Experiment: R2A with A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS05700 and CA265_RS05705 are separated by 14 nucleotides CA265_RS05705 and CA265_RS05710 overlap by 8 nucleotides
CA265_RS05700: CA265_RS05700 - dihydrodipicolinate synthase family protein, at 1,385,832 to 1,386,749
_RS05700
CA265_RS05705: CA265_RS05705 - aldehyde dehydrogenase (NADP(+)), at 1,386,764 to 1,388,350
_RS05705
CA265_RS05710: CA265_RS05710 - hydroxyproline-2-epimerase, at 1,388,343 to 1,389,344
_RS05710
Position (kb)
1386
1387
1388
1389 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1385.918 kb on - strand at 1385.954 kb on + strand, within CA265_RS05700 at 1385.954 kb on + strand, within CA265_RS05700 at 1385.954 kb on + strand, within CA265_RS05700 at 1385.969 kb on + strand, within CA265_RS05700 at 1385.970 kb on - strand, within CA265_RS05700 at 1386.026 kb on + strand, within CA265_RS05700 at 1386.041 kb on - strand, within CA265_RS05700 at 1386.041 kb on - strand at 1386.081 kb on - strand, within CA265_RS05700 at 1386.139 kb on + strand, within CA265_RS05700 at 1386.140 kb on - strand, within CA265_RS05700 at 1386.146 kb on - strand, within CA265_RS05700 at 1386.167 kb on - strand, within CA265_RS05700 at 1386.167 kb on - strand, within CA265_RS05700 at 1386.167 kb on - strand, within CA265_RS05700 at 1386.274 kb on + strand, within CA265_RS05700 at 1386.300 kb on + strand, within CA265_RS05700 at 1386.305 kb on + strand, within CA265_RS05700 at 1386.306 kb on - strand, within CA265_RS05700 at 1386.311 kb on - strand, within CA265_RS05700 at 1386.338 kb on + strand, within CA265_RS05700 at 1386.349 kb on + strand, within CA265_RS05700 at 1386.427 kb on + strand, within CA265_RS05700 at 1386.503 kb on - strand, within CA265_RS05700 at 1386.565 kb on + strand, within CA265_RS05700 at 1386.566 kb on - strand, within CA265_RS05700 at 1386.579 kb on - strand, within CA265_RS05700 at 1386.592 kb on - strand, within CA265_RS05700 at 1386.592 kb on - strand, within CA265_RS05700 at 1386.593 kb on + strand, within CA265_RS05700 at 1386.594 kb on - strand, within CA265_RS05700 at 1386.602 kb on - strand, within CA265_RS05700 at 1386.610 kb on + strand, within CA265_RS05700 at 1386.735 kb on - strand at 1386.745 kb on + strand at 1386.748 kb on - strand at 1386.791 kb on - strand at 1386.795 kb on - strand at 1386.795 kb on - strand at 1386.797 kb on - strand at 1386.799 kb on - strand at 1386.799 kb on - strand at 1386.799 kb on - strand at 1386.799 kb on - strand at 1386.900 kb on + strand at 1386.931 kb on + strand, within CA265_RS05705 at 1387.044 kb on + strand, within CA265_RS05705 at 1387.092 kb on + strand, within CA265_RS05705 at 1387.093 kb on - strand, within CA265_RS05705 at 1387.135 kb on + strand, within CA265_RS05705 at 1387.136 kb on - strand, within