Experiment: R2A with A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS06245 and CA265_RS06250 are separated by 203 nucleotides CA265_RS06250 and CA265_RS06255 are separated by 6 nucleotides CA265_RS06255 and CA265_RS06260 are separated by 163 nucleotides
CA265_RS06245: CA265_RS06245 - hypothetical protein, at 1,537,328 to 1,537,546
_RS06245
CA265_RS06250: CA265_RS06250 - RNA polymerase subunit sigma-70, at 1,537,750 to 1,538,316
_RS06250
CA265_RS06255: CA265_RS06255 - serine protease, at 1,538,323 to 1,539,417
_RS06255
CA265_RS06260: CA265_RS06260 - peroxidase, at 1,539,581 to 1,540,216
_RS06260
Position (kb)
1538
1539
1540 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1537.725 kb on + strand at 1537.741 kb on + strand at 1537.808 kb on + strand, within CA265_RS06250 at 1537.849 kb on + strand, within CA265_RS06250 at 1538.058 kb on + strand, within CA265_RS06250 at 1538.241 kb on + strand, within CA265_RS06250 at 1538.342 kb on + strand at 1538.412 kb on - strand at 1538.454 kb on + strand, within CA265_RS06255 at 1538.455 kb on - strand, within CA265_RS06255 at 1538.520 kb on + strand, within CA265_RS06255 at 1538.521 kb on - strand, within CA265_RS06255 at 1538.605 kb on - strand, within CA265_RS06255 at 1538.664 kb on + strand, within CA265_RS06255 at 1538.994 kb on - strand, within CA265_RS06255 at 1539.008 kb on - strand, within CA265_RS06255 at 1539.036 kb on - strand, within CA265_RS06255 at 1539.447 kb on + strand at 1539.537 kb on + strand at 1539.702 kb on - strand, within CA265_RS06260 at 1539.786 kb on + strand, within CA265_RS06260 at 1539.847 kb on + strand, within CA265_RS06260 at 1539.848 kb on - strand at 1539.901 kb on - strand, within CA265_RS06260 at 1539.907 kb on + strand, within CA265_RS06260 at 1539.908 kb on - strand, within CA265_RS06260 at 1539.984 kb on + strand, within CA265_RS06260 at 1539.991 kb on + strand, within CA265_RS06260 at 1540.030 kb on - strand, within CA265_RS06260 at 1540.030 kb on - strand, within CA265_RS06260 at 1540.067 kb on + strand, within CA265_RS06260 at 1540.070 kb on - strand, within CA265_RS06260 at 1540.110 kb on + strand, within CA265_RS06260 at 1540.120 kb on - strand, within CA265_RS06260 at 1540.130 kb on - strand, within CA265_RS06260 at 1540.130 kb on - strand, within CA265_RS06260 at 1540.174 kb on - strand at 1540.189 kb on - strand at 1540.215 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.02 mg/ml remove 1,537,725 + +0.1 1,537,741 + -1.0 1,537,808 + CA265_RS06250 0.10 +0.6 1,537,849 + CA265_RS06250 0.17 -0.5 1,538,058 + CA265_RS06250 0.54 -2.6 1,538,241 + CA265_RS06250 0.87 -0.7 1,538,342 + -1.5 1,538,412 - -2.1 1,538,454 + CA265_RS06255 0.12 -2.5 1,538,455 - CA265_RS06255 0.12 -0.5 1,538,520 + CA265_RS06255 0.18 -1.3 1,538,521 - CA265_RS06255 0.18 -1.9 1,538,605 - CA265_RS06255 0.26 -2.0 1,538,664 + CA265_RS06255 0.31 -1.7 1,538,994 - CA265_RS06255 0.61 -1.8 1,539,008 - CA265_RS06255 0.63 -1.7 1,539,036 - CA265_RS06255 0.65 -3.6 1,539,447 + +0.2 1,539,537 + -0.3 1,539,702 - CA265_RS06260 0.19 -1.7 1,539,786 + CA265_RS06260 0.32 -0.7 1,539,847 + CA265_RS06260 0.42 +0.3 1,539,848 - +0.3 1,539,901 - CA265_RS06260 0.50 -0.5 1,539,907 + CA265_RS06260 0.51 -1.0 1,539,908 - CA265_RS06260 0.51 +0.2 1,539,984 + CA265_RS06260 0.63 -1.1 1,539,991 + CA265_RS06260 0.64 +0.1 1,540,030 - CA265_RS06260 0.71 +0.7 1,540,030 - CA265_RS06260 0.71 -0.1 1,540,067 + CA265_RS06260 0.76 -0.6 1,540,070 - CA265_RS06260 0.77 -0.2 1,540,110 + CA265_RS06260 0.83 -0.3 1,540,120 - CA265_RS06260 0.85 -0.3 1,540,130 - CA265_RS06260 0.86 +0.4 1,540,130 - CA265_RS06260 0.86 +0.2 1,540,174 - +0.1 1,540,189 - +0.3 1,540,215 - +0.2
Or see this region's nucleotide sequence