 3278.928 kb on + strand, within CA265_RS13165 at 3278.929 kb on - strand, within CA265_RS13165 at 3278.929 kb on - strand, within CA265_RS13165 at 3278.929 kb on - strand, within CA265_RS13165 at 3278.954 kb on + strand, within CA265_RS13165 at 3279.067 kb on - strand at 3279.118 kb on + strand at 3279.123 kb on + strand at 3279.139 kb on - strand at 3279.212 kb on - strand, within CA265_RS13170 at 3279.317 kb on - strand, within CA265_RS13170 at 3279.341 kb on - strand at 3279.341 kb on - strand at 3279.359 kb on - strand at 3279.380 kb on + strand at 3279.396 kb on - strand at 3279.417 kb on - strand at 3279.464 kb on + strand at 3279.471 kb on + strand at 3279.471 kb on + strand at 3279.472 kb on - strand at 3279.576 kb on + strand at 3279.576 kb on + strand at 3279.576 kb on + strand at 3279.584 kb on + strand at 3279.667 kb on + strand at 3279.667 kb on + strand at 3279.668 kb on - strand at 3279.668 kb on - strand at 3279.686 kb on + strand at 3279.686 kb on + strand at 3279.795 kb on + strand, within CA265_RS13175 at 3279.896 kb on + strand at 3279.897 kb on - strand at 3279.916 kb on + strand at 3279.971 kb on - strand at 3280.005 kb on + strand at 3280.008 kb on - strand at 3280.008 kb on - strand at 3280.069 kb on + strand at 3280.082 kb on + strand at 3280.083 kb on - strand at 3280.195 kb on - strand at 3280.198 kb on - strand at 3280.276 kb on - strand at 3280.276 kb on - strand at 3280.300 kb on - strand at 3280.331 kb on - strand at 3280.564 kb on - strand at 3280.595 kb on + strand at 3280.639 kb on + strand at 3280.740 kb on - strand at 3280.750 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.02 mg/ml remove 3,278,745 - +2.0 3,278,777 + CA265_RS13165 0.69 -0.5 3,278,780 + CA265_RS13165 0.69 -0.0 3,278,801 + CA265_RS13165 0.72 -0.5 3,278,802 - CA265_RS13165 0.72 -0.7 3,278,802 - CA265_RS13165 0.72 -0.6 3,278,862 + CA265_RS13165 0.78 -0.4 3,278,922 + CA265_RS13165 0.85 +0.1 3,278,924 + CA265_RS13165 0.85 +0.4 3,278,924 + CA265_RS13165 0.85 -0.4 3,278,928 + CA265_RS13165 0.86 -0.5 3,278,928 + CA265_RS13165 0.86 +0.3 3,278,929 - CA265_RS13165 0.86 -0.6 3,278,929 - CA265_RS13165 0.86 -0.7 3,278,929 - CA265_RS13165 0.86 -0.4 3,278,954 + CA265_RS13165 0.89 -0.6 3,279,067 - -0.3 3,279,118 + -0.6 3,279,123 + +0.2 3,279,139 - +0.5 3,279,212 - CA265_RS13170 0.37 +0.4 3,279,317 - CA265_RS13170 0.88 -0.2 3,279,341 - +0.7 3,279,341 - +0.5 3,279,359 - -0.6 3,279,380 + -0.7 3,279,396 - -0.7 3,279,417 - -0.7 3,279,464 + +0.4 3,279,471 + +0.1 3,279,471 + -0.9 3,279,472 - +0.1 3,279,576 + -0.7 3,279,576 + -0.2 3,279,576 + -0.5 3,279,584 + -0.4 3,279,667 + -0.1 3,279,667 + +0.9 3,279,668 - +0.1 3,279,668 - -0.3 3,279,686 + +0.6 3,279,686 + +0.4 3,279,795 + CA265_RS13175 0.48 -1.4 3,279,896 + -0.1 3,279,897 - -1.2 3,279,916 + -1.3 3,279,971 - +2.1 3,280,005 + -0.7 3,280,008 - +0.3 3,280,008 - -0.1 3,280,069 + -0.4 3,280,082 + -1.6 3,280,083 - -0.5 3,280,195 - +0.1 3,280,198 - -0.1 3,280,276 - +0.3 3,280,276 - +0.3 3,280,300 - -0.0 3,280,331 - +0.7 3,280,564 - -1.1 3,280,595 + +0.7 3,280,639 + -0.0 3,280,740 - -0.6 3,280,750 - -0.1
Or see this region's nucleotide sequence