Experiment: R2A with A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS10950 and CA265_RS10955 are separated by 370 nucleotides CA265_RS10955 and CA265_RS10960 overlap by 17 nucleotides CA265_RS10960 and CA265_RS10965 are separated by 67 nucleotides CA265_RS10965 and CA265_RS10970 are separated by 331 nucleotides
CA265_RS10950: CA265_RS10950 - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB, at 2,694,579 to 2,695,274
_RS10950
CA265_RS10955: CA265_RS10955 - membrane protein insertion efficiency factor YidD, at 2,695,645 to 2,695,875
_RS10955
CA265_RS10960: CA265_RS10960 - ribonuclease P protein component, at 2,695,859 to 2,696,257
_RS10960
CA265_RS10965: CA265_RS10965 - 50S ribosomal protein L34, at 2,696,325 to 2,696,483
_RS10965
CA265_RS10970: CA265_RS10970 - hypothetical protein, at 2,696,815 to 2,697,150
_RS10970
Position (kb)
2695
2696 Strain fitness (log2 ratio)
-2
-1
0
1 at 2695.286 kb on - strand at 2695.289 kb on - strand at 2695.289 kb on - strand at 2695.460 kb on - strand at 2695.485 kb on - strand at 2695.517 kb on + strand at 2695.543 kb on + strand at 2695.543 kb on + strand at 2695.593 kb on + strand at 2695.633 kb on + strand at 2695.637 kb on - strand at 2695.637 kb on - strand at 2695.722 kb on + strand, within CA265_RS10955 at 2695.820 kb on + strand, within CA265_RS10955 at 2695.823 kb on - strand, within CA265_RS10955 at 2695.827 kb on + strand, within CA265_RS10955 at 2696.258 kb on - strand at 2696.626 kb on + strand at 2696.669 kb on - strand at 2696.671 kb on + strand at 2696.671 kb on + strand at 2696.675 kb on + strand at 2696.675 kb on + strand at 2696.698 kb on - strand at 2696.797 kb on - strand at 2696.799 kb on + strand at 2696.841 kb on + strand at 2696.842 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.02 mg/ml remove 2,695,286 - -0.4 2,695,289 - -0.1 2,695,289 - +0.5 2,695,460 - -0.3 2,695,485 - -0.3 2,695,517 + -0.1 2,695,543 + -0.3 2,695,543 + -0.5 2,695,593 + -1.2 2,695,633 + +0.9 2,695,637 - -0.3 2,695,637 - -0.2 2,695,722 + CA265_RS10955 0.33 -1.3 2,695,820 + CA265_RS10955 0.76 -0.4 2,695,823 - CA265_RS10955 0.77 -1.3 2,695,827 + CA265_RS10955 0.79 +0.5 2,696,258 - -0.7 2,696,626 + -0.5 2,696,669 - -2.5 2,696,671 + +0.2 2,696,671 + +0.6 2,696,675 + +0.2 2,696,675 + -0.2 2,696,698 - -0.9 2,696,797 - -0.1 2,696,799 + +0.0 2,696,841 + -0.1 2,696,842 - +0.3
Or see this region's nucleotide sequence