Experiment: R2A with A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS08905 and CA265_RS08910 are separated by 85 nucleotides CA265_RS08910 and CA265_RS08915 are separated by 2 nucleotides CA265_RS08915 and CA265_RS08920 are separated by 17 nucleotides
CA265_RS08905: CA265_RS08905 - DNA helicase RecQ, at 2,224,724 to 2,226,913
_RS08905
CA265_RS08910: CA265_RS08910 - 3-deoxy-8-phosphooctulonate synthase, at 2,226,999 to 2,227,811
_RS08910
CA265_RS08915: CA265_RS08915 - hypothetical protein, at 2,227,814 to 2,228,528
_RS08915
CA265_RS08920: CA265_RS08920 - hypothetical protein, at 2,228,546 to 2,228,962
_RS08920
Position (kb)
2226
2227
2228 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2226.162 kb on - strand, within CA265_RS08905 at 2226.223 kb on + strand, within CA265_RS08905 at 2226.237 kb on - strand, within CA265_RS08905 at 2226.325 kb on + strand, within CA265_RS08905 at 2226.325 kb on + strand, within CA265_RS08905 at 2226.477 kb on + strand, within CA265_RS08905 at 2226.577 kb on + strand, within CA265_RS08905 at 2226.903 kb on + strand at 2226.994 kb on + strand at 2226.995 kb on - strand at 2226.999 kb on - strand at 2227.012 kb on + strand at 2227.085 kb on + strand, within CA265_RS08910 at 2227.182 kb on - strand, within CA265_RS08910 at 2227.268 kb on + strand, within CA265_RS08910 at 2227.269 kb on - strand, within CA265_RS08910 at 2227.321 kb on + strand, within CA265_RS08910 at 2227.322 kb on - strand, within CA265_RS08910 at 2227.496 kb on + strand, within CA265_RS08910 at 2227.583 kb on + strand, within CA265_RS08910 at 2227.621 kb on + strand, within CA265_RS08910 at 2227.690 kb on + strand, within CA265_RS08910 at 2227.742 kb on + strand at 2227.743 kb on - strand at 2227.747 kb on + strand at 2227.775 kb on - strand at 2227.802 kb on + strand at 2227.802 kb on + strand at 2227.803 kb on - strand at 2227.872 kb on - strand at 2227.890 kb on + strand, within CA265_RS08915 at 2227.939 kb on + strand, within CA265_RS08915 at 2227.940 kb on - strand, within CA265_RS08915 at 2227.943 kb on + strand, within CA265_RS08915 at 2227.944 kb on - strand, within CA265_RS08915 at 2227.944 kb on - strand, within CA265_RS08915 at 2227.946 kb on + strand, within CA265_RS08915 at 2227.947 kb on - strand, within CA265_RS08915 at 2227.947 kb on - strand, within CA265_RS08915 at 2227.966 kb on + strand, within CA265_RS08915 at 2227.995 kb on + strand, within CA265_RS08915 at 2227.996 kb on - strand, within CA265_RS08915 at 2228.028 kb on + strand, within CA265_RS08915 at 2228.139 kb on + strand, within CA265_RS08915 at 2228.140 kb on - strand, within CA265_RS08915 at 2228.211 kb on + strand, within CA265_RS08915 at 2228.298 kb on + strand, within CA265_RS08915 at 2228.325 kb on - strand, within CA265_RS08915 at 2228.325 kb on - strand, within