Experiment: R2A with A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS10525 and CA265_RS10530 are separated by 284 nucleotides CA265_RS10530 and CA265_RS10535 are separated by 46 nucleotides
CA265_RS10525: CA265_RS10525 - sialidase, at 2,605,281 to 2,606,321
_RS10525
CA265_RS10530: CA265_RS10530 - ribonuclease R, at 2,606,606 to 2,608,738
_RS10530
CA265_RS10535: CA265_RS10535 - isoprenyl synthetase, at 2,608,785 to 2,609,759
_RS10535
Position (kb)
2606
2607
2608
2609 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2605.615 kb on + strand, within CA265_RS10525 at 2605.616 kb on - strand, within CA265_RS10525 at 2605.627 kb on - strand, within CA265_RS10525 at 2605.638 kb on - strand, within CA265_RS10525 at 2605.719 kb on - strand, within CA265_RS10525 at 2605.763 kb on + strand, within CA265_RS10525 at 2605.764 kb on - strand, within CA265_RS10525 at 2605.883 kb on + strand, within CA265_RS10525 at 2605.908 kb on - strand, within CA265_RS10525 at 2605.940 kb on - strand, within CA265_RS10525 at 2606.062 kb on + strand, within CA265_RS10525 at 2606.062 kb on + strand, within CA265_RS10525 at 2606.063 kb on - strand, within CA265_RS10525 at 2606.088 kb on + strand, within CA265_RS10525 at 2606.090 kb on + strand, within CA265_RS10525 at 2606.132 kb on + strand, within CA265_RS10525 at 2606.182 kb on - strand, within CA265_RS10525 at 2606.189 kb on - strand, within CA265_RS10525 at 2606.211 kb on + strand, within CA265_RS10525 at 2606.251 kb on + strand at 2606.263 kb on - strand at 2606.264 kb on + strand at 2606.265 kb on - strand at 2606.285 kb on + strand at 2606.286 kb on - strand at 2606.300 kb on + strand at 2606.376 kb on - strand at 2606.379 kb on - strand at 2606.394 kb on - strand at 2606.461 kb on + strand at 2606.518 kb on + strand at 2606.554 kb on - strand at 2606.600 kb on + strand at 2606.601 kb on - strand at 2606.605 kb on + strand at 2606.692 kb on + strand at 2606.840 kb on - strand, within CA265_RS10530 at 2606.970 kb on - strand, within CA265_RS10530 at 2607.026 kb on - strand, within CA265_RS10530 at 2607.026 kb on - strand, within CA265_RS10530 at 2607.055 kb on - strand, within CA265_RS10530 at 2607.055 kb on - strand, within CA265_RS10530 at 2607.229 kb on - strand, within CA265_RS10530 at 2607.268 kb on + strand, within CA265_RS10530 at 2607.269 kb on - strand, within CA265_RS10530 at 2607.281 kb on + strand, within CA265_RS10530 at 2607.403 kb on - strand, within CA265_RS10530 at 2607.503 kb on + strand, within CA265_RS10530 at 2607.503 kb on + strand, within CA265_RS10530 at 2607.539 kb on + strand, within CA265_RS10530 at 2607.540 kb on - strand, within CA265_RS10530 at 2607.584 kb on - strand, within CA265_RS10530 at 2607.584 kb on - strand, within CA265_RS10530 at 2607.604 kb on + strand, within CA265_RS10530 at 2607.638 kb on - strand, within CA265_RS10530 at 2607.667 kb on - strand, within CA265_RS10530 at 2607.918 kb on + strand, within CA265_RS10530 at 2607.928 kb on + strand, within CA265_RS10530 at 2607.956 kb on + strand, within CA265_RS10530 at 2607.957 kb on - strand, within CA265_RS10530 at 2608.168 kb on - strand, within CA265_RS10530 at 2608.174 kb on + strand, within CA265_RS10530 at 2608.194 kb on + strand, within CA265_RS10530 at 2608.231 kb on - strand, within CA265_RS10530 at 2608.232 kb on + strand, within CA265_RS10530 at 2608.240 kb on + strand, within CA265_RS10530 at 2608.277 kb on + strand, within CA265_RS10530 at 2608.277 kb on + strand, within CA265_RS10530 at 2608.278 kb on - strand, within CA265_RS10530 at 2608.318 kb on - strand, within CA265_RS10530 at 2608.335 kb on + strand, within CA265_RS10530 at 2608.343 kb on + strand, within CA265_RS10530 at 2608.347 kb on + strand, within CA265_RS10530 at 2608.347 kb on + strand, within CA265_RS10530 at 2608.348 kb on - strand, within CA265_RS10530 at 2608.348 kb on - strand, within CA265_RS10530 at 2608.444 kb on + strand, within CA265_RS10530 at 2608.471 kb on - strand, within CA265_RS10530 at 2608.532 kb on + strand at 2608.533 kb on - strand at 2608.584 kb on + strand at 2608.585 kb on - strand at 2608.585 kb on - strand at 2608.585 kb on - strand at 2608.625 kb on - strand at 2608.781 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.02 mg/ml remove 2,605,615 + CA265_RS10525 0.32 -0.1 2,605,616 - CA265_RS10525 0.32 +0.8 2,605,627 - CA265_RS10525 0.33 +0.4 2,605,638 - CA265_RS10525 0.34 -0.2 2,605,719 - CA265_RS10525 0.42 +0.0 2,605,763 + CA265_RS10525 0.46 -0.2 2,605,764 - CA265_RS10525 0.46 +0.9 2,605,883 + CA265_RS10525 0.58 +0.6 2,605,908 - CA265_RS10525 0.60 -0.1 2,605,940 - CA265_RS10525 0.63 -0.1 2,606,062 + CA265_RS10525 0.75 +0.1 2,606,062 + CA265_RS10525 0.75 +0.6 2,606,063 - CA265_RS10525 0.75 -0.2 2,606,088 + CA265_RS10525 0.78 -0.5 2,606,090 + CA265_RS10525 0.78 -0.9 2,606,132 + CA265_RS10525 0.82 +0.0 2,606,182 - CA265_RS10525 0.87 +0.2 2,606,189 - CA265_RS10525 0.87 -0.2 2,606,211 + CA265_RS10525 0.89 +0.8 2,606,251 + +0.5 2,606,263 - -0.6 2,606,264 + -0.5 2,606,265 - +0.1 2,606,285 + +0.2 2,606,286 - -0.2 2,606,300 + -0.0 2,606,376 - -0.0 2,606,379 - -0.6 2,606,394 - -1.2 2,606,461 + -0.4 2,606,518 + -0.7 2,606,554 - -0.0 2,606,600 + +0.2 2,606,601 - -1.7 2,606,605 + -0.9 2,606,692 + -0.4 2,606,840 - CA265_RS10530 0.11 -1.8 2,606,970 - CA265_RS10530 0.17 -1.3 2,607,026 - CA265_RS10530 0.20 -0.8 2,607,026 - CA265_RS10530 0.20 -0.8 2,607,055 - CA265_RS10530 0.21 +0.2 2,607,055 - CA265_RS10530 0.21 -0.8 2,607,229 - CA265_RS10530 0.29 -1.4 2,607,268 + CA265_RS10530 0.31 -0.6 2,607,269 - CA265_RS10530 0.31 +0.5 2,607,281 + CA265_RS10530 0.32 +1.2 2,607,403 - CA265_RS10530 0.37 -1.3 2,607,503 + CA265_RS10530 0.42 -0.6 2,607,503 + CA265_RS10530 0.42 -0.2 2,607,539 + CA265_RS10530 0.44 +0.2 2,607,540 - CA265_RS10530 0.44 -0.8 2,607,584 - CA265_RS10530 0.46 -0.1 2,607,584 - CA265_RS10530 0.46 -0.2 2,607,604 + CA265_RS10530 0.47 -2.6 2,607,638 - CA265_RS10530 0.48 -2.0 2,607,667 - CA265_RS10530 0.50 -0.6 2,607,918 + CA265_RS10530 0.62 -1.1 2,607,928 + CA265_RS10530 0.62 -0.6 2,607,956 + CA265_RS10530 0.63 +0.0 2,607,957 - CA265_RS10530 0.63 -1.1 2,608,168 - CA265_RS10530 0.73 -0.2 2,608,174 + CA265_RS10530 0.74 +0.2 2,608,194 + CA265_RS10530 0.74 -1.0 2,608,231 - CA265_RS10530 0.76 -3.5 2,608,232 + CA265_RS10530 0.76 -1.8 2,608,240 + CA265_RS10530 0.77 +0.6 2,608,277 + CA265_RS10530 0.78 -0.5 2,608,277 + CA265_RS10530 0.78 +0.2 2,608,278 - CA265_RS10530 0.78 +1.2 2,608,318 - CA265_RS10530 0.80 -1.2 2,608,335 + CA265_RS10530 0.81 -3.6 2,608,343 + CA265_RS10530 0.81 -1.5 2,608,347 + CA265_RS10530 0.82 -0.9 2,608,347 + CA265_RS10530 0.82 -1.1 2,608,348 - CA265_RS10530 0.82 -0.1 2,608,348 - CA265_RS10530 0.82 -0.2 2,608,444 + CA265_RS10530 0.86 -0.9 2,608,471 - CA265_RS10530 0.87 -0.6 2,608,532 + +0.7 2,608,533 - -0.7 2,608,584 + -1.3 2,608,585 - -2.8 2,608,585 - -1.5 2,608,585 - -0.9 2,608,625 - -0.6 2,608,781 + -0.8
Or see this region's nucleotide sequence