Experiment: R2A with A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS09380 and CA265_RS09385 are separated by 144 nucleotides CA265_RS09385 and CA265_RS09390 overlap by 1 nucleotides CA265_RS09390 and CA265_RS09395 are separated by 57 nucleotides
CA265_RS09380: CA265_RS09380 - hypothetical protein, at 2,328,343 to 2,329,617
_RS09380
CA265_RS09385: CA265_RS09385 - hypothetical protein, at 2,329,762 to 2,330,211
_RS09385
CA265_RS09390: CA265_RS09390 - hypothetical protein, at 2,330,211 to 2,330,741
_RS09390
CA265_RS09395: CA265_RS09395 - hypothetical protein, at 2,330,799 to 2,331,269
_RS09395
Position (kb)
2329
2330
2331 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2328.768 kb on + strand, within CA265_RS09380 at 2328.773 kb on + strand, within CA265_RS09380 at 2328.784 kb on - strand, within CA265_RS09380 at 2328.784 kb on - strand, within CA265_RS09380 at 2328.804 kb on + strand, within CA265_RS09380 at 2328.804 kb on + strand, within CA265_RS09380 at 2328.808 kb on - strand, within CA265_RS09380 at 2328.826 kb on + strand, within CA265_RS09380 at 2328.827 kb on - strand, within CA265_RS09380 at 2328.848 kb on + strand, within CA265_RS09380 at 2328.849 kb on - strand, within CA265_RS09380 at 2328.849 kb on - strand, within CA265_RS09380 at 2328.868 kb on + strand, within CA265_RS09380 at 2328.880 kb on + strand, within CA265_RS09380 at 2328.930 kb on + strand, within CA265_RS09380 at 2328.931 kb on - strand, within CA265_RS09380 at 2328.952 kb on + strand, within CA265_RS09380 at 2328.952 kb on + strand, within CA265_RS09380 at 2328.958 kb on + strand, within CA265_RS09380 at 2328.965 kb on + strand, within CA265_RS09380 at 2328.965 kb on + strand, within CA265_RS09380 at 2328.966 kb on - strand, within CA265_RS09380 at 2328.969 kb on + strand, within CA265_RS09380 at 2328.999 kb on + strand, within CA265_RS09380 at 2328.999 kb on + strand, within CA265_RS09380 at 2329.008 kb on + strand, within CA265_RS09380 at 2329.052 kb on + strand, within CA265_RS09380 at 2329.076 kb on + strand, within CA265_RS09380 at 2329.098 kb on + strand, within CA265_RS09380 at 2329.176 kb on + strand, within CA265_RS09380 at 2329.176 kb on + strand, within CA265_RS09380 at 2329.190 kb on - strand, within CA265_RS09380 at 2329.234 kb on + strand, within CA265_RS09380 at 2329.234 kb on + strand, within CA265_RS09380 at 2329.235 kb on - strand, within CA265_RS09380 at 2329.235 kb on - strand, within CA265_RS09380 at 2329.260 kb on + strand, within CA265_RS09380 at 2329.261 kb on - strand, within CA265_RS09380 at 2329.264 kb on + strand, within CA265_RS09380 at 2329.282 kb on + strand, within CA265_RS09380 at 2329.303 kb on + strand, within CA265_RS09380 at 2329.371 kb on + strand, within CA265_RS09380 at 2329.373 kb on + strand, within CA265_RS09380 at 2329.465 kb on + strand, within CA265_RS09380 at 2329.608 kb on + strand at 2329.609 kb on - strand at 2329.612 kb on - strand at 2329.706 kb on + strand at 2329.821 kb on + strand, within CA265_RS09385 at 2329.821 kb on + strand, within CA265_RS09385 at 2329.878 kb on - strand, within CA265_RS09385 at 2329.892 kb on - strand, within CA265_RS09385 at 2329.915 kb on - strand, within CA265_RS09385 at 2329.944 kb on + strand, within CA265_RS09385 at 2329.953 kb on + strand, within CA265_RS09385 at 2330.036 kb on - strand, within CA265_RS09385 at 2330.036 kb on - strand, within CA265_RS09385 at 2330.131 kb on + strand, within CA265_RS09385 at 2330.135 kb on + strand, within CA265_RS09385 at 2330.213 kb on - strand at 2330.225 kb on + strand at 2330.225 kb on + strand at 2330.226 kb on - strand at 2330.235 kb on - strand at 2330.245 kb on + strand at 2330.246 kb on - strand at 2330.251 kb on - strand at 2330.262 kb on - strand at 2330.285 kb on + strand, within CA265_RS09390 at 2330.347 kb on - strand, within CA265_RS09390 at 2330.351 kb on + strand, within CA265_RS09390 at 2330.381 kb on + strand, within CA265_RS09390 at 2330.423 kb on + strand, within CA265_RS09390 at 2330.425 kb on + strand, within CA265_RS09390 at 2330.425 kb on + strand, within CA265_RS09390 at 2330.425 kb on + strand, within CA265_RS09390 at 2330.426 kb on - strand, within CA265_RS09390 at 2330.426 kb on - strand, within CA265_RS09390 at 2330.426 kb on - strand, within CA265_RS09390 at 2330.467 kb on + strand, within CA265_RS09390 at 2330.468 kb on - strand, within CA265_RS09390 at 2330.514 kb on + strand, within CA265_RS09390 at 2330.565 kb on + strand, within CA265_RS09390 at 2330.566 kb on - strand, within CA265_RS09390 at 2330.644 kb on - strand, within CA265_RS09390 at 2330.658 kb on + strand, within CA265_RS09390 at 2330.659 kb on - strand, within CA265_RS09390 at 2330.735 kb on + strand at 2330.899 kb on + strand, within CA265_RS09395 at 2330.899 kb on + strand, within CA265_RS09395 at 2330.980 kb on - strand, within CA265_RS09395 at 2331.011 kb on + strand, within CA265_RS09395 at 2331.015 kb on - strand, within CA265_RS09395 at 2331.048 kb on - strand, within CA265_RS09395 at 2331.186 kb on - strand, within CA265_RS09395
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.02 mg/ml remove 2,328,768 + CA265_RS09380 0.33 +0.1 2,328,773 + CA265_RS09380 0.34 -0.0 2,328,784 - CA265_RS09380 0.35 -1.8 2,328,784 - CA265_RS09380 0.35 -0.0 2,328,804 + CA265_RS09380 0.36 -0.2 2,328,804 + CA265_RS09380 0.36 -0.5 2,328,808 - CA265_RS09380 0.36 -0.4 2,328,826 + CA265_RS09380 0.38 -0.2 2,328,827 - CA265_RS09380 0.38 -0.3 2,328,848 + CA265_RS09380 0.40 +0.1 2,328,849 - CA265_RS09380 0.40 -1.2 2,328,849 - CA265_RS09380 0.40 -1.4 2,328,868 + CA265_RS09380 0.41 -0.2 2,328,880 + CA265_RS09380 0.42 +1.0 2,328,930 + CA265_RS09380 0.46 +0.5 2,328,931 - CA265_RS09380 0.46 -1.2 2,328,952 + CA265_RS09380 0.48 -0.9 2,328,952 + CA265_RS09380 0.48 -1.5 2,328,958 + CA265_RS09380 0.48 -0.6 2,328,965 + CA265_RS09380 0.49 +0.2 2,328,965 + CA265_RS09380 0.49 +0.3 2,328,966 - CA265_RS09380 0.49 -0.8 2,328,969 + CA265_RS09380 0.49 -0.1 2,328,999 + CA265_RS09380 0.51 -0.0 2,328,999 + CA265_RS09380 0.51 +0.3 2,329,008 + CA265_RS09380 0.52 +0.6 2,329,052 + CA265_RS09380 0.56 -0.4 2,329,076 + CA265_RS09380 0.57 +0.3 2,329,098 + CA265_RS09380 0.59 -0.4 2,329,176 + CA265_RS09380 0.65 +0.4 2,329,176 + CA265_RS09380 0.65 +0.1 2,329,190 - CA265_RS09380 0.66 -0.7 2,329,234 + CA265_RS09380 0.70 -0.6 2,329,234 + CA265_RS09380 0.70 -0.2 2,329,235 - CA265_RS09380 0.70 -0.6 2,329,235 - CA265_RS09380 0.70 -1.3 2,329,260 + CA265_RS09380 0.72 +1.1 2,329,261 - CA265_RS09380 0.72 -0.9 2,329,264 + CA265_RS09380 0.72 +0.9 2,329,282 + CA265_RS09380 0.74 +0.3 2,329,303 + CA265_RS09380 0.75 +1.0 2,329,371 + CA265_RS09380 0.81 +2.2 2,329,373 + CA265_RS09380 0.81 +0.1 2,329,465 + CA265_RS09380 0.88 -0.1 2,329,608 + -0.3 2,329,609 - -0.9 2,329,612 - -0.9 2,329,706 + -0.5 2,329,821 + CA265_RS09385 0.13 -0.1 2,329,821 + CA265_RS09385 0.13 -0.2 2,329,878 - CA265_RS09385 0.26 -1.6 2,329,892 - CA265_RS09385 0.29 -3.2 2,329,915 - CA265_RS09385 0.34 -1.9 2,329,944 + CA265_RS09385 0.40 -0.4 2,329,953 + CA265_RS09385 0.42 -0.0 2,330,036 - CA265_RS09385 0.61 -0.1 2,330,036 - CA265_RS09385 0.61 -0.8 2,330,131 + CA265_RS09385 0.82 -1.1 2,330,135 + CA265_RS09385 0.83 -0.1 2,330,213 - -0.8 2,330,225 + -0.2 2,330,225 + +1.0 2,330,226 - -1.3 2,330,235 - -2.9 2,330,245 + -0.6 2,330,246 - -0.2 2,330,251 - -1.6 2,330,262 - -1.7 2,330,285 + CA265_RS09390 0.14 -0.2 2,330,347 - CA265_RS09390 0.26 -1.1 2,330,351 + CA265_RS09390 0.26 +0.3 2,330,381 + CA265_RS09390 0.32 +0.3 2,330,423 + CA265_RS09390 0.40 -0.1 2,330,425 + CA265_RS09390 0.40 -0.3 2,330,425 + CA265_RS09390 0.40 +0.2 2,330,425 + CA265_RS09390 0.40 -0.5 2,330,426 - CA265_RS09390 0.40 -1.7 2,330,426 - CA265_RS09390 0.40 -1.9 2,330,426 - CA265_RS09390 0.40 -1.0 2,330,467 + CA265_RS09390 0.48 +0.4 2,330,468 - CA265_RS09390 0.48 -0.8 2,330,514 + CA265_RS09390 0.57 -0.7 2,330,565 + CA265_RS09390 0.67 -0.2 2,330,566 - CA265_RS09390 0.67 -0.3 2,330,644 - CA265_RS09390 0.82 +1.2 2,330,658 + CA265_RS09390 0.84 -0.9 2,330,659 - CA265_RS09390 0.84 -0.6 2,330,735 + +0.0 2,330,899 + CA265_RS09395 0.21 +0.3 2,330,899 + CA265_RS09395 0.21 -0.5 2,330,980 - CA265_RS09395 0.38 -0.2 2,331,011 + CA265_RS09395 0.45 -0.4 2,331,015 - CA265_RS09395 0.46 -2.9 2,331,048 - CA265_RS09395 0.53 -0.8 2,331,186 - CA265_RS09395 0.82 -0.6
Or see this region's nucleotide sequence