CA265_RS08915 at 2228.386 kb on - strand, within CA265_RS08915 at 2228.403 kb on - strand, within CA265_RS08915 at 2228.506 kb on + strand at 2228.510 kb on - strand at 2228.549 kb on + strand at 2228.631 kb on - strand, within CA265_RS08920 at 2228.631 kb on - strand, within CA265_RS08920 at 2228.633 kb on + strand, within CA265_RS08920 at 2228.633 kb on + strand, within CA265_RS08920 at 2228.643 kb on + strand, within CA265_RS08920 at 2228.690 kb on + strand, within CA265_RS08920 at 2228.691 kb on - strand, within CA265_RS08920 at 2228.691 kb on - strand, within CA265_RS08920 at 2228.725 kb on - strand, within CA265_RS08920 at 2228.732 kb on + strand, within CA265_RS08920
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.02 mg/ml remove 2,226,162 - CA265_RS08905 0.66 -1.8 2,226,223 + CA265_RS08905 0.68 +0.2 2,226,237 - CA265_RS08905 0.69 -0.4 2,226,325 + CA265_RS08905 0.73 +0.2 2,226,325 + CA265_RS08905 0.73 -0.1 2,226,477 + CA265_RS08905 0.80 +1.9 2,226,577 + CA265_RS08905 0.85 -0.4 2,226,903 + -2.1 2,226,994 + +0.8 2,226,995 - +0.2 2,226,999 - -2.1 2,227,012 + -2.6 2,227,085 + CA265_RS08910 0.11 -2.8 2,227,182 - CA265_RS08910 0.23 -1.7 2,227,268 + CA265_RS08910 0.33 -1.6 2,227,269 - CA265_RS08910 0.33 -1.4 2,227,321 + CA265_RS08910 0.40 -0.7 2,227,322 - CA265_RS08910 0.40 -1.3 2,227,496 + CA265_RS08910 0.61 -3.8 2,227,583 + CA265_RS08910 0.72 -1.8 2,227,621 + CA265_RS08910 0.77 -2.8 2,227,690 + CA265_RS08910 0.85 -2.3 2,227,742 + -1.9 2,227,743 - -2.8 2,227,747 + -1.8 2,227,775 - -2.1 2,227,802 + -0.4 2,227,802 + +1.1 2,227,803 - -2.1 2,227,872 - -0.6 2,227,890 + CA265_RS08915 0.11 -0.1 2,227,939 + CA265_RS08915 0.17 -0.2 2,227,940 - CA265_RS08915 0.18 -0.0 2,227,943 + CA265_RS08915 0.18 -0.1 2,227,944 - CA265_RS08915 0.18 -1.6 2,227,944 - CA265_RS08915 0.18 -1.1 2,227,946 + CA265_RS08915 0.18 +0.4 2,227,947 - CA265_RS08915 0.19 -0.7 2,227,947 - CA265_RS08915 0.19 -1.3 2,227,966 + CA265_RS08915 0.21 +0.3 2,227,995 + CA265_RS08915 0.25 -0.7 2,227,996 - CA265_RS08915 0.25 -0.3 2,228,028 + CA265_RS08915 0.30 +0.6 2,228,139 + CA265_RS08915 0.45 +1.0 2,228,140 - CA265_RS08915 0.46 +0.5 2,228,211 + CA265_RS08915 0.56 -0.5 2,228,298 + CA265_RS08915 0.68 +0.2 2,228,325 - CA265_RS08915 0.71 -0.2 2,228,325 - CA265_RS08915 0.71 -1.6 2,228,386 - CA265_RS08915 0.80 +0.4 2,228,403 - CA265_RS08915 0.82 -0.6 2,228,506 + +0.1 2,228,510 - +1.3 2,228,549 + +0.3 2,228,631 - CA265_RS08920 0.20 +0.6 2,228,631 - CA265_RS08920 0.20 -0.0 2,228,633 + CA265_RS08920 0.21 +0.7 2,228,633 + CA265_RS08920 0.21 -0.7 2,228,643 + CA265_RS08920 0.23 +0.2 2,228,690 + CA265_RS08920 0.35 -0.4 2,228,691 - CA265_RS08920 0.35 +0.2 2,228,691 - CA265_RS08920 0.35 -0.3 2,228,725 - CA265_RS08920 0.43 +0.8 2,228,732 + CA265_RS08920 0.45 +1.2
Or see this region's nucleotide sequence