Experiment: R2A with A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS11615 and CA265_RS11620 are separated by 1 nucleotides CA265_RS11620 and CA265_RS11625 are separated by 366 nucleotides
CA265_RS11615: CA265_RS11615 - 3-dehydroquinate synthase, at 2,880,144 to 2,881,202
_RS11615
CA265_RS11620: CA265_RS11620 - sterol desaturase, at 2,881,204 to 2,882,166
_RS11620
CA265_RS11625: CA265_RS11625 - hypothetical protein, at 2,882,533 to 2,883,150
_RS11625
Position (kb)
2881
2882
2883 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2881.307 kb on + strand, within CA265_RS11620 at 2881.311 kb on + strand, within CA265_RS11620 at 2881.311 kb on + strand, within CA265_RS11620 at 2881.314 kb on + strand, within CA265_RS11620 at 2881.335 kb on + strand, within CA265_RS11620 at 2881.335 kb on + strand, within CA265_RS11620 at 2881.336 kb on - strand, within CA265_RS11620 at 2881.336 kb on - strand, within CA265_RS11620 at 2881.336 kb on - strand, within CA265_RS11620 at 2881.371 kb on - strand, within CA265_RS11620 at 2881.411 kb on + strand, within CA265_RS11620 at 2881.470 kb on - strand, within CA265_RS11620 at 2881.513 kb on + strand, within CA265_RS11620 at 2881.513 kb on + strand, within CA265_RS11620 at 2881.514 kb on - strand, within CA265_RS11620 at 2881.514 kb on - strand, within CA265_RS11620 at 2881.514 kb on - strand, within CA265_RS11620 at 2881.542 kb on + strand, within CA265_RS11620 at 2881.542 kb on + strand, within CA265_RS11620 at 2881.641 kb on + strand, within CA265_RS11620 at 2881.693 kb on + strand, within CA265_RS11620 at 2881.710 kb on + strand, within CA265_RS11620 at 2881.726 kb on - strand at 2881.748 kb on - strand, within CA265_RS11620 at 2881.750 kb on - strand, within CA265_RS11620 at 2881.750 kb on - strand, within CA265_RS11620 at 2881.797 kb on - strand, within CA265_RS11620 at 2881.802 kb on - strand, within CA265_RS11620 at 2881.872 kb on - strand, within CA265_RS11620 at 2881.891 kb on + strand, within CA265_RS11620 at 2881.891 kb on + strand, within CA265_RS11620 at 2881.917 kb on + strand, within CA265_RS11620 at 2881.958 kb on + strand, within CA265_RS11620 at 2881.959 kb on - strand, within CA265_RS11620 at 2881.959 kb on - strand, within CA265_RS11620 at 2881.972 kb on - strand, within CA265_RS11620 at 2881.991 kb on + strand, within CA265_RS11620 at 2881.996 kb on - strand, within CA265_RS11620 at 2882.006 kb on - strand, within CA265_RS11620 at 2882.006 kb on - strand at 2882.150 kb on + strand at 2882.150 kb on + strand at 2882.172 kb on - strand at 2882.298 kb on - strand at 2882.320 kb on - strand at 2882.321 kb on + strand at 2882.321 kb on + strand at 2882.348 kb on + strand at 2882.350 kb on + strand at 2882.350 kb on + strand at 2882.351 kb on - strand at 2882.466 kb on + strand at 2882.467 kb on - strand at 2882.484 kb on + strand at 2882.485 kb on - strand at 2882.485 kb on - strand at 2882.508 kb on + strand at 2882.513 kb on + strand at 2882.513 kb on + strand at 2882.543 kb on + strand at 2882.543 kb on + strand at 2882.622 kb on + strand, within CA265_RS11625 at 2882.624 kb on + strand, within CA265_RS11625 at 2882.624 kb on + strand, within CA265_RS11625 at 2882.628 kb on + strand, within CA265_RS11625 at 