Experiment: R2A with A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS06280 and CA265_RS06285 are separated by 1 nucleotides CA265_RS06285 and CA265_RS06290 are separated by 69 nucleotides CA265_RS06290 and CA265_RS06295 are separated by 1 nucleotides
CA265_RS06280: CA265_RS06280 - GNAT family N-acetyltransferase, at 1,543,379 to 1,543,846
_RS06280
CA265_RS06285: CA265_RS06285 - YggS family pyridoxal phosphate enzyme, at 1,543,848 to 1,544,549
_RS06285
CA265_RS06290: CA265_RS06290 - hypothetical protein, at 1,544,619 to 1,545,182
_RS06290
CA265_RS06295: CA265_RS06295 - endonuclease MutS2, at 1,545,184 to 1,547,562
_RS06295
Position (kb)
1544
1545
1546 Strain fitness (log2 ratio)
-2
-1
0
1 at 1543.642 kb on - strand, within CA265_RS06280 at 1543.673 kb on + strand, within CA265_RS06280 at 1543.682 kb on + strand, within CA265_RS06280 at 1543.682 kb on + strand, within CA265_RS06280 at 1543.686 kb on + strand, within CA265_RS06280 at 1543.686 kb on + strand, within CA265_RS06280 at 1543.797 kb on - strand, within CA265_RS06280 at 1543.939 kb on - strand, within CA265_RS06285 at 1543.950 kb on - strand, within CA265_RS06285 at 1544.078 kb on + strand, within CA265_RS06285 at 1544.115 kb on - strand, within CA265_RS06285 at 1544.145 kb on + strand, within CA265_RS06285 at 1544.203 kb on + strand, within CA265_RS06285 at 1544.215 kb on - strand, within CA265_RS06285 at 1544.294 kb on + strand, within CA265_RS06285 at 1544.294 kb on + strand, within CA265_RS06285 at 1544.295 kb on - strand, within CA265_RS06285 at 1544.327 kb on + strand, within CA265_RS06285 at 1544.337 kb on + strand, within CA265_RS06285 at 1544.348 kb on + strand, within CA265_RS06285 at 1544.349 kb on - strand, within CA265_RS06285 at 1544.387 kb on + strand, within CA265_RS06285 at 1544.440 kb on + strand, within CA265_RS06285 at 1544.440 kb on + strand, within CA265_RS06285 at 1544.440 kb on + strand, within CA265_RS06285 at 1544.460 kb on + strand, within CA265_RS06285 at 1544.522 kb on - strand at 1544.541 kb on + strand at 1545.119 kb on - strand, within CA265_RS06290 at 1545.153 kb on - strand at 1545.180 kb on + strand at 1545.188 kb on + strand at 1545.189 kb on - strand at 1545.258 kb on - strand at 1545.281 kb on + strand at 1545.349 kb on - strand at 1545.402 kb on + strand at 1545.471 kb on + strand, within CA265_RS06295 at 1545.483 kb on + strand, within CA265_RS06295 at 1545.527 kb on + strand, within CA265_RS06295 at 1545.529 kb on + strand, within CA265_RS06295 at 1545.654 kb on + strand, within CA265_RS06295 at 1545.691 kb on - strand, within CA265_RS06295 at 1545.747 kb on + strand, within CA265_RS06295 at 1545.777 kb on + strand, within CA265_RS06295 at 1545.777 kb on + strand, within CA265_RS06295 at 1545.778 kb on - strand, within CA265_RS06295 at 1545.799 kb on - strand, within CA265_RS06295 at 1545.886 kb on - strand, within CA265_RS06295 at 1545.888 kb on + strand, within CA265_RS06295 at 1545.888 kb on + strand, within CA265_RS06295 at 1545.947 kb on + strand, within CA265_RS06295 at 1545.989 kb on - strand, within CA265_RS06295 at 1546.074 kb on - strand, within CA265_RS06295 at 1546.097 kb on + strand, within CA265_RS06295 at 1546.161 kb on - strand, within CA265_RS06295 at 1546.164 kb on + strand, within CA265_RS06295 at 1546.164 kb on + strand, within CA265_RS06295 at 1546.170 kb on + strand, within CA265_RS06295
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.02 mg/ml remove 1,543,642 - CA265_RS06280 0.56 -0.5 1,543,673 + CA265_RS06280 0.63 -0.8 1,543,682 + CA265_RS06280 0.65 -0.3 1,543,682 + CA265_RS06280 0.65 -0.0 1,543,686 + CA265_RS06280 0.66 -1.1 1,543,686 + CA265_RS06280 0.66 +0.0 1,543,797 - CA265_RS06280 0.89 +0.3 1,543,939 - CA265_RS06285 0.13 -0.5 1,543,950 - CA265_RS06285 0.15 -0.5 1,544,078 + CA265_RS06285 0.33 -0.2 1,544,115 - CA265_RS06285 0.38 +0.9 1,544,145 + CA265_RS06285 0.42 +0.1 1,544,203 + CA265_RS06285 0.51 -0.3 1,544,215 - CA265_RS06285 0.52 -0.5 1,544,294 + CA265_RS06285 0.64 -1.8 1,544,294 + CA265_RS06285 0.64 +0.1 1,544,295 - CA265_RS06285 0.64 +0.1 1,544,327 + CA265_RS06285 0.68 -0.7 1,544,337 + CA265_RS06285 0.70 -1.0 1,544,348 + CA265_RS06285 0.71 +0.3 1,544,349 - CA265_RS06285 0.71 -1.2 1,544,387 + CA265_RS06285 0.77 -0.5 1,544,440 + CA265_RS06285 0.84 -1.7 1,544,440 + CA265_RS06285 0.84 -2.0 1,544,440 + CA265_RS06285 0.84 +0.2 1,544,460 + CA265_RS06285 0.87 -0.6 1,544,522 - -0.7 1,544,541 + -0.3 1,545,119 - CA265_RS06290 0.89 -1.7 1,545,153 - +0.2 1,545,180 + +0.7 1,545,188 + +0.7 1,545,189 - -0.9 1,545,258 - -0.2 1,545,281 + +0.3 1,545,349 - +0.6 1,545,402 + +0.4 1,545,471 + CA265_RS06295 0.12 -1.3 1,545,483 + CA265_RS06295 0.13 -0.9 1,545,527 + CA265_RS06295 0.14 -0.4 1,545,529 + CA265_RS06295 0.15 -1.8 1,545,654 + CA265_RS06295 0.20 -0.0 1,545,691 - CA265_RS06295 0.21 -0.2 1,545,747 + CA265_RS06295 0.24 +0.1 1,545,777 + CA265_RS06295 0.25 +0.1 1,545,777 + CA265_RS06295 0.25 -0.2 1,545,778 - CA265_RS06295 0.25 -0.4 1,545,799 - CA265_RS06295 0.26 +0.9 1,545,886 - CA265_RS06295 0.30 -0.5 1,545,888 + CA265_RS06295 0.30 -1.1 1,545,888 + CA265_RS06295 0.30 -0.6 1,545,947 + CA265_RS06295 0.32 +1.3 1,545,989 - CA265_RS06295 0.34 +1.1 1,546,074 - CA265_RS06295 0.37 +0.2 1,546,097 + CA265_RS06295 0.38 -0.9 1,546,161 - CA265_RS06295 0.41 +0.5 1,546,164 + CA265_RS06295 0.41 +0.3 1,546,164 + CA265_RS06295 0.41 -0.8 1,546,170 + CA265_RS06295 0.41 +0.0
Or see this region's nucleotide sequence