Experiment: R2A with A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS06225 and CA265_RS06230 are separated by 126 nucleotides CA265_RS06230 and CA265_RS06235 are separated by 141 nucleotides CA265_RS06235 and CA265_RS06240 are separated by 130 nucleotides CA265_RS06240 and CA265_RS06245 are separated by 136 nucleotides
CA265_RS06225: CA265_RS06225 - adenylosuccinate lyase, at 1,534,809 to 1,536,155
_RS06225
CA265_RS06230: CA265_RS06230 - hypothetical protein, at 1,536,282 to 1,536,479
_RS06230
CA265_RS06235: CA265_RS06235 - hypothetical protein, at 1,536,621 to 1,536,842
_RS06235
CA265_RS06240: CA265_RS06240 - hypothetical protein, at 1,536,973 to 1,537,191
_RS06240
CA265_RS06245: CA265_RS06245 - hypothetical protein, at 1,537,328 to 1,537,546
_RS06245
Position (kb) 1536
1537 Strain fitness (log2 ratio) -1
0
1 at 1536.203 kb on + strand at 1536.226 kb on - strand at 1536.296 kb on + strand at 1536.348 kb on + strand, within CA265_RS06230 at 1536.372 kb on - strand, within CA265_RS06230 at 1536.372 kb on - strand, within CA265_RS06230 at 1536.662 kb on + strand, within CA265_RS06235 at 1536.722 kb on + strand, within CA265_RS06235 at 1536.858 kb on - strand at 1536.882 kb on + strand at 1536.959 kb on - strand at 1536.964 kb on + strand at 1536.970 kb on - strand at 1536.974 kb on + strand at 1536.975 kb on - strand at 1536.982 kb on - strand at 1537.010 kb on - strand, within CA265_RS06240 at 1537.015 kb on - strand, within CA265_RS06240 at 1537.015 kb on - strand, within CA265_RS06240 at 1537.044 kb on + strand, within CA265_RS06240 at 1537.062 kb on + strand, within CA265_RS06240 at 1537.099 kb on - strand, within CA265_RS06240 at 1537.129 kb on - strand, within CA265_RS06240 at 1537.129 kb on - strand, within CA265_RS06240 at 1537.318 kb on + strand at 1537.318 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.02 mg/ml remove 1,536,203 + -0.5 1,536,226 - +0.9 1,536,296 + -1.2 1,536,348 + CA265_RS06230 0.33 -0.1 1,536,372 - CA265_RS06230 0.45 -1.6 1,536,372 - CA265_RS06230 0.45 -0.9 1,536,662 + CA265_RS06235 0.18 +0.2 1,536,722 + CA265_RS06235 0.45 +1.3 1,536,858 - +0.3 1,536,882 + +0.1 1,536,959 - -0.8 1,536,964 + -0.5 1,536,970 - -1.0 1,536,974 + -0.0 1,536,975 - -0.3 1,536,982 - -0.3 1,537,010 - CA265_RS06240 0.17 +1.0 1,537,015 - CA265_RS06240 0.19 +0.6 1,537,015 - CA265_RS06240 0.19 -1.1 1,537,044 + CA265_RS06240 0.32 -0.4 1,537,062 + CA265_RS06240 0.41 -0.6 1,537,099 - CA265_RS06240 0.58 +0.3 1,537,129 - CA265_RS06240 0.71 -0.4 1,537,129 - CA265_RS06240 0.71 +0.5 1,537,318 + +0.1 1,537,318 + +0.3
Or see this region's nucleotide sequence