Experiment: R2A with A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS09730 and CA265_RS09735 are separated by 91 nucleotides CA265_RS09735 and CA265_RS09740 are separated by 133 nucleotides CA265_RS09740 and CA265_RS09745 are separated by 79 nucleotides CA265_RS09745 and CA265_RS09750 are separated by 67 nucleotides
CA265_RS09730: CA265_RS09730 - subclass B3 metallo-beta-lactamase, at 2,401,150 to 2,402,007
_RS09730
CA265_RS09735: CA265_RS09735 - nuclease, at 2,402,099 to 2,402,449
_RS09735
CA265_RS09740: CA265_RS09740 - transcriptional regulator, at 2,402,583 to 2,403,359
_RS09740
CA265_RS09745: CA265_RS09745 - hypothetical protein, at 2,403,439 to 2,403,891
_RS09745
CA265_RS09750: CA265_RS09750 - SAM-dependent methyltransferase, at 2,403,959 to 2,404,693
_RS09750
Position (kb)
2402
2403
2404 Strain fitness (log2 ratio)
-2
-1
0
1 at 2401.635 kb on - strand, within CA265_RS09730 at 2401.645 kb on - strand, within CA265_RS09730 at 2401.645 kb on - strand, within CA265_RS09730 at 2401.650 kb on - strand, within CA265_RS09730 at 2401.674 kb on + strand, within CA265_RS09730 at 2401.696 kb on - strand, within CA265_RS09730 at 2401.698 kb on - strand, within CA265_RS09730 at 2401.698 kb on - strand, within CA265_RS09730 at 2401.702 kb on - strand, within CA265_RS09730 at 2401.747 kb on - strand, within CA265_RS09730 at 2401.758 kb on - strand, within CA265_RS09730 at 2401.785 kb on - strand, within CA265_RS09730 at 2401.792 kb on + strand, within CA265_RS09730 at 2401.813 kb on + strand, within CA265_RS09730 at 2401.816 kb on - strand, within CA265_RS09730 at 2401.896 kb on + strand, within CA265_RS09730 at 2401.942 kb on - strand at 2402.014 kb on - strand at 2402.039 kb on + strand at 2402.179 kb on + strand, within CA265_RS09735 at 2402.180 kb on - strand, within CA265_RS09735 at 2402.213 kb on + strand, within CA265_RS09735 at 2402.329 kb on + strand, within CA265_RS09735 at 2402.404 kb on + strand, within CA265_RS09735 at 2402.405 kb on - strand, within CA265_RS09735 at 2402.405 kb on - strand, within CA265_RS09735 at 2402.410 kb on + strand, within CA265_RS09735 at 2402.410 kb on + strand, within CA265_RS09735 at 2402.432 kb on - strand at 2402.484 kb on - strand at 2403.131 kb on + strand, within CA265_RS09740 at 2403.256 kb on + strand, within CA265_RS09740 at 2403.398 kb on + strand at 2403.403 kb on - strand at 2403.406 kb on + strand at 2403.436 kb on - strand at 2403.473 kb on + strand at 2403.889 kb on + strand at 2403.894 kb on - strand at 2403.983 kb on + strand at 2404.092 kb on - strand, within CA265_RS09750 at 2404.092 kb on - strand, within CA265_RS09750 at 2404.126 kb on - strand, within CA265_RS09750 at 2404.292 kb on + strand, within CA265_RS09750 at 2404.293 kb on - strand, within CA265_RS09750
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.02 mg/ml remove 2,401,635 - CA265_RS09730 0.57 +0.4 2,401,645 - CA265_RS09730 0.58 +0.9 2,401,645 - CA265_RS09730 0.58 +0.1 2,401,650 - CA265_RS09730 0.58 +1.6 2,401,674 + CA265_RS09730 0.61 -0.7 2,401,696 - CA265_RS09730 0.64 +0.1 2,401,698 - CA265_RS09730 0.64 -0.4 2,401,698 - CA265_RS09730 0.64 +0.2 2,401,702 - CA265_RS09730 0.64 -0.3 2,401,747 - CA265_RS09730 0.70 +0.8 2,401,758 - CA265_RS09730 0.71 +1.0 2,401,785 - CA265_RS09730 0.74 -0.3 2,401,792 + CA265_RS09730 0.75 +0.5 2,401,813 + CA265_RS09730 0.77 -0.4 2,401,816 - CA265_RS09730 0.78 +0.2 2,401,896 + CA265_RS09730 0.87 -0.4 2,401,942 - -0.1 2,402,014 - -1.3 2,402,039 + +0.5 2,402,179 + CA265_RS09735 0.23 -0.3 2,402,180 - CA265_RS09735 0.23 -0.0 2,402,213 + CA265_RS09735 0.32 -0.5 2,402,329 + CA265_RS09735 0.66 -0.2 2,402,404 + CA265_RS09735 0.87 -0.1 2,402,405 - CA265_RS09735 0.87 +1.0 2,402,405 - CA265_RS09735 0.87 -0.8 2,402,410 + CA265_RS09735 0.89 -0.9 2,402,410 + CA265_RS09735 0.89 -0.6 2,402,432 - +0.0 2,402,484 - -0.1 2,403,131 + CA265_RS09740 0.71 +0.1 2,403,256 + CA265_RS09740 0.87 -2.4 2,403,398 + -0.8 2,403,403 - +0.4 2,403,406 + -1.3 2,403,436 - -0.1 2,403,473 + -0.8 2,403,889 + +0.1 2,403,894 - +0.1 2,403,983 + +1.7 2,404,092 - CA265_RS09750 0.18 -2.0 2,404,092 - CA265_RS09750 0.18 -0.5 2,404,126 - CA265_RS09750 0.23 +0.2 2,404,292 + CA265_RS09750 0.45 +0.2 2,404,293 - CA265_RS09750 0.45 +0.7
Or see this region's nucleotide sequence