2882.629 kb on - strand, within CA265_RS11625 at 2882.629 kb on - strand, within CA265_RS11625 at 2882.688 kb on + strand, within CA265_RS11625 at 2882.688 kb on + strand, within CA265_RS11625 at 2882.689 kb on - strand, within CA265_RS11625 at 2882.705 kb on + strand, within CA265_RS11625 at 2882.716 kb on - strand, within CA265_RS11625 at 2882.859 kb on + strand, within CA265_RS11625 at 2882.860 kb on - strand, within CA265_RS11625 at 2882.860 kb on - strand, within CA265_RS11625 at 2882.947 kb on + strand, within CA265_RS11625 at 2882.948 kb on - strand, within CA265_RS11625 at 2883.061 kb on - strand, within CA265_RS11625 at 2883.085 kb on + strand, within CA265_RS11625 at 2883.110 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.02 mg/ml remove 2,881,307 + CA265_RS11620 0.11 -1.2 2,881,311 + CA265_RS11620 0.11 -0.1 2,881,311 + CA265_RS11620 0.11 -1.5 2,881,314 + CA265_RS11620 0.11 -0.9 2,881,335 + CA265_RS11620 0.14 -1.2 2,881,335 + CA265_RS11620 0.14 -1.3 2,881,336 - CA265_RS11620 0.14 -0.6 2,881,336 - CA265_RS11620 0.14 -1.5 2,881,336 - CA265_RS11620 0.14 -0.9 2,881,371 - CA265_RS11620 0.17 -1.0 2,881,411 + CA265_RS11620 0.21 -1.3 2,881,470 - CA265_RS11620 0.28 -1.3 2,881,513 + CA265_RS11620 0.32 -0.8 2,881,513 + CA265_RS11620 0.32 -1.9 2,881,514 - CA265_RS11620 0.32 -0.7 2,881,514 - CA265_RS11620 0.32 -0.7 2,881,514 - CA265_RS11620 0.32 -1.5 2,881,542 + CA265_RS11620 0.35 -1.5 2,881,542 + CA265_RS11620 0.35 -0.2 2,881,641 + CA265_RS11620 0.45 -0.1 2,881,693 + CA265_RS11620 0.51 -1.9 2,881,710 + CA265_RS11620 0.53 -0.5 2,881,726 - +2.1 2,881,748 - CA265_RS11620 0.56 -1.1 2,881,750 - CA265_RS11620 0.57 -0.8 2,881,750 - CA265_RS11620 0.57 -1.1 2,881,797 - CA265_RS11620 0.62 -0.3 2,881,802 - CA265_RS11620 0.62 -1.4 2,881,872 - CA265_RS11620 0.69 -1.3 2,881,891 + CA265_RS11620 0.71 -0.5 2,881,891 + CA265_RS11620 0.71 -1.1 2,881,917 + CA265_RS11620 0.74 -0.7 2,881,958 + CA265_RS11620 0.78 -0.8 2,881,959 - CA265_RS11620 0.78 +2.3 2,881,959 - CA265_RS11620 0.78 -1.2 2,881,972 - CA265_RS11620 0.80 -2.5 2,881,991 + CA265_RS11620 0.82 -0.8 2,881,996 - CA265_RS11620 0.82 -0.2 2,882,006 - CA265_RS11620 0.83 -0.5 2,882,006 - +2.6 2,882,150 + +0.2 2,882,150 + -0.2 2,882,172 - -0.3 2,882,298 - -0.6 2,882,320 - -0.0 2,882,321 + +0.2 2,882,321 + -0.3 2,882,348 + -0.0 2,882,350 + -0.1 2,882,350 + +0.0 2,882,351 - -0.3 2,882,466 + +0.3 2,882,467 - -0.1 2,882,484 + +0.4 2,882,485 - +0.4 2,882,485 - -0.1 2,882,508 + -0.4 2,882,513 + +0.7 2,882,513 + -1.4 2,882,543 + -0.6 2,882,543 + +0.6 2,882,622 + CA265_RS11625 0.14 +0.5 2,882,624 + CA265_RS11625 0.15 -2.3 2,882,624 + CA265_RS11625 0.15 +0.1 2,882,628 + CA265_RS11625 0.15 -0.6 2,882,629 - CA265_RS11625 0.16 -0.6 2,882,629 - CA265_RS11625 0.16 +1.1 2,882,688 + CA265_RS11625 0.25 -0.2 2,882,688 + CA265_RS11625 0.25 +0.1 2,882,689 - CA265_RS11625 0.25 -0.1 2,882,705 + CA265_RS11625 0.28 -0.3 2,882,716 - CA265_RS11625 0.30 -0.0 2,882,859 + CA265_RS11625 0.53 +0.2 2,882,860 - CA265_RS11625 0.53 +0.3 2,882,860 - CA265_RS11625 0.53 -1.3 2,882,947 + CA265_RS11625 0.67 -0.1 2,882,948 - CA265_RS11625 0.67 -0.0 2,883,061 - CA265_RS11625 0.85 -0.4 2,883,085 + CA265_RS11625 0.89 -0.2 2,883,110 - +0.1
Or see this region's nucleotide sequence