CA265_RS05705 at 1387.181 kb on - strand, within CA265_RS05705 at 1387.181 kb on - strand, within CA265_RS05705 at 1387.231 kb on + strand, within CA265_RS05705 at 1387.360 kb on + strand, within CA265_RS05705 at 1387.475 kb on - strand, within CA265_RS05705 at 1387.503 kb on + strand, within CA265_RS05705 at 1387.535 kb on - strand, within CA265_RS05705 at 1387.774 kb on + strand, within CA265_RS05705 at 1387.775 kb on - strand, within CA265_RS05705 at 1387.792 kb on + strand, within CA265_RS05705 at 1387.812 kb on + strand, within CA265_RS05705 at 1388.028 kb on - strand, within CA265_RS05705 at 1388.115 kb on + strand, within CA265_RS05705 at 1388.128 kb on + strand, within CA265_RS05705 at 1388.183 kb on - strand, within CA265_RS05705 at 1388.197 kb on + strand at 1388.320 kb on - strand at 1388.376 kb on - strand at 1388.634 kb on - strand, within CA265_RS05710 at 1388.634 kb on - strand, within CA265_RS05710 at 1388.643 kb on - strand, within CA265_RS05710 at 1388.644 kb on + strand, within CA265_RS05710 at 1388.645 kb on - strand, within CA265_RS05710 at 1388.775 kb on + strand, within CA265_RS05710 at 1388.801 kb on - strand, within CA265_RS05710 at 1388.836 kb on + strand, within CA265_RS05710 at 1388.836 kb on + strand, within CA265_RS05710 at 1388.901 kb on + strand, within CA265_RS05710 at 1388.921 kb on + strand, within CA265_RS05710 at 1388.962 kb on - strand, within CA265_RS05710 at 1388.963 kb on + strand, within CA265_RS05710 at 1388.963 kb on + strand, within CA265_RS05710 at 1388.964 kb on - strand, within CA265_RS05710 at 1388.964 kb on - strand, within CA265_RS05710 at 1388.980 kb on + strand, within CA265_RS05710 at 1389.003 kb on - strand, within CA265_RS05710 at 1389.010 kb on + strand, within CA265_RS05710 at 1389.010 kb on + strand, within CA265_RS05710 at 1389.011 kb on - strand, within CA265_RS05710 at 1389.011 kb on - strand, within CA265_RS05710 at 1389.038 kb on + strand, within CA265_RS05710 at 1389.066 kb on + strand, within CA265_RS05710 at 1389.133 kb on - strand, within CA265_RS05710 at 1389.182 kb on + strand, within CA265_RS05710 at 1389.248 kb on - strand at 1389.257 kb on - strand at 1389.257 kb on - strand at 1389.282 kb on + strand at 1389.299 kb on - strand at 1389.318 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.02 mg/ml remove 1,385,918 - +0.7 1,385,954 + CA265_RS05700 0.13 -0.6 1,385,954 + CA265_RS05700 0.13 -0.9 1,385,954 + CA265_RS05700 0.13 -0.6 1,385,969 + CA265_RS05700 0.15 -1.2 1,385,970 - CA265_RS05700 0.15 +0.1 1,386,026 + CA265_RS05700 0.21 -0.5 1,386,041 - CA265_RS05700 0.23 -0.1 1,386,041 - +0.7 1,386,081 - CA265_RS05700 0.27 +0.5 1,386,139 + CA265_RS05700 0.33 -1.1 1,386,140 - CA265_RS05700 0.34 -0.3 1,386,146 - CA265_RS05700 0.34 +0.7 1,386,167 - CA265_RS05700 0.36 +0.5 1,386,167 - CA265_RS05700 0.36 -0.5 1,386,167 - CA265_RS05700 0.36 +0.7 1,386,274 + CA265_RS05700 0.48 -1.0 1,386,300 + CA265_RS05700 0.51 +0.4 1,386,305 + CA265_RS05700 0.52 -1.0 1,386,306 - CA265_RS05700 0.52 +1.2 1,386,311 - CA265_RS05700 0.52 -0.1 1,386,338 + CA265_RS05700 0.55 -0.2 1,386,349 + CA265_RS05700 0.56 +0.2 1,386,427 + CA265_RS05700 0.65 -1.3 1,386,503 - CA265_RS05700 0.73 -0.2 1,386,565 + CA265_RS05700 0.80 -0.7 1,386,566 - CA265_RS05700 0.80 -0.7 1,386,579 - CA265_RS05700 0.81 -0.1 1,386,592 - CA265_RS05700 0.83 +0.2 1,386,592 - CA265_RS05700 0.83 -0.3 1,386,593 + CA265_RS05700 0.83 +0.6 1,386,594 - CA265_RS05700 0.83 -1.2 1,386,602 - CA265_RS05700 0.84 -0.7 1,386,610 + CA265_RS05700 0.85 -0.1 1,386,735 - -0.2 1,386,745 + +0.2 1,386,748 - +0.4 1,386,791 - -0.0 1,386,795 - -3.3 1,386,795 - -0.1 1,386,797 - +0.2 1,386,799 - +0.1 1,386,799 - -0.6 1,386,799 - -0.1 1,386,799 - +0.4 1,386,900 + -1.7 1,386,931 + CA265_RS05705 0.11 -1.2 1,387,044 + CA265_RS05705 0.18 +1.9 1,387,092 + CA265_RS05705 0.21 -0.3 1,387,093 - CA265_RS05705 0.21 -0.1 1,387,135 + CA265_RS05705 0.23 -0.3 1,387,136 - CA265_RS05705 0.23 -1.1 1,387,181 - CA265_RS05705 0.26 -0.1 1,387,181 - CA265_RS05705 0.26 -0.7 1,387,231 + CA265_RS05705 0.29 -1.9 1,387,360 + CA265_RS05705 0.38 +1.3 1,387,475 - CA265_RS05705 0.45 -1.0 1,387,503 + CA265_RS05705 0.47 -0.6 1,387,535 - CA265_RS05705 0.49 +0.7 1,387,774 + CA265_RS05705 0.64 -1.7 1,387,775 - CA265_RS05705 0.64 -1.1 1,387,792 + CA265_RS05705 0.65 -1.3 1,387,812 + CA265_RS05705 0.66 -1.3 1,388,028 - CA265_RS05705 0.80 -0.5 1,388,115 + CA265_RS05705 0.85 -0.3 1,388,128 + CA265_RS05705 0.86 +1.9 1,388,183 - CA265_RS05705 0.89 +0.6 1,388,197 + -0.4 1,388,320 - +0.3 1,388,376 - +0.3 1,388,634 - CA265_RS05710 0.29 +0.7 1,388,634 - CA265_RS05710 0.29 -0.3 1,388,643 - CA265_RS05710 0.30 +0.2 1,388,644 + CA265_RS05710 0.30 -0.2 1,388,645 - CA265_RS05710 0.30 +0.1 1,388,775 + CA265_RS05710 0.43 -0.9 1,388,801 - CA265_RS05710 0.46 +0.5 1,388,836 + CA265_RS05710 0.49 +0.6 1,388,836 + CA265_RS05710 0.49 +0.1 1,388,901 + CA265_RS05710 0.56 -1.2 1,388,921 + CA265_RS05710 0.58 +0.4 1,388,962 - CA265_RS05710 0.62 +0.2 1,388,963 + CA265_RS05710 0.62 -0.7 1,388,963 + CA265_RS05710 0.62 -0.8 1,388,964 - CA265_RS05710 0.62 -0.0 1,388,964 - CA265_RS05710 0.62 -0.3 1,388,980 + CA265_RS05710 0.64 +1.0 1,389,003 - CA265_RS05710 0.66 +0.1 1,389,010 + CA265_RS05710 0.67 -1.0 1,389,010 + CA265_RS05710 0.67 -0.3 1,389,011 - CA265_RS05710 0.67 +0.4 1,389,011 - CA265_RS05710 0.67 -0.4 1,389,038 + CA265_RS05710 0.69 +1.2 1,389,066 + CA265_RS05710 0.72 +1.7 1,389,133 - CA265_RS05710 0.79 +0.3 1,389,182 + CA265_RS05710 0.84 -0.3 1,389,248 - -0.5 1,389,257 - +0.2 1,389,257 - -0.0 1,389,282 + -0.8 1,389,299 - -0.3 1,389,318 + -0.5
Or see this region's nucleotide